Effects of Hexane Root Extract ofFerula hermonisBoiss. on Human Breast and Colon Cancer Cells: AnIn VitroandIn VivoStudy

Abutaha, Nael; Nasr, Fahd A.; Al-Zharani, Mohammed; Alqahtani, Ali S.; Noman, Omar M.; Mubarak, Muhammed; Semlali, Abdelhabib; Wadaan, Muhammad A.

doi:10.1155/2019/3079895

Cited by 18 publications

(6 citation statements)

References 47 publications

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“…One of the Ferula species, F. szowitsiana showed a solid cytotoxic property against MCF-7 cell line (IC50: 61.3 µg/ml) 43 . Additionally, root hexane extracts of F. hermonis showed a dose-dependent cytotoxic effect against MDA-MB-231 (IC50: 18.2 μg/ml) 44 .…”

Section: Resultsmentioning

confidence: 99%

Investigation of composition, antioxidant, antimicrobial and cytotoxic characteristics from Juniperus sabina and Ferula communis extracts

Kavaz

Faraj

2023

Sci Rep

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Plants have been one the most valuable sources of biologically active compounds. This study investigates the chemical composition, as well as the antioxidant, antimicrobial, and cytotoxic activities of methanolic and ethanolic extracts from Juniperus sabina and Ferula communis leaves, grown in Cyprus. Total phenolic and flavonoids content of methanol and ethanol extracts were quantified. Chemical constituents of the leaf extracts were analysed using gas chromatography/mass spectrometry (GC/MS). Mome inositol was the predominant component in the J. Sabina’s extracts. The most dominant component in F. communis ethanolic extract was phytol, while in FCL methanolic extract 1,3,4,5 tetrahydroxycyclohexanecarboxylic acid. Antioxidant activities were evaluated by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging ability. Antioxidant activity results revealed concentration dependent activity for methanolic and ethanolic extracts from the plant leaves. Antibacterial activity of plant extracts was tested against Gram-negative and Gram-positive bacteria using disk diffusion and minimal inhibitory concentration methods. Cytotoxic activity of plant extracts were evaluated on MCF-7 and MDA-MB-231 breast cancer cell lines, where they demonstrated their potential on the viability of both cell lines. The biological activity revealed by plants is due to the bioactive compounds found in the extracts. These bioactive components could be used as anticancer drug candidates.

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Section: Resultsmentioning

confidence: 99%

Investigation of composition, antioxidant, antimicrobial and cytotoxic characteristics from Juniperus sabina and Ferula communis extracts

Kavaz

Faraj

2023

Sci Rep

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