Effects of exercise training together with tamoxifen in reducing mammary tumor burden in mice: Possible underlying pathway of miR-21

Khori, Vahid; Amani-Shalamzari, Sadegh; Isanejad, Amin; Alizadeh, Ali Mohammad; Alizadeh, Shaban; Khodayari, Saeed; Khodayari, Hamid; Shahbazi, Shirin; Zahedi, Ali; Sohanaki, Hamid; Khaniki, Mahmood; Mahdian, Reza; Saffari, Mojtaba; Fayad, Raja

doi:10.1016/j.ejphar.2015.08.031

Cited by 65 publications

(55 citation statements)

References 48 publications

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“…Relative pixel density was calculated for each band following background subtraction (Khori et al . ).…”

Section: Methodsmentioning

confidence: 97%

“…Mouse mammary adenocarcinoma tumours (derived from MC4-L2 as a stock model; Khori et al 2015) were aseptically separated from the mammary-cancer-bearing BALB/c mice, cut into pieces of less than 0.2 ̶ 0.3 cm 3 , and subcutaneously transplanted into the animals right flank under ketamine (100 mg kg −1 , I.P) and xylazine (10 mg kg −1 , I.P) anaesthesia (Farhangi et al 2015).…”

Section: Tumour Transplantationmentioning

confidence: 99%

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Oxytocin mediates the beneficial effects of the exercise training on breast cancer

Alizadeh

Heydari

Rahimi

et al. 2017

Experimental Physiology

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What is the central question of this study? We hypothesized that potential anti-tumour effects of exercise training might be mediated by oxytocin and explored the underlying mechanisms in a mouse model of breast cancer. What is the main finding and its importance? Interval exercise training, by inducing oxytocin secretion, may reduce the activity of the PI3K/Akt and ERK pathways, and consequently, results in a smaller tumour volume in a mouse model of breast cancer. Exercise training can affect the growth of breast tumours. We hypothesized that exercise training might reduce breast tumour growth by inducing oxytocin (OT) secretion and its related signalling pathways, such as PI3K/Akt and ERK. Therefore, 56 BALB/c mice were equally divided into seven groups to study the effects of OT and atosiban (an oxytocin receptor antagonist) together with interval exercise training on mammary tumour growth, as well as tumour-related signalling pathways, including PI3K/Akt and ERK. Animal weight, OT plasma concentration, tumour weight and volume were measured at the end of the study. PI3K/Akt and ERK were evaluated by Western blot and qPCR assays. The results showed that OT plasma concentration was significantly increased in trained animals. The volume and weight of tumours were decreased significantly after both exercise training and OT administration. The expression of genes involved in tumour cell proliferation, such as PI3KR2, Akt and mTOR, was notably lower in the exercise-trained and OT-treated groups. Furthermore, the expression of genes involved in cell apoptosis, such as caspase-3 and Bax, was significantly increased in the tumour tissues. In addition, Western blot results showed that phosphorylated Akt and ERK were significantly decreased in the exercise training and OT groups compared with the tumour group. Interestingly, atosiban reversed these effects. These results indicated that interval exercise training, acting via OT secretion, may reduce PI3K/Akt and ERK axis activities, and consequently, decrease tumour volume and weight in a mouse model of breast cancer.

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“…Relative pixel density was calculated for each band following background subtraction (Khori et al . ).…”

Section: Methodsmentioning

confidence: 97%

Section: Tumour Transplantationmentioning

confidence: 99%

Oxytocin mediates the beneficial effects of the exercise training on breast cancer

Alizadeh

Heydari

Rahimi

et al. 2017

Experimental Physiology

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“…23 It was also proposed to be a crucial tumor suppressor, which exhibited a low expression level in many solid tumors. 24,25 However, TPM1 exon 6a was found exclusively included in the prostate cancer samples but preferentially included in bladder cancer samples, which enlightened us a possible controversy of its role. 26 Therefore, this study investigated a TPM1 network to explore its specific function in BC.…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA MEG3 suppresses the development of bladder urothelial carcinoma by regulating miR-96 and TPM1

