Effects of fatty acid supplementation during vitrification and warming on the developmental competence of mouse, bovine and human oocytes and embryos

Ohata, Kazuki; Ezoe, Kenji; Miki, Tetsuya; Kouraba, Shizu; Fujiwara, Nanoha; Yabuuchi, Akiko; Kobayashi, Tamotsu; Kato, Keiichi

doi:10.1016/j.rbmo.2021.03.022

Cited by 16 publications

(20 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The blastocysts in the control and biopsy groups at 0, 1, and 4 h after TE biopsy were transferred to the uteri of pseudo‐pregnant female Institute of Cancer Research (ICR) mice. 15 The pseudo‐pregnancy mice were mated vasectomized ICR male mice to induce pseudo‐pregnancy (Day1 = vaginal plug). Nine to twelve blastocysts were transferred into the uteri at 3 days post‐coitum, and the pregnant females were sacrificed at 19 days post‐coitum.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Time from trophectoderm biopsy to vitrification affects the developmental competence of biopsied blastocysts

Miki¹,

Ezoe²,

Kouraba³

et al. 2022

Reprod Medicine & Biology

Self Cite

View full text Add to dashboard Cite

Purpose The present study aimed to examine the correlations of the time interval from trophectoderm (TE) biopsy to vitrification with the blastocyst survival rate and blastocyst outgrowth ability. Methods A total of 1,202 mouse blastocysts were randomly divided into control (non‐biopsy) and TE biopsy groups. The biopsied blastocysts were vitrified at various time points. The survival rate after warming, blastocyst adhesion rate, and outgrowth area was investigated. Several biopsied blastocysts were cultured in a time‐lapse incubator, and the time required for re‐expansion was measured. Results Blastocyst survival rates after warming and blastocyst adhesion rates were comparable between the control and biopsy groups. The area of trophoblast outgrowth in the 1‐h biopsy group was significantly smaller than that in the control, 0‐h biopsy, and 4‐h biopsy groups ( p = 0.0304, p = 0.0058, and p = 0.0029, respectively). Re‐expansion of blastocysts was observed at a high incidence 1–2 h after TE biopsy. Conclusions The vitrification of biopsied blastocysts in the process of re‐expansion impairs outgrowth competence; therefore, blastocyst vitrification should be performed immediately after TE biopsy and before initiation of re‐expansion.

show abstract

Section: Methodsmentioning

confidence: 99%

“…The blastocysts in the control and biopsy groups at 0, 1, and 4 h after TE biopsy were transferred to the uteri of pseudo‐pregnant female Institute of Cancer Research (ICR) mice 15 …”

Section: Methodsmentioning

confidence: 99%

Time from trophectoderm biopsy to vitrification affects the developmental competence of biopsied blastocysts

Miki¹,

Ezoe²,

Kouraba³

et al. 2022

Reprod Medicine & Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“… 18 , 19 , 20 , 21 Our recent study reported that the vitrification procedure decreased the intracellular lipid content and subsequent developmental competence. 22 In addition, the supplementation of fatty acid (FA) into the warming solution increased the intracellular lipid content and improved the developmental competence by stimulating the β‐oxidation pathway in mice and bovine. We also demonstrated that FA addition during warming improved the developmental competence of vitrified‐warmed 4‐cell stage embryos, leading to the increase of outgrowth competence in humans.…”

Section: Introductionmentioning

confidence: 99%

Fatty acid supplementation into warming solutions improves pregnancy outcomes after single vitrified‐warmed cleavage stage embryo transfers

Amagai¹,

Ezoe²,

Miki³

et al. 2023

Reprod Medicine & Biology

Self Cite

View full text Add to dashboard Cite

Purpose This study aimed to examine the embryonic development of human 4‐cell stage embryos after warming with fatty acids (FAs) and to assess the pregnancy outcomes after single vitrified‐warmed cleavage stage embryo transfers (SVCTs). Methods Experimental study: A total of 217 discarded, vitrified human 4‐cell stage embryos donated for research by consenting couples were used. The embryos were warmed using the fatty acid (FA)‐supplemented solutions (FA group) or nonsupplemented solutions (control group). The developmental rate, morphokinetics, and outgrowth competence were analyzed. Clinical study: The treatment records of women undergoing SVCT in natural cycles between April and September 2022 were retrospectively analyzed (April–June 2022, control group; July–September 2022, FA group). Results Experimental study: The rate of morphologically good blastocysts was significantly higher in the FA group than in the control group ( p = 0.0302). The morphokinetics during cleavage, morula, and blastocyst stages were comparable between the groups. The outgrowth was significantly increased in the FA group ( p = 0.0438). Clinical study: The rates of implantation, clinical pregnancy, and ongoing pregnancy after SVCTs were significantly increased in the FA group ( p = 0.0223–0.0281). Conclusions Fatty acid‐supplemented warming solutions effectively improve embryo development to the blastocyst stage and pregnancy outcomes after SVCTs.

show abstract

“…Pregnancy outcomes from vitrified oocytes vary from the quality of oocytes before freezing and the operators, who have different knowledge levels and use different technologies [7,8]. In addition, several studies have reported that vitrification of mammalian oocytes can induce the generation of abnormal reactive oxygen species (ROS), accumulation of histone γ-H2AX, and increase apoptosis rate, as a result of lowing developmental potential of both early embryo and fetal [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Beyond Survival Effects of Vitrification-Warming on Epigenetic Modification and Maternal Transcripts of Oocytes

Liu¹,

Chen²,

Chian³

2023

Embryology Update

View full text Add to dashboard Cite

Oocyte vitrification-warming is a worldwide used technique for human fertility preservation. The question of whether the potential risk is associated with this specific procedure remains unresolved. As a fundamental factor of development, oocytes play an important role in early embryonic development, including epigenetic reprogramming and maternal-to-zygotic transition (MZT), that can develop to term. Vitrification, as a significant stressor, appears to have a significant impact on epigenetic modifiers and maternal transcripts of the oocyte, which ultimately results in lower developmental potential. Due to the rapidly evolving single-cell multi-omics sequencing, there have been many advances in this field. We will discuss recent progress in the impact of oocyte vitrification on epigenetic modification and maternal transcripts in this manuscript, hoping to provide a theoretical basis for the optimization and improvement of vitrification-warming technology.

show abstract

Effects of fatty acid supplementation during vitrification and warming on the developmental competence of mouse, bovine and human oocytes and embryos

Cited by 16 publications

References 53 publications

Time from trophectoderm biopsy to vitrification affects the developmental competence of biopsied blastocysts

Time from trophectoderm biopsy to vitrification affects the developmental competence of biopsied blastocysts

Fatty acid supplementation into warming solutions improves pregnancy outcomes after single vitrified‐warmed cleavage stage embryo transfers

Beyond Survival Effects of Vitrification-Warming on Epigenetic Modification and Maternal Transcripts of Oocytes

Contact Info

Product

Resources

About