Effects of euthanasia methods on stable carbon (δ13C value) and nitrogen (δ15N value) isotopic compositions of fry and juvenile rainbow trout <scp>Oncorhynchus mykiss</scp>

Nahon, Sarah; Séité, Sarah; Kolasinski, Joanna; Aguirre, Pierre; Geurden, Inge

doi:10.1002/rcm.7958

Cited by 10 publications

(3 citation statements)

References 29 publications

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“…Uneaten feed and faeces were removed from the tanks at the end of each feeding period. After 16 hr of fasting (the time necessary for fry guts to empty), six fry were randomly sampled from each tank, carefully rinsed in ultra‐pure water (milli‐Q ® , Merck‐Millipore, Molsheim, France), killed by immersion in an ice slurry, freeze dried, and then stored at −80°C until analysis (Nahon, Séité, Kolasinski, Aguirre, & Geurden, 2017). Fry were sampled daily from day 0–15, every second day from day 16–31, and every week from day 32–36.…”

Section: Methodsmentioning

confidence: 99%

How protein quality drives incorporation rates and trophic discrimination of carbon and nitrogen stable isotope ratios in a freshwater first‐feeding fish

et al. 2020

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1. Using stable isotope ratios to explore the trophic ecology of freshwater animals requires knowledge about effects of food quality on isotopic incorporation dynamics. The aim of this experimental study was to: (1) estimate carbon and nitrogen isotopic incorporation rates and trophic discrimination factors (TDFs) of a freshwater first-feeding fish (i.e. salmonid fry) fed three diets that differed only in protein quality (animal or plant or a blend of both); (2) investigate effects of fasting and; (3) evaluate the proportion of each source assimilated when fry were fed a 50:50 animal:plant-based diet. 2. For each diet, incorporation rates of δ 13 C and δ 15 N values were estimated using a time or growth-dependent isotopic incorporation model. Effects of fasting on isotope ratio values were measured regularly until the death of fry. Bayesian stableisotope mixing models were used to estimate the contribution of animal and plant material to fish fed a blend of both food types. 3. Our results show that incorporation rates were lower for fry fed a plant-based diet than for those fed an animal-based diet as growth rate decreased. Timeand growth-dependent models indicated that growth was solely responsible for isotopic incorporation in fry fed an animal-based diet, whereas catabolism increased in fry fed a plant-based diet. After lipid extraction, carbon TDFs were similar regardless of the diet, whereas nitrogen TDFs increased for fry fed a plantbased diet. Long-term fasting induced an increase of 0.63‰ in δ 13 C values of fry in 23 days, whereas δ 15 N values did not vary significantly. Proportions of food sources assimilated by fry fed an animal:plant-based diet were similar to those consumed when using a mixing model with the estimated TDFs, while proportions were unrealistic when using mean TDFs extrapolated from the literature. 4. The results of our study indicate that the quality of food must be considered to use an appropriate timescale to detect changes in fry diets in the field. Moreover, we recommend using different carbon and nitrogen TDFs, one for animal-derived | 1871 NAHON et Al.

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Section: Methodsmentioning

confidence: 99%

How protein quality drives incorporation rates and trophic discrimination of carbon and nitrogen stable isotope ratios in a freshwater first‐feeding fish

et al. 2020

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“…The exemplars were euthanized with eugenol (0.4 ml/l of eugenol solution 1:10 in ethanol 70%) for subsequent collection of muscle tissue for analysis of carbon and nitrogen stable isotope ratios (δ 13 C and δ 15 N, respectively). This anaesthetic was used because it does not significantly alter stable isotopic composition (Nahon et al, 2017). Only the individuals collected in March were used in the SIA because their isotopic turnover reflects food assimilation in the prior two months of sampling (Oliveira et al, 2017) and thus the time that both species co-occur in the study system.…”

Section: Stable Isotopic Analysismentioning

confidence: 99%

Trophic niches and diet shifts of juvenile mullet species coexisting in marine and estuarine habitats

et al. 2021

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Food partitioning among coexisting species is often considered advantageous to minimize niche overlap and avoid inter-specific competition. Congeneric fish species such as the mullets Mugil curema and Mugil liza, which co-occur across marine and estuarine habitats, are good models to evaluate resource use and niche overlap or partitioning. We used stomach contents (SCA) and stable isotope analysis (SIA) to assess potential trophic shifts and changes in niche overlap associated with the mullets transitioning from marine to estuarine habitats. SIA included different fractions of organic matter in suspension and in the sediment to estimate the contribution of micro, nano and pico-organisms to the mullets’ diets. We hypothesized higher resource partitioning in the less resource-diverse system (marine surf-zone) than in the more diverse one (estuary). SCA showed diet differences between M. curema and M. liza according to the habitat. They showed distinct diets in the marine area (P < 0.001), but similar diets in the estuary (P = 0.226). A lower niche breadth was observed for both species in the marine area (M. curema = 0.03, M. liza = 0.06) compared with the estuary (M. curema = 0.14, M. liza = 0.16). Isotopic niches of both species were higher in the estuary (64.7%) compared with the marine area (0.7%). These findings corroborated our hypothesis of higher food partitioning in the marine surf-zone. We also demonstrated using SIA the shift from planktonic to benthic feeding following the recruitment of the mullets from the surf-zone into the estuary.

