Effects of Ethanol Extracts from Grateloupia elliptica, a Red Seaweed, and Its Chlorophyll Derivative on 3T3-L1 Adipocytes: Suppression of Lipid Accumulation through Downregulation of Adipogenic Protein Expression

Lee, Hyo-Geun; Lu, Yu; Je, Jun-Geon; Jayawardena, Thilina U.; Kang, Min‐Cheol; Lee, Seung‐Hong; Kim, Tae-Hee; Lee, Daesung; Lee, Jeong Min; Yim, Mi‐Jin; Kim, Hyunsoo

doi:10.3390/md19020091

Cited by 19 publications

(9 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, CTEN and GPAR have radical-scavenging activities ( 22 , 23 ). Kim et al ( 24 ) and Lee et al ( 25 ) reported that the bromophenols contained in GELL have antioxidant, anticancer, and anti-inflammatory effects. AANC contains many secondary metabolites, including tannins, which can have antimicrobial and pharmacological activities ( 26 ).…”

Section: Introductionmentioning

confidence: 99%

Red seaweed extracts reduce methane production by altering rumen fermentation and microbial composition in vitro

et al. 2022

View full text Add to dashboard Cite

A series of in vitro batch culture incubations were carried out to investigate changes in rumen fermentation characteristics, methane (CH4) production, and microbial composition in response to supplementation with five different red seaweed species (Amphiroa anceps, AANC; Asparagopsis taxiformis, ATAX; Chondracanthus tenellus, CTEN; Grateloupia elliptica, GELL; and Gracilaria parvispora, GPAR). Prior to the incubations, the total flavonoid and polyphenol content of the red seaweed extracts was quantified. The incubated substrate consisted of timothy hay and corn grain [60:40 dry matter (DM) basis]. Treatments were substrate mixtures without seaweed extract (CON) or substrate mixtures supplemented with 0.25 mg/mL of red seaweed extract. Samples were incubated for 6, 12, 24, 36, and 48 h. Each sample was incubated in triplicates in three separate runs. In vitro DM degradability, fermentation parameters (i.e., pH, volatile fatty acids, and ammonia nitrogen), total gas production, and CH4 production were analyzed for all time points. Microbial composition was analyzed using 16S rRNA amplicon sequencing after 24 h of incubation. The highest CH4 reduction (mL/g DM, mL/g digested DM, and % of total gas production) was observed in ATAX (51.3, 50.1, and 51.5%, respectively, compared to CON; P < 0.001) after 12 h of incubation. The other red seaweed extracts reduced the CH4 production (mL/g DM; P < 0.001) in the range of 4.6–35.0% compared to CON after 24 h of incubation. After 24 h of incubation, supplementation with red seaweed extracts tended to increase the molar proportion of propionate (P = 0.057) and decreased the acetate to propionate ratio (P = 0.033) compared to the CON. Abundances of the genus Methanobrevibacter and total methanogens were reduced (P = 0.050 and P = 0.016) by red seaweed extract supplementation. The linear discriminant analysis effect size (P < 0.05, LDA ≥ 2.0) showed that UG Succinivibrionaceae, Anaeroplasma, and UG Ruminococcaceae, which are associated with higher propionate production, starch degradation, and amylase activity were relatively more abundant in red seaweed extracts than in the CON. Our results suggest that supplementation with red seaweed extracts altered the microbiota, leading to the acceleration of propionate production and reduction in CH4 production.

show abstract

Section: Introductionmentioning

confidence: 99%

Red seaweed extracts reduce methane production by altering rumen fermentation and microbial composition in vitro

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In the search for bioactive compounds for sustainable use of Grateloupia spp., some studies have reported the evaluation of bioactivities of organic solvent extracts of G. turuturu (also reported in the literature as G. doryphora ) and species in the same genus, such as G. filicina , G. elliptica , G. imbricata and G. lanceola [ 7 , 31 , 32 , 33 , 34 ]. Methanol, acetone, ethyl acetate and chloroform extracts from G. filicina have shown good antioxidants properties as scavengers of reactive oxygen species [ 35 ] and capacities of reducing lipid peroxidation in oils and DNA damage in rat lymphocytes [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

