Effects of Hemicastration on Testes and Testosterone Concentration in Stallions

Jung, Hyun-Key; Lee, Geumhui; Kim, Junyoung; Lee, Jangwon; Yoon, Minjung

doi:10.1016/j.jevs.2020.103166

Cited by 4 publications

(1 citation statement)

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“…Using 400× magnification, 100 randomly chosen round STs were examined. As previously mentioned by Jung et al (2020), the πr 2 formula was used to calculate the circular area (μm 2 ) of the ST cross-section. The area of each ST was determined by calculating the average of three measured radii of a cross-section.…”

Section: Histological Characteristics and Cross-sectional Area Of Sem...mentioning

confidence: 99%

Expression pattern of germ cell markers in cryptorchid stallion testes

Shakeel,

Choi,

Yoon

2024

Reprod Domestic Animals

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Cryptorchidism affects spermatogenesis and testis development, often resulting in stallion subfertility/infertility. This study aims to identify the specific germ cells impacted by cryptorchism in stallions. In a previous study, we found that PGP9.5 and VASA are molecular markers expressed in different germ cells within stallions. Herein, we assessed the heat stress‐induced response of spermatogonial stem cells (SSCs) in the seminiferous tubules (ST) of cryptorchid stallion testes (CST) and normal stallion testes (NST). This goal was accomplished by comparing PGP9.5 and VASA expression patterns through reverse transcription quantitative PCR and immunofluorescence assays. We also compared the cross‐sectional ST area between groups. Six post‐pubertal Thoroughbred unilateral cryptorchid stallions were used. The relative abundance of the mRNA transcripts of PGP9.5 and VASA was significantly upregulated in the NST group than in the CST group. Additionally, the cross‐sectional ST area and localization of PGP9.5 and VASA in germ cells were significantly higher in the NST group than in the CST group. Regarding Leydig cells, PGP9.5 staining was observed in both groups. Spermatogonia, primary spermatocytes and secondary spermatocytes were immunostained with VASA in the NST group, while immunostaining was only observed in spermatogonia in the CST group. These results indicate long‐term exposure to heat stress conditions, such as cryptorchidism, directly impacts germ cell proliferation and differentiation, leading to impaired spermatogenesis and compromised fertility in stallions.

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Section: Histological Characteristics and Cross-sectional Area Of Sem...mentioning

confidence: 99%