Effects of electrical stimulation on cytokine‐induced macrophage polarization

Gu, Jiahao; He, Xuzhao; Chen, Xiaoyi; Dong, Lingqing; Weng, Wenjian; Cheng, Kui

doi:10.1002/term.3292

Cited by 21 publications

(17 citation statements)

References 36 publications

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“…45,46 A large number of ion channels, such as the TRPM family and voltage-gated potassium channels, function in the abovementioned signaling cascade and could be upregulated by ES to promote macrophage polarization. 47−49 Combined with previous studies, 22 the potential mechanism by which microstripe morphology and ES synergistically affected macrophages is shown in Figure 6. Only applying ES could upregulate TLR4 and IL-4Rα receptors and activate ion channels (such as Kv1.3 and TRPM7), resulting in promoting both M1 and M2 polarization.…”

Section: Discussionmentioning

confidence: 86%

“…When a sine wave ES was applied, the M2 polarization of macrophages on P(VDF-TrFE)/ITO microelectrodes was promoted (Figures 3 and 4a). This was different from the previous results 22 in which ITO microelectrodes with a sine wave ES promoted both M1 and M2 polarization.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have showed that ES has an effect on the regulation of macrophage functions − (as shown in Table ), including the migration, phagocytosis, and polarization of macrophages. As for the ES modes, dc electric field, piezoelectric surface potential, and ac ES all had some effect on macrophages.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced M2 Polarization of Oriented Macrophages on the P(VDF-TrFE) Film by Coupling with Electrical Stimulation

et al. 2023

ACS Biomater. Sci. Eng.

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Electrical stimulation (ES) has been considered a promising strategy in regulating intracellular communication, membrane depolarization, ion transport, etc. Meanwhile, cell topography, such as the alignment and elongation in anisotropic orientation, also plays a critical role in triggering mechanotransduction as well as the cellular fate. However, coupling of ES and cell orientation to regulate the polarization of macrophages is yet to be explored. In this work, we intended to explore the polarization of macrophages on a poly(vinylidene fluoride-trifluoroethylene [P(VDF-TrFE)] film with intrinsic microstripe roughness, which was covered on indium tin oxide planar microelectrodes. We found that mouse bone marrow-derived macrophages (BMDMs) cultured on a P(VDF-TrFE) film exhibited an elongated morphology aligned with the microstripe crystal whisker, but their polarization behavior was not affected. However, the elongated cells were susceptible to ES and upregulated their M2 polarization, as verified by the related expression of phenotype markers, cytokines, and genes, while not affecting M1 polarization. This is due to the increased expression of the M2 polarization receptor interleukin-4Rα on the surface of elongated BMDMs, while the M1 polarization receptor toll-like receptor 4 was not affected. Thus, M2 polarization was singularly enhanced after activation of polarization by ES. The combination of surface morphology and ES to promote M2 single polarization in this work provides a new perspective for regulating macrophage polarization in the field of immunotherapy.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

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Enhanced M2 Polarization of Oriented Macrophages on the P(VDF-TrFE) Film by Coupling with Electrical Stimulation

et al. 2023

ACS Biomater. Sci. Eng.

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“…The induction medium was changed every two days, and 7 days later, mature BMDMs were seeded on electroactive films. In the previous report, 29 the purity of BMDMs was characterized as 87.9%.…”

Section: Characterizations Of the Chirality-patternedmentioning

confidence: 99%

Regulation of Macrophage Polarization on Chiral Potential Distribution of CFO/P(VDF-TrFE) Films

Zhou

Zhang

et al. 2023

ACS Biomater. Sci. Eng.

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Surface potentials of biomaterials have been shown to regulate cell fate commitment. However, the effects of chirality-patterned potential distribution on macrophage polarization are still only beginning to be explored. In this work, we demonstrated that the chirality-patterned potential distribution of CoFe2O4/poly(vinylidene fluoride-trifluoroethylene) (CFO/P(VDF-TrFE)) films could significantly down-regulate the M1 polarization of bone marrow-derived macrophages (BMDMs). Specifically, the dextral-patterned surface potential distribution simultaneously up-regulated the expression of M2-related markers of BMDMs. The results were attributed to the sensitive difference of integrin subunits (α5β1 and αvβ3) to the dextral- and sinistral-patterned surface potential distribution, respectively. The interaction difference between the integrin subunits and surface potential distribution altered the cell adhesion and cytoskeletal structure and thereby the polarization behavior of BMDMs. This work, therefore, emphasizes the importance of chirality of potential distribution on cell behavior and provides a new strategy to regulate the immune response of biomaterials.

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“…Pacing is a method commonly used with engineered heart tissues derived from primary cardiomyocytes and cardiomyocytes derived from pluripotent stem cell sources 2 , neural applications in the case of neuronal cultures 3 , and skeletal muscle cultures 4 . More recently, the use of electrical pacing has been explored in non-traditional tissues such as skin 5 , bone 6 , and immune cells 7 .…”

Section: Introductionmentioning

confidence: 99%

A guide to the manufacture of sustainable, ready to use in vitro platforms for the electric-field pacing of cellularised 3D porous scaffolds

Monaghan

Solazzo

2022

Preprint

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Electrical activity is a key feature of most native tissues, with the most notable examples being the nervous and the cardiac systems. Modern medicine has moved towards the mimicking and regenerations of such systems both with in vitro models and therapies. Although researchers have now an increased repertoire of cell types and bio-physical cues to generate increasingly complex in vitro models, the inclusion of novel biomaterials in such systems has been negligible, with most approaches relying on scaffold-free self-assembling strategies. However, the rapid development of functional biomaterials and fabrication technologies, such as electroconductive scaffold; warrants consideration and inclusion of materials, with recent evidence supporting the benefit of incorporating electrically active materials and their influence on the maturation of cardiac cells and tissues. In order to be manipulated in bioreactor systems, scaffold-based in vitro models require bespoke rig and bioreactors that vary from those commonly used for scaffold-free systems. In this work, we detail methods to rapid prototype an electrical pacing bioreactor and R3S - a Rig for Stimulation of Sponge-like Scaffolds. As a proof of concept and validation we demonstrate that these systems are compatible with isotropic and anisotropic porous scaffolds composed of collagen or poly(3,4-ethylene dioxythiophene):polystyrene sulfonate (PEDOT:PSS). External pacing of C3H10 cells on anisotropic porous scaffolds led to a metabolic increase and enhanced cell alignment. This setup has been designed for pacing and simultaneously live tracking of in vitro models. This platform has wide suitability for the study of electrical pacing of cellularized scaffolds in 3D in vitro cultures.

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Effects of electrical stimulation on cytokine‐induced macrophage polarization

Cited by 21 publications

References 36 publications

Enhanced M2 Polarization of Oriented Macrophages on the P(VDF-TrFE) Film by Coupling with Electrical Stimulation

Enhanced M2 Polarization of Oriented Macrophages on the P(VDF-TrFE) Film by Coupling with Electrical Stimulation

Regulation of Macrophage Polarization on Chiral Potential Distribution of CFO/P(VDF-TrFE) Films

A guide to the manufacture of sustainable, ready to use in vitro platforms for the electric-field pacing of cellularised 3D porous scaffolds

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