Effects of endocrine disrupting chemicals on gonad development: Mechanistic insights from fish and mammals

Delbès, Géraldine; Blázquez, Mercedes; Fernandino, Juan Ignacio; Grigorova, Petya; Hales, Barbara F.; Metcalfe, Chris D.; Navarro‐Martín, Laia; Parent, Lise; Robaire, Bernard; Rwigemera, Arlette; Kraak, Glen Van Der; Wade, Michael G.; Marlatt, Vicky

doi:10.1016/j.envres.2021.112040

Cited by 80 publications

(36 citation statements)

References 278 publications

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“…For instance, E2 induces sex reversal in P. sinensis and the protandrous barramundi ( Lates calcarifer ) [ 53 , 54 ], and MT controls secondary sexual characteristics in Siniperca chuatsi as well [ 55 ]. The same phenomenon has been confirmed in mammals and fish [ 56 ]. P. sinensis embryo is an excellent model for studying gonadal development and reproduction since development takes place in the egg, and we can intervene directly in vitro .…”

Section: Discussionsupporting

confidence: 70%

Expression and Characterization of the Spats1 Gene and Its Response to E2/MT Treatment in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Luo

Zhu

Chen

et al. 2022

Animals

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Spats1 (spermatogenesis-associated, serinerich 1) has been characterized as a male-biased gene which acts an important role in the germ cell differentiation of mammals. Nevertheless, the function of Spats1 in the Chinese soft-shelled turtle (P. sinensis) has not yet been reported. To initially explore the expression of Spats1 in P. sinensis and its response to sex steroid treatment, we cloned the CDS of Spats1 for the first time and analyzed its expression profile in different tissues, including the testes in different seasons. The Spats1 cDNA fragment is 1201 base pairs (bp) in length and contains an open reading frame (ORF) of 849 bp, which codes for 283 amino acids. Spats1 mRNA was highly expressed in the testes (p < 0.01) and barely detectable in other tissues. In P. sinensis, the relative expression of Spats1 also responsive to seasonal changes in testis development. In summer (July) and autumn (October), Spats1 gene expression was significantly higher in the testes than in other seasons (p < 0.05). Spats1 mRNA was found to be specifically expressed in germ cells by chemical in situ hybridization (CISH), and it was mainly located in primary spermatocytes (Sc1), secondary spermatocytes (Sc2) and spermatozoa (St). Spats1 expression in embryos was not significantly changed after 17α-methyltestosterone (MT)and 17β-estradiol (E2) treatment. In adults, MT significantly induced Spats1 expression in male P. sinensis. However, the expression of Spats1 in testes was not responsive to E2 treatment. In addition, the expression of Spats1 in females was not affected by either MT or E2 treatment. These results imply that Spats1 is a male-specific expressed gene that is mainly regulated by MT and is closely linked to spermatogenesis and release in P. sinensis.

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Section: Discussionsupporting

confidence: 70%

Expression and Characterization of the Spats1 Gene and Its Response to E2/MT Treatment in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Luo

Zhu

Chen

et al. 2022

Animals

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“…Thus, the downregulation of these two transcripts in the testes of SMB during recrudescence could have reproductive implications relating to spermatogenesis in the spring and may play a role in TO development. The molecular mechanisms which regulate testes development are highly conserved among fishes and changes in the regulation of these mechanisms can impact testes development over a lifespan (Delbes et al 2022 ). Changes in TO severity over time may reflect biological responses to contaminant stressors associated with more recent exposures, whereas the prevalence of TO may be more indicative of early life stage effects.…”

Section: Resultsmentioning

confidence: 99%

A case study: temporal trends of environmental stressors and reproductive health of smallmouth bass (Micropterus dolomieu) from a site in the Potomac River Watershed, Maryland, USA

et al. 2022

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Decades of poor reproductive success and young-of-the-year survival, combined with adult mortality events, have led to a decline in the smallmouth bass (SMB; Micropterus dolomieu) population in sections of the Potomac River. Previous studies have identified numerous biologic and environmental stressors associated with negative effects on SMB health. To better understand the impact of these stressors, this study was conducted at the confluence of Antietam Creek and the Potomac River from 2013 to 2019 to identify temporal changes associated with SMB reproductive health. Surface water samples were collected and analyzed for over 300 organic contaminants, including pesticides, phytoestrogens, pharmaceuticals, hormones and total estrogenicity (E2Eq). Adult SMB were collected and sampled for multiple endpoints, including gene transcripts associated with reproduction (molecular), histopathology (cellular), and organosomatic indices (tissue). In males, biomarkers of estrogenic endocrine disruption, including testicular oocytes (TO) and plasma vitellogenin (Vtg) were assessed. Numerous agriculture-related contaminants or land use patterns were associated with gene transcript abundance in both male and female SMB. Positive associations between pesticides in the immediate catchment with TO severity and E2Eq with plasma Vtg in males were identified. In males, the prevalence of TO and detectable levels of plasma Vtg, liver vitellogenin transcripts (vtg) and testis vtg were high throughout the study. Peaks of complex mixtures of numerous contaminants occurred during the spring/early summer when spawning and early development occurs and to a lesser extent in fall/winter during recrudescence. Management practices to reduce exposure during these critical and sensitive periods may enhance reproductive health of these economically important sportfishes.

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“…It is well known that endocrine disruptors have a direct impact on gonads and long-term reproductive health (Delbes et al, 2022). IAA has a negative influence on the female reproductive system, while the effects on the male reproductive system have not been systematically studied.…”

Section: Discussionmentioning

confidence: 99%

Effect of iodoacetic acid on the reproductive system of male mice

Liang

Huang

et al. 2022

Front. Pharmacol.

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Iodoacetic acid (IAA) is one of the most common water disinfection byproducts (DBPs). Humans and animals are widely and continuously exposed to it. Many species of water DBPs are harmful to the reproductive system of organisms. Nevertheless, the potential effects of IAA exposure on testosterone and spermatogenesis in vivo remain ambiguous. Spermatogenous cells are the site of spermatogenesis, Leydig cells are the site of testosterone synthesis, and Sertoli cells build the blood–testis barrier (BTB), providing a stable environment for the aforementioned important physiological functions in testicular tissue. Therefore, we observed the effects of IAA on spermatogenic cells, Leydig cells, and Sertoli cells in the testis. In this study, we found that oral administration of IAA (35 mg/kg body weight per day for 28 days) in male mice increased serum LH levels and reduced sperm motility, affecting average path velocity and straight line velocity of sperm. In addition, IAA promoted the expression of γH2AX, a marker for DNA double-strand breaks. Moreover, IAA downregulated the protein expression of the scavenger receptor class B type 1 (SRB1), and decreased lipid droplet transport into Leydig cells, which reduced the storage of testosterone synthesis raw materials and might cause a drop in testosterone production. Furthermore, IAA did not affect the function of BTB. Thus, our results indicated that IAA exposure affected spermatogenesis and testosterone synthesis by inducing DNA damage and reducing lipid droplet transport.

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Effects of endocrine disrupting chemicals on gonad development: Mechanistic insights from fish and mammals

Cited by 80 publications

References 278 publications

Expression and Characterization of the Spats1 Gene and Its Response to E2/MT Treatment in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Expression and Characterization of the Spats1 Gene and Its Response to E2/MT Treatment in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

A case study: temporal trends of environmental stressors and reproductive health of smallmouth bass (Micropterus dolomieu) from a site in the Potomac River Watershed, Maryland, USA

Effect of iodoacetic acid on the reproductive system of male mice

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