Liu

Zhao

Zhou

et al. 2018

Cancer Biology & Therapy

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We aimed at investigating effects of long non-coding RNA maternally expressed 3 (MEG3) on the proliferation, cell cycle and apoptosis of bladder urothelial carcinoma cells and regulatory relationships among lncRNA MEG3, miR-96 and α-tropomyosin 1 (TPM1). Human clinical data from The Cancer Genome Atlas (TCGA) which contains bladder urothelial carcinoma tissues and adjacent tissues were used for analysis. The expression profiles of MEG3, miR-96, TPM1, cell cycle-related genes and apoptosis-related genes were examined by real-time quantitative polymerase chain reaction (RT-qPCR) and western blot. Regulating relationship among MEG3, miR-96 and TPM1 was confirmed by dual luciferase reporter assay. MTT assay and flow cytometry were performed to observe cell proliferation, cell cycle and apoptosis. The effects of lncRNA MEG3 on bladder urothelial carcinoma were confirmed both in vivo and in vitro. The mRNA expression and protein expression of MEG3, TPM1 were down-regulated in carcinoma tissues, whereas miR-96 expression was up-regulated. MEG3 overexpression resulted in miR-96 downregulation along with TPM1 upregulation, which inhibited cell proliferation and cell cycle but promoted cell apoptosis of bladder urothelial carcinoma cells in vitro, and at the same time inhibited tumor growth in vivo. In this process, expressions of apoptosis-related protein BCL2 associated X (Bax), cleaved-caspase 3 was up-regulated, whereas apoptosis regulator protein (Bcl-2) expression was suppressed when MEG3 was overexpressed, and cell cycle-related protein Cyclin D1 was down-regulated. LncRNA MEG3 low-expression promotes the proliferation and inhibits apoptosis of bladder urothelial carcinoma cells by regulating miR-96 along with TPM1. ARTICLE HISTORY

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“…A previous study showed that voluntary exercise suppresses miR-132 expression in the hippocampus of SAMP8 mice, which is associated with cognitive improvement (Dong et al, 2018). In addition, both exercise and tamoxifen have synergistic effects in reducing the expression of miR-21 in an estrogen receptor-positive breast cancer model to reduce mammary tumor burden in mice (Khori et al, 2015). These studies suggest that exercise may have positive effects on pathological conditions through the miR-132/miR-21 pathway.…”

Section: Figure 7 | Correlation Between Mir-34a Expression and Bdnf Ementioning

confidence: 80%

Excessive Treadmill Training Enhances Brain-Specific MicroRNA-34a in the Mouse Hippocampus

Zheng

Yin

et al. 2020

Front. Mol. Neurosci.

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Background: An imbalance between total training load and total recovery may cause overtraining (OT). The purpose of the present study was to verify the effects of OT on the expression of brain-derived neurotrophic factor (BDNF), its receptor tropomyosin receptor kinase B (TrkB) and p75 and the dynamic expression patterns of brainspecific miR-34a and miR-124 or inflammation-related miR-21 and miR-132 in the mouse hippocampus.Method: Eight weeks old C57BL/6J mice were randomly assigned to the control (CON), normal training (NT) and OT groups. An 8-week OT training protocol was applied to evaluate the phenotype of mice endurance (incremental load test, ILT) and cognitive capacity (Morris water maze test). We used qRT-PCR and immunoblotting to detect changes in the molecular level of hippocampal samples.Result: Compared with the CON, both NT and OT decreased bodyweight after 8-week training. After 8-week of training, NT increased the exhaustion velocity (EV) while the EV of OT was lower than NT. Mice in NT decreased the escape latency than CON. The percentage of time spent in the probe quadrant and the number of crossing platform times in NT were higher than CON and OT. The BDNF, p75 and TrkB mRNA levels were increased in NT than CON, only the p75 mRNA was increased in OT. The NT exhibited increased protein levels of BDNF and TrkB compared to CON. The protein expression of BDNF was decreased in OT than NT and CON. The protein level of p75 in the OT was higher than in NT and CON. In addition, the phosphorylation level of TrkB in OT was higher than CON and NT. Only the miR-34a level was increased in the OT. Moreover, the expression of miR-34a was found to be negatively correlated with the expression of BDNF, and the increase in miR-34a level was accompanied by a decrease in performance.Frontiers in Molecular Neuroscience | www.frontiersin.org January 2020 | Volume 13 | Article 7 Xu et al. Brain-Specific MicroRNA-34a and Excessive TrainingConclusion: In summary, the training-evoked increase in the BDNF level may help to improve performance, whereas this conditioning is lost after OT. Moreover, miR-34a potentially mediated changes in the expression of BDNF and may reflect the decrease in performance after OT.

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Effects of exercise training together with tamoxifen in reducing mammary tumor burden in mice: Possible underlying pathway of miR-21

Cited by 65 publications

References 48 publications

Oxytocin mediates the beneficial effects of the exercise training on breast cancer

Oxytocin mediates the beneficial effects of the exercise training on breast cancer

Long non-coding RNA MEG3 suppresses the development of bladder urothelial carcinoma by regulating miR-96 and TPM1

Excessive Treadmill Training Enhances Brain-Specific MicroRNA-34a in the Mouse Hippocampus

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