Effects of glyphosate and methidathion on the expression of the Dhb, Vtg, Arnt, CYP4 and CYP314 in Daphnia magna

Le, Thai-Hoang; Lim, Eun-Suk; Lee, Sung Kyu; Choi, Young‐Woo; Kim, Yang‐Hoon; Min, Jiho

doi:10.1016/j.chemosphere.2009.12.067

Cited by 57 publications

(21 citation statements)

References 19 publications

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“…Even ecotoxicological testing of the active ingredient glyphosate alone has given highly divergent results in D. magna . Some studies have reported LC 50 values of 13–24 mg/l (FAO 2001) whereas others report values of 234 mg/l (Le et al 2010), 780 mg/l (McAllister and Forbis 1978), 930 mg/l (Forbis and Boudreau 1981) or even above 2000 mg/l (Pereira et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Clone- and age-dependent toxicity of a glyphosate commercial formulation and its active ingredient in Daphnia magna

2012

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Low levels of glyphosate based herbicide induced significant negative effects on the aquatic invertebrate Daphnia magna. Glyphosate herbicides such as brands of Roundup, are known to be toxic to daphnids. However, published findings on acute toxicity show significant discrepancies and variation across several orders of magnitude. To test the acute effects of both glyphosate and a commercial formulation of Roundup (hereafter Roundup), we conducted a series of exposure experiments with different clones and age-classes of D. magna. The results demonstrated EC50 (48) values in the low ppm-range for Roundup as well as for the active ingredient (a.i.) isopropylamine salt of glyphosate (glyphosate IPA) alone. Roundup showed slightly lower acute toxicity than glyphosate IPA alone, i.e. EC50 values of 3.7–10.6 mg a.i./l, as compared to 1.4–7.2 mg a.i./l for glyphosate IPA. However, in chronic toxicity tests spanning the whole life-cycle, Roundup was more toxic. D. magna was exposed to sublethal nominal concentrations of 0.05, 0.15, 0.45, 1.35 and 4.05 mg a.i./l for 55 days. Significant reduction of juvenile size was observed even in the lowest test concentrations of 0.05 mg a.i./l, for both glyphosate and Roundup. At 0.45 mg a.i./l, growth, fecundity and abortion rate was affected, but only in animals exposed to Roundup. At 1.35 and 4.05 mg a.i./l of both glyphosate and Roundup, significant negative effects were seen on most tested parameters, including mortality. D. magna was adversely affected by a near 100 % abortion rate of eggs and embryonic stages at 1.35 mg a.i./l of Roundup. The results indicate that aquatic invertebrate ecology can be adversely affected by relevant ambient concentrations of this major herbicide. We conclude that glyphosate and Roundup toxicity to aquatic invertebrates have been underestimated and that current European Commission and US EPA toxicity classification of these chemicals need to be revised.

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Section: Introductionmentioning

confidence: 99%

Clone- and age-dependent toxicity of a glyphosate commercial formulation and its active ingredient in Daphnia magna

2012

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“…This sublethal concentration, 234 mg/L for glyphosate and 0.044 mg/L for methidathion, was determined by the 24 h acute toxicity test reported in a previous study 9 . The exposure test was performed according to the USEPA manual 2002.…”

Section: Analysis Of Protein Expressionmentioning

confidence: 99%

“…After the exposure tests were conducted using ten org anisms per 300 mL test solution in 500 mL chambers with three replicates. Test conditions were controlled at 20±1°C for 24 h with a photoperiod of 16 h light : 8 h darkness 9 .…”

Section: Analysis Of Protein Expressionmentioning

confidence: 99%

Proteomic analysis of Daphnia magna exposed to glyphosate and methidathion

Ahn

Yoon

et al. 2015

Toxicol. Environ. Health Sci.

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Proteomic analysis was performed to identify pro teins involved in the stress responses of Daphnia magna to glyphosate and methidathion. Lethal con centration of chemicals causing 50% mortality (LC50) determined by acute toxicity assays were used to expose to daphnias from 24 h to 21 days prior to total protein extraction. The proteomic profile of tested organisms was analyzed by the twodimensional electrophoresis method. The average intensity of each spots was then analyzed to determine the dif ferently expressed proteins (DEPs). The DEPs was considered as down or upregulations only if their expression level in control samples are linearly higher than LC50 or lower than LC50, respectively. The res ult showed that there are 22 and 27 DEPs respond ing to the exposure of glyphosate and methidathion, respectively. The expression pattern of established DEPs is helpful to understand the molecular response of D. magna to glyphosate and methidathion, and discover potential candidates as novel biomarkers.

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“…Three times per week the medium for the D. magna culture was renewed with fresh HRW and fed with algae (Selenastrum capricormutrum) and YTC (a mixture of yeast, cerophyll and trout chow) purchased from Aquatic Biosystem Inc. (Colorado, US). This algae was also continuously cultured as described (Le et al 2010a). …”

Section: Culture Conditions Of D Magnamentioning

confidence: 99%

Effect of mixed organic compounds extracted from Rhodobacter sphaeroides on Daphnia magna (water flea)

Lee

Park

et al. 2011

World J Microbiol Biotechnol

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In this study, the potential for Rhodobacter sphaeroides, a purple nonsulfur photosynthetic strain of bacteria, to act as a physiological activation material for water flea, Daphnia magna, was tested under two different culture conditions: i.e. YTC (a 1:1 mixture of yeast: Tetramin: Ceropyl) as a basal diet with Selenastrum capricormutrum, a green microalga, and YTC with physiologically activating compounds (PACs), which were defined as mixed organic compounds produced in R. sphaeroides. The impact of PACs produced in R. sphaeroides was assessed by the viability, growth and reproduction of D. magna until the third generation. Compared to S. capricormutrum, it was found that the number of baby daphnia increased after addition to D. magna of PACs produced from dark-cultured R. sphaeroides. Also, we demonstrated that PACs produced from dark-cultured R. sphaeroides influenced individual D. magna growth parameters, such as body length, survival and number of broods per female. Here, we also studied the effect of enhanced sensitivity of D. magna to toxic materials, i.e. titanium oxide and methidathion, when D. magna were grown in culture using PACs. The data provide strong evidence that R. sphaeroides has the potential to substantially increase the physiological activity of D. magna.

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Effects of glyphosate and methidathion on the expression of the Dhb, Vtg, Arnt, CYP4 and CYP314 in Daphnia magna

Cited by 57 publications

References 19 publications

Clone- and age-dependent toxicity of a glyphosate commercial formulation and its active ingredient in Daphnia magna

Clone- and age-dependent toxicity of a glyphosate commercial formulation and its active ingredient in Daphnia magna

Proteomic analysis of Daphnia magna exposed to glyphosate and methidathion

Effect of mixed organic compounds extracted from Rhodobacter sphaeroides on Daphnia magna (water flea)

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