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“…Specifically, we collected a total of n = 85 specimens for Pyrenean minnow (30-68 mm TL), n = 60 for brown trout (163-199 mm TL), n = 25 for Pyrenean gudgeon (30-110 mm TL), n = 20 for Pyrenean stone loach (50-100 mm TL), and n = 10 for Ebro nase (84-119 mm TL). All of the collected fish and crayfish were properly euthanized with low temperatures (cooler), as the use of anesthetic was not recommended for the subsequent stable isotope analysis (SIA) [35]. Crayfish and fish individuals were stored in ice during transport to the laboratory on the same date of collection (<2 h since capture).…”

Section: Trophic Overlapmentioning

confidence: 99%

Behavioral Interactions and Trophic Overlap between Invasive Signal Crayfish Pacifastacus leniusculus (Decapoda, Astacidae) and Native Fishes in Iberian Rivers

Vedia

Almeida

Rodeles

et al. 2019

Water

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The signal crayfish Pacifastacus leniusculus Dana, 1852 is a successful invasive species in the Iberian Peninsula. This is of particular conservation concern, as fish fauna is highly endemic and also threatened within this ecoregion. The aim of this study was to analyze behavioral interactions and trophic overlap between signal crayfish and native fishes in Iberian rivers (northern Spain). Video cameras were used to record fish “dominance/evasion” after spatial encounters with crayfish. Diet composition and isotopic signatures (δ13C and δ15N) were compared to evaluate the niche overlap. Fish were dominant in 25% of the encounters with juvenile crayfish, whereas this percentage was only 4% with adult crayfish. Observations showed a high fish “evasion” response for Pyrenean stone loach Barbatula quignardi (Bǎcescu-Meşter, 1967) (>30%). Dietary results showed a high trophic overlap between signal crayfish with the pelagic Pyrenean minnow Phoxinus bigerri Kottelat, 2007 and the benthic loach. However, the isotopic niche overlap was low, with brown trout Salmo trutta L., 1758 showing the highest area (only 0.1 ‰2). Overall, our findings suggest that interferences may occur with native species for food (i.e., benthic invertebrates). Consequently, measures should be applied to control invasive crayfish in Iberian rivers.

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Effects of euthanasia methods on stable carbon (δ¹³C value) and nitrogen (δ¹⁵N value) isotopic compositions of fry and juvenile rainbow trout Oncorhynchus mykiss

Cited by 10 publications

References 29 publications

How protein quality drives incorporation rates and trophic discrimination of carbon and nitrogen stable isotope ratios in a freshwater first‐feeding fish

How protein quality drives incorporation rates and trophic discrimination of carbon and nitrogen stable isotope ratios in a freshwater first‐feeding fish

Trophic niches and diet shifts of juvenile mullet species coexisting in marine and estuarine habitats

Behavioral Interactions and Trophic Overlap between Invasive Signal Crayfish Pacifastacus leniusculus (Decapoda, Astacidae) and Native Fishes in Iberian Rivers

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Effects of euthanasia methods on stable carbon (δ13C value) and nitrogen (δ15N value) isotopic compositions of fry and juvenile rainbow trout Oncorhynchus mykiss

Cited by 10 publications

References 29 publications

How protein quality drives incorporation rates and trophic discrimination of carbon and nitrogen stable isotope ratios in a freshwater first‐feeding fish

How protein quality drives incorporation rates and trophic discrimination of carbon and nitrogen stable isotope ratios in a freshwater first‐feeding fish

Trophic niches and diet shifts of juvenile mullet species coexisting in marine and estuarine habitats

Behavioral Interactions and Trophic Overlap between Invasive Signal Crayfish Pacifastacus leniusculus (Decapoda, Astacidae) and Native Fishes in Iberian Rivers

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Effects of euthanasia methods on stable carbon (δ¹³C value) and nitrogen (δ¹⁵N value) isotopic compositions of fry and juvenile rainbow trout Oncorhynchus mykiss