Effects of Egg Shell Membrane Hydrolysates on Skin Whitening, Wound Healing, and UV-Protection

Abstract

This study was conducted to examine the effects of egg shell membrane hydrolysates (ESMH) on skin whitening, wound healing, and UV-protection. ESMH was divided into three groups by molecular weight (Fraction I: above 10 kDa of ESMH, Fraction II: 3 kDa-10 kDa of ESMH, Fraction III: below 3 kDa of ESMH). As a result, all of ESMHs showed over 90% of protein contents. The wound healing experiment using HaCaT cells showed that the fraction I was slightly superior to other fractions depending on the concentration though it was not significantly different. In the experiments of inhibition of tyrosinase and L-3,4-dihydroxyphenylalanine (L-DOPA) oxidation to verify the L-DOPA whitening effect, the whole ESMH (before fractioning) showed a similar amount of inhibition effect with arbutin (control). In the inhibition of melanin formation in B16-F1 melanoma cells, the fraction I showed a high inhibitory effect. In the experiment for protecting the skin from ultraviolet rays using HaCaT cells, all the fractions showed a higher rate of cell viability than the control. In conclusion, this study confirmed that the cosmetic effects of ESMHs such as skin whitening, wound healing, and UV-protection, which were divided depending on the molecule weight. We could confirm that the possibility of ESMHs as a material for functional cosmetics.

Effects of Egg Shell Membrane Hydrolysates on Anti-Inflammatory, Anti-Wrinkle, Anti-Microbial Activity and Moisture-Protection

“…We showed in a previous study that whole eggshell membrane hydrolysates (ESMH) had a skin whitening effect because of their tyrosinase inhibiting and L-DOPA oxidizing activities. Furthermore, we confirmed the wound healing effects of ESMH using HaCaT cells ( Park et al ., 2012 ).…”

“…It was then evaporated and freeze-dried (FD 3, Heto, Gydevang, Denmark). Then, the ESMHs were divided into three fragments: fraction I (>10 kDa), fraction II (3-10 kDa), and fraction III (<3 kDa) as described previously ( Park et al ., 2012 ).…”

“…Keratin is very resistant to physical, chemical, and biological reactions ( Vignardet et al ., 1999 ). ESM has been suggested for use in formulations for moisturizer, wound recovery, skin growth, and anti-wrinkle agents ( Kang, 1997 ; Park et al ., 2012 ). Collagen improved the water content of stratum corneum and normalized transepidermal water loss (TEWL) in UVB-irradiated mice ( Grubauer et al ., 1989 ) and increased the water content of stratum corneum ( Roskos and Guy, 1989 ) and skin viscoelasticity ( Ohara et al ., 2009 ) in humans.…”

Effects of Egg Shell Membrane Hydrolysates on UVB-radiation-induced Wrinkle Formation in SKH-1 Hairless Mice

“…It was then evaporated and freeze-dried (FD 3, Heto, Gydevang, Denmark). Then, the ESMs was divided into three fragments: Fraction I (>10 kDa), Fraction II (3-10 kDa), and Fraction III (<3 kDa) as described previously ( Park et al, 2012 ).…”

Bioactive and Bioadhesive Catechol Conjugated Polymers for Tissue Regeneration

“…Therefore, strict UV protection strategies have been currently advocated during the tissue regeneration process and wound closure [110,111,112]. Different natural compounds such as usnic acid, fern leaves, green tea, retinoids, resveratrol or Cryptomphalus aspersa [113,114,115] have been recently considered as potential UV-blocking sunscreens because of their antioxidant activity or their absorption on the UV region [113]. In this work, our bioinspired catechol-conjugated polymers have been studied as UV skin filters due to their UV absorption with a maximum at 290 nm and their proven antioxidant properties [39,40,41,42].…”