Effects of Exogenous Glutathione on the Quality of Chilled Bull Semen

Abstract

Glutathione (GSH) at concentrations of 0.0 (control), 0.5, 1.0, 2.0 and 3.0 mM was added to chilled bull semen to determine its effects on the keeping quality of semen used for artificial insemination (AI). The semen was preserved with egg yolk citrate extender. All samples were stored at 4-8 degrees C for 5 days. Sperm motility and proportion of abnormal acrosome were assessed daily. Sperm motility was significantly (p < 0.01) higher in the semen treated with 0.5 mM glutathione than in untreated semen on each day. The optimum sperm motility (>or=50%) for AI was retained significantly (p < 0.01) for 3 days in 0.0, 0.5, 1.0 and 2.0 mM glutathione treated semen, whereas in 0.3 mM glutathione-treated semen, sperm motility was 46.8% for 3 days. Acrosomal damage was significantly (p < 0.01) reduced after addition of 0.5 mM GSH in the preserved semen. Bull semen can be preserved in chilled condition for 5 days with 0.5 mM GSH with sperm motility above 40% and 12% acrosome abnormality.

Reduced glutathione and cysteine hydrochloride on crossbred bull semen

“…The present finding has correlated with the findings of Sagdeo et al (1990) in 75 percent JY, 62.5 percent JY bulls. Higher rating was recorded by Sagdeo et al (1990) in Pure JY bulls, Raina and Dhami, (2004) in Gir bulls and Munsi et al(2007) in HF crossbred bull, where as, lower values than the present investigation were also reported by Sagdeo et al (1990) in 50 percent JY, 75 percent HF and 62.50 percent HF crossbred bulls. In the present investigation, the average rating of mass motility was more than two; hence the extension of semen was tenable.…”

“…The present observation of higher motility in glutathione added semen is comparable to the findings of Jain and Arora (1988) and . However slightly lower value has also been reported by Munsi et al (2007) and Zekariya Nur et al (2005), who reported significantly higher motility percent in glutathione added semen in crossbred bulls. It has been observed that a spermatozoon in liquid medium is propelled forward by flagellar beating initiated from distal centriole through electron transport.…”

“…8 ± 0.3, 8.3 ± 0.6, 11.7 ± 0.7, 15.4 ± 0.9 and 20.5 ± 1.0 respectively. The proportion of acrosomal abnormality was significantly lower (12%) in 0.5 mM GSH treated semen on Day 5 than in other groups (Munsi et al,2007). Drevius and Eriksson (1966) demonstrated the swelling phenomena of sperm tail of bovine spermatozoa when exposed to a hypo osmotic solution (0.024M to 0.043M sodium chloride solution).…”

“…On Day 3 (54.0%), 4 (46.4%) and 5 (40.3%), 0.5 mM GSH treatment showed significantly better sperm motility, but the concentrations of 0.0, 2.0 and 3.0 mM 15 GSH had 51.5%, 49.6% and 46.8% sperm motility, respectively. Sperm motility in semen treated with 0.5, 1.0, 2.0 and 3.0 mM GSH, decreased by about 4%, 6%, 8% and 9%, respectively, each day, but by about 6% each day in the control group (Munsi et al, 2007).…”

Selênio para caprinos e ovinos:Revisão

“…Portanto, animais com deficiência de selênio podem ter suprimida a capacidade de defesa contra doenças infecciosas, que poderiam prejudicar a sobrevivência do embrião (Ashworth 1995). Munsi et al (2007) verificaram melhora na mortalidade espermática progressiva e redução das anormalidades espermáticas de animais tratados com glutationa peroxidase, comparados aos não tratados. O selênio é considerado um antioxidante, tendo como função o co-fator da glutationa peroxidase, enzima responsável pelo sistema de regulação extra e intracelular da hidroperoxidase (Luberda 2005).…”