Effects of Genetic Variations on Lipoprotein Metabolism in Cardiovascular Diseases

Abstract

Advances in molecular techniques have shown that genetic factors predispose individuals to cardiovascular diseases (CVD). These techniques have made it possible to identify disease-causing genes, prediction to disease susceptibility and responsiveness to drug interventions. For the purpose of this review, therapeutic intervention (niacin) was conducted in a nonhuman primate model to assess the impact of six coincident single nucleotide polymorphisms (cSNP) identiied in prioritised reverse cholesterol transport (RCT) and high-density lipoprotein (HDL) metabolism genes. Gene expression indings conirmed that these genetic variants may have a direct impact on the RCT pathway and drug intervention (niacin) response.

Proprotein convertase subtilisin/kexin type 9 genetic screening using the vervet (Chlorocebus aethiops) model

“…The closed indoor environment was maintained at a temperature of 24–26°C, 45% humidity, 15–20 air changes/h, and a photoperiod of 12 h as previously reported 26 . The monkeys were fed the same food content as previously reported 28,29 …”

“…The PAXgene Blood RNA Kit (Qiagen) was used to isolate and purify intracellular RNA from whole blood collected in the PAXgene Blood RNA Tubes. The exact procedure previously reported for RNA extraction, purification, cDNA conversion 28,29 was employed. The RT 2 qPCR primer assay (Qiagen), designed for SYBR® Green‐based qPCR detection (Applied Biosystems) was used for PCSK9 (PPQ16344A).…”

“…The PAXgene Blood RNA Kit (Qiagen) was used to isolate and purify intracellular RNA from whole blood collected in the PAXgene Blood RNA Tubes. The exact procedure previously reported for RNA extraction, purification, cDNA conversion 28,29 was employed.…”

Mutational analysis of BFSP1, CRYBB1, GALK1, and GJA8 in captive‐bred vervet monkeys (Chlorocebus aethiops)

“…The PAXgene Blood RNA Kit (Qiagen) was used to isolate and purify intracellular RNA from whole blood collected in the PAXgene Blood RNA Tubes (BRT). The exact procedure previously reported for RNA extraction, purification, and cDNA conversion was employed. The RT2 qPCR primer assays (Qiagen), designed for SYBR ® Green‐based RT‐PCR detection (Applied Biosystems), were used for BFSP1 (PPQ02282A), CRYBB1 (PPH01601A), GALK1 (PPH19366A), and GJA8 (PPQ06065A).…”