Effects of Egg Shell Membrane Hydrolysates on UVB-radiation-induced Wrinkle Formation in SKH-1 Hairless Mice

Yoo, Jin Hee; Kim, Jong Keun; Yang, Hee-Jong; Park, Ki Moon

doi:10.5851/kosfa.2015.35.1.58

Cited by 16 publications

(21 citation statements)

References 32 publications

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“…Moreover, treatment with eggshells has previously shown a positive effect on skin parameters such as pigmentation and hydration. These effects seem to be mediated by an increase of collagen and HA levels, a reduction of matrix metalloproteinase activity, inhibition of collagenase and elastase, and inhibition of skin inflammation (Yoo et al 2014;Yoo et al, 2015). Although our results showed a tendency toward skin pigmentation reduction, the difference was not statistically significant.…”

Section: Discussioncontrasting

confidence: 74%

“…Eggshell membrane, an organic substance, is known to increase cellular activity, increase collagen production, slow skin aging, and reduce the detrimental effects of damage from UV light and inflammation (Candilish & Scougall,1969;Yoo et al, 2014;Yoo et al, 2015). The objective of this research was to study the effectiveness of Ovoderm, composed of egg membrane, on skin biophysical parameters, such as elasticity, pigmentation, and hydration, related to cutaneous aging.…”

Section: Introductionmentioning

confidence: 99%

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Beneficial Effects of Oral Supplementation With Ovoderm on Human Skin Physiology: Two Pilot Studies

Aguirre¹,

Gil-Quintana²,

Fenaux³

et al. 2017

Journal of Dietary Supplements

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Collagens and hyaluronic acid have long been used in pharmaceuticals and food supplements for the improvement of skin elasticity and hydration. These compounds provide the building blocks of the skin. Ovoderm is an oral supplement obtained from eggshells that contains naturally occurring collagen and glycosaminoglycans, such as hyaluronic acid. We evaluated the efficacy of Ovoderm on skin biophysical parameters related to cutaneous aging such as elasticity, hydration, and pigmentation. Two pilot studies were run to assess the effect of daily oral supplementation with 300 mg Ovoderm on skin parameters. The first consisted of a self-assessment questionnaire intended to perform an assessment on skin, hair, and nail health after 50 days of treatment. The second measured the effect of 5-week treatment on hydration by corneometry, on elasticity with the cutometer, and on pigmentation with the mexameter. In the pilot study 1, participants were predominantly satisfied with the effects obtained on general face (100% volunteers satisfied) and body (94% volunteers satisfied) skin condition and skin properties (100% volunteers satisfied with facial skin softness, 94% with facial skin hydration, and 89% with body skin hydration) and partly with effects on hair (67% volunteers satisfied) and nail (50% volunteers satisfied) condition. The study 2 revealed a statistically significant improvement in skin elasticity (12% increase, p =.0136), a tendency to reduce skin pigmentation (5% decrease), and no significant change in skin hydration. Our study reflects that oral supplementation with Ovoderm is efficacious to reduce the gradual loss of skin elasticity characteristic of aged skin, which helps to improve the appearance of the skin.

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Section: Discussioncontrasting

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Beneficial Effects of Oral Supplementation With Ovoderm on Human Skin Physiology: Two Pilot Studies

Aguirre¹,

Gil-Quintana²,

Fenaux³

et al. 2017

Journal of Dietary Supplements

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“… 16 Of specific interest for the research reported in this article, an earlier study showed that the application of an extract of soluble EM peptides diminished UV-B radiation-induced wrinkle formation in a model of hairless mice, linked to a dose-dependent inhibition of collagenase and also linked to an increase in synthesis of collagen and hyaluronic acid. 17 The aim of this study was to evaluate the effects of soluble EM peptides when applied topically on human skin and to explore specific mechanisms of action pertaining to skin cell matrix deposition and free radical protection at the cellular level.…”

Section: Introductionmentioning

confidence: 99%

Reduction of facial wrinkles by hydrolyzed water-soluble egg membrane associated with reduction of free radical stress and support of matrix production by dermal fibroblasts

Jensen

Shah

Holtz

et al. 2016

CCID

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ObjectiveThe aim of this study was to evaluate the effects of water-soluble egg membrane (WSEM) on wrinkle reduction in a clinical pilot study and to elucidate specific mechanisms of action using primary human immune and dermal cell-based bioassays.MethodsTo evaluate the effects of topical application of WSEM (8%) on human skin, an open-label 8-week study was performed involving 20 healthy females between the age of 45 years and 65 years. High-resolution photography and digital analysis were used to evaluate the wrinkle depth in the facial skin areas beside the eye (crow’s feet). WSEM was tested for total antioxidant capacity and effects on the formation of reactive oxygen species by human polymorphonuclear cells. Human keratinocytes (HaCaT cells) were used for quantitative polymerase chain reaction analysis of the antioxidant response element genes Nqo1, Gclm, Gclc, and Hmox1. Evaluation of effects on human primary dermal fibroblasts in vitro included cellular viability and production of the matrix components collagen and elastin.ResultsTopical use of a WSEM-containing facial cream for 8 weeks resulted in a significant reduction of wrinkle depth (P<0.05). WSEM contained antioxidants and reduced the formation of reactive oxygen species by inflammatory cells in vitro. Despite lack of a quantifiable effect on Nrf2, WSEM induced the gene expression of downstream Nqo1, Gclm, Gclc, and Hmox1 in human keratinocytes. Human dermal fibroblasts treated with WSEM produced more collagen and elastin than untreated cells or cells treated with dbcAMP control. The increase in collagen production was statistically significant (P<0.05).ConclusionThe topical use of WSEM on facial skin significantly reduced the wrinkle depth. The underlying mechanisms of this effect may be related to protection from free radical damage at the cellular level and induction of several antioxidant response elements, combined with stimulation of human dermal fibroblasts to secrete high levels of matrix components.

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“…ESM-P has been reported to have a skin-moisturising effect and to ameliorate knee joint pain [5, 6]. Similar effects have been reported for hyaluronic acid [19, 20].…”

Section: Discussionmentioning

confidence: 80%

“…Eggshell membranes have been used as carbon dioxide and heavy metal absorbers [2, 3], and also in sauces such as soy sauce [4]. They have also been reported to have a skin-moisturising effect and to ameliorate knee joint pain [5, 6]. To clarify these and other potential health functions, it is important to elucidate the action mechanisms of eggshell membrane protein (ESM-P).…”

Section: Introductionmentioning

confidence: 99%

Eggshell membrane protein can be absorbed and utilised in the bodies of rats

Matsuoka¹,

Kurihara²,

Yukawa³

et al. 2019

BMC Res Notes

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Objective Eggshell membranes, the thin film lining the insides of eggshells, are constituted mostly from protein (eggshell membrane protein, ESM-P). The digestibility and dietary net protein utility of ESM-P are not known. ESM-P functions as a barrier to prevent foreign matter from reaching the egg white and yolk, so it would be expected not to decompose easily by digestion when ingested. We therefore prepared a hydrolysate of the membrane (ESM-H). In this study, we assessed the digestibility and net protein utility of ESM-P and ESM-H in rats. Results The digestibility of ESM-P and ESM-H were 87.0% and 94.8%, respectively, significantly lower than that of casein (98.5%). The net protein utility values were 84.7% and 84.6%, respectively, significantly higher than that of casein (75.1%). Digestibility was significantly higher for ESM-H than for ESM-P, but there was no significant difference in net protein utility between ESM-P and ESM-H. These results demonstrated that more than 80% of ESM-P or ESM-H is absorbed and utilised in the bodies of rats.

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Effects of Egg Shell Membrane Hydrolysates on UVB-radiation-induced Wrinkle Formation in SKH-1 Hairless Mice

Cited by 16 publications

References 32 publications

Beneficial Effects of Oral Supplementation With Ovoderm on Human Skin Physiology: Two Pilot Studies

Beneficial Effects of Oral Supplementation With Ovoderm on Human Skin Physiology: Two Pilot Studies

Reduction of facial wrinkles by hydrolyzed water-soluble egg membrane associated with reduction of free radical stress and support of matrix production by dermal fibroblasts

Eggshell membrane protein can be absorbed and utilised in the bodies of rats

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