“…Currently, one of the challenges of ecology and conservation biology is to understand the factors that determine the richness, abundance, and distribution of species in megadiverse regions (Arrington and Winemiller, 2005), due to the crisis of biodiversity loss produced by anthropogenic impacts (Cardinale et al, 2012;Montoya-Ospina, López-Delgado, Hevia, Villa-Navarro, 2020). For the Colombian Orinoquia, most of the studies on fish communities have focused on the surveying (Urbano-Bonilla et al, 2009;Ramírez-Gil et al, 2011;Villa-Navarro et al, 2011;Maldonado-Ocampo et al, 2013;Urbano-Bonilla et al, 2014;Usma et al, 2016;Zamudio et al, 2017;Urbano-Bonilla et al, 2018), and a few studies have focused on fish assemblages, based on habitat structural complexity (Montoya-Ospina et al, 2020;Lasso et al, 2021) and temporality (Ospina-O, Bedoya-Giraldo, Villa-Navarro, 2021). Therefore, the objective of this study was to describe the fish assemblage of the Mitimiti stream and test the hypothesis that they vary at a spatial and temporal level.…”