Polar Lipids Composition, Antioxidant and Anti-Inflammatory Activities of the Atlantic Red Seaweed Grateloupia turuturu

et al. 2021

View full text Add to dashboard Cite

Grateloupia turuturu Yamada, 1941, is a red seaweed widely used for food in Japan and Korea which was recorded on the Atlantic Coast of Europe about twenty years ago. This seaweed presents eicosapentaenoic acid (EPA) and other polyunsaturated fatty acids (PUFAs) in its lipid fraction, a feature that sparked the interest on its potential applications. In seaweeds, PUFAs are mostly esterified to polar lipids, emerging as healthy phytochemicals. However, to date, these biomolecules are still unknown for G. turuturu. The present work aimed to identify the polar lipid profile of G. turuturu, using modern lipidomics approaches based on high performance liquid chromatography coupled to high resolution mass spectrometry (LC–MS) and gas chromatography coupled to mass spectrometry (GC–MS). The health benefits of polar lipids were identified by health lipid indices and the assessment of antioxidant and anti-inflammatory activities. The polar lipids profile identified from G. turuturu included 205 lipid species distributed over glycolipids, phospholipids, betaine lipids and phosphosphingolipids, which featured a high number of lipid species with EPA and PUFAs. The nutritional value of G. turuturu has been shown by its protein content, fatty acyl composition and health lipid indices, thus confirming G. turuturu as an alternative source of protein and lipids. Some of the lipid species assigned were associated to biological activity, as polar lipid extracts showed antioxidant activity evidenced by free radical scavenging potential for the 2,2′-azino-bis-3-ethyl benzothiazoline-6-sulfonic acid (ABTS●+) radical (IC50 ca. 130.4 μg mL−1) and for the 2,2-diphenyl-1-picrylhydrazyl (DPPH●) radical (IC25 ca. 129.1 μg mL−1) and anti-inflammatory activity by inhibition of the COX-2 enzyme (IC50 ca. 33 µg mL−1). Both antioxidant and anti-inflammatory activities were detected using a low concentration of extracts. This integrative approach contributes to increase the knowledge of G. turuturu as a species capable of providing nutrients and bioactive molecules with potential applications in the nutraceutical, pharmaceutical and cosmeceutical industries.

show abstract

“…Chlorophyll is the pigment present in plant parts that has been proved to have medicinal properties. The chlorophyll derivatives influence the metabolism of lipids in a positive manner, which can be further used in the management of obesity [ 13 ]. C. sativum leaves have been shown to have the highest concentrations of about 14 µg/mL and the lowest of about 9.5 µg/mL of chlorophyll [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

Efficiency of Coriandrum sativum (Linn.) and Petroselinum crispum (Mill.) in Enhancing Iron Absorption: An In Silico and In Vitro Approach

Sangeetha

Ibrahim²,

Deepa³

et al. 2022

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Coriandrum sativum (Linn.) and Petroselinum crispum (Mill.) are the common herbs used for culinary purposes in daily life. The chlorophyll pigment in plants is being identified with various medicinal values, whereas iron is an essential micronutrient for the proper metabolism of the human body. The current research has been aimed at predicting the role of C. sativum and P. crispum in enhancing iron absorption via an in vitro approach. C. sativum and P. crispum have been analyzed for their capability of being a source of chlorophyll and iron concentration. The extracts prepared from solvents like carbinol, petroleum ether, and water were subjected to the identification of phytoconstituents through gas chromatography-mass spectrometry analysis, and the identified compounds were subjected to in silico studies against the iron-binding receptor, transferrin, to depict the binding affinity of the identified compounds. The carbinol extract was then put through in vitro analytical studies in Caco2 cell lines with a concentration of 500 µg/ml. Current research has shown that the leaves of C. sativum and P. crispum are an excellent source of chlorophyll and iron and has also suggested that these herbs efficiently enhance the absorption of iron in human intestinal cells.

show abstract

Effects of Ethanol Extracts from Grateloupia elliptica, a Red Seaweed, and Its Chlorophyll Derivative on 3T3-L1 Adipocytes: Suppression of Lipid Accumulation through Downregulation of Adipogenic Protein Expression

Cited by 19 publications

References 74 publications

Red seaweed extracts reduce methane production by altering rumen fermentation and microbial composition in vitro

Red seaweed extracts reduce methane production by altering rumen fermentation and microbial composition in vitro

Polar Lipids Composition, Antioxidant and Anti-Inflammatory Activities of the Atlantic Red Seaweed Grateloupia turuturu

Efficiency of Coriandrum sativum (Linn.) and Petroselinum crispum (Mill.) in Enhancing Iron Absorption: An In Silico and In Vitro Approach

Contact Info

Product

Resources

About