Effects of fluconazole based medicated feed on haemato-immunological responses and resistance of Labeo rohita against Saprolegnia parasitica

Saha, Himadri; Pal, A. K.; Sahu, Narottam Prasad; Saha, Ratan K.; Goswami, Pritam

doi:10.1016/j.fsi.2017.09.073

Cited by 16 publications

(11 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result is in compliance with Saha et al. () where the decrease in plasma protein content was observed in rohu fed with fluconazole‐based medicated diets. Some scientists have reported that the decrease in plasma protein may occur due to the haemolysis causing dilution of the plasma volume which results in the reduction in plasma protein content to some extent (Das & Mukherjee, ).…”

Section: Discussionsupporting

confidence: 92%

“…Significantly ( p < 0.05) increasing trend of lysozyme was found in fish fed with higher dose of MCZ‐based medicated diet (T3) as compared to control; this result is in line with the observation of Saha et al. () where increasing trend of lysozyme activity was observed in L. rohita fingerlings fed with fluconazole‐based medicated feed. Jian and Wu () also observed higher lysozyme activity in Jian carp fed with traditional Chinese medicine.…”

Section: Discussionsupporting

confidence: 90%

“…Similar results were observed by Saha et al. () where fluconazole‐based medicated feed fed L. rohita also shown significantly increasing trend of NBT activity. This result is consistent with the data obtained by Dugenci, Arda, and Candan (), who reported that extracellular and intracellular respiratory burst activity and phagocytic activity of leucocytes were enhanced by feeding with aqueous extract of powdered ginger roots in rainbow trout.…”

Section: Discussionsupporting

confidence: 89%

“…Control diet was prepared without the addition of MCZ drug. Feeding was done with the respective test diets at 1% of their body weight to achieve the targeted doses (Saha et al., ). The above feeding rate was selected to maintain the dose of application as well as to make sure that there was no left over experimental feeds.…”

Section: Methodsmentioning

confidence: 99%

“…Lethal dose 50% (LD 50 ) of MCZ for rohu fingerlings was found to be 126.14 mgMCZ kgBW −1 (Singh, Saha, Saha, Sahoo, & Biswal, 2018). Three purified test diets were prepared following Saha, Pal, Sahu, Saha, and Goswami (2017) with the addition of calculated amount of sublethal dose of MCZ (Himedia Laboratories, Mumbai, India) as T1 (6.30 mgMCZ kgBW −1 ), T2 (12.61 mgMCZ kgBW −1 ) and T3 (25.22 mgMCZ kgBW −1 ) by manipulating the cellulose level. Control diet was prepared without the addition of MCZ drug.…”

Section: Experimental Diets and Feeding Ratementioning

confidence: 99%

See 4 more Smart Citations

Effect of miconazole nitrate on immunological response and its preventive efficacy in Labeo rohita fingerlings against oomycetes Saprolegnia parasitica

Singh¹,

Saha²,

Saha³

et al. 2018

Journal of Fish Diseases

Self Cite

View full text Add to dashboard Cite

This study evaluated the effect of sublethal doses of antifungal drug miconazole nitrate (MCZ) on immunological responses and its role as a prophylactic drug against S. parasitica in Labeo rohita fingerlings. Fish were fed with sublethal doses of MCZ, that is, T1-6.30 mgMCZ kgBW , T2-12.61 mgMCZ kgBW and T3-25.22 mgMCZ kgBW , and sampling was done at different time intervals for 240 hr. Immunological parameters viz. lysozyme activity, oxygen radical production and plasma antiprotease activity showed significant enhancement (p < 0.05) in fish fed with T2 and T3 doses. Expression of immune-relevant genes such as TLR-22 and β2-M showed significantly higher expression at 6 hr and 24 hr of sampling in both liver and head kidney. However, these genes showed a downregulation after 120 hr of sampling in both the tissues. Preventive efficacy study showed that single dose of MCZ provides protection against oomycetes up to the fourth day of infection. Significantly higher mortality was observed in control diet-fed fish as compared to fish fed with MCZ medicated diet. Thus, it can be concluded that the MCZ can act as a potent antifungal agent for preventing oomycetes infection as well as to enhance the immune response.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Section: Experimental Diets and Feeding Ratementioning

confidence: 99%

See 3 more Smart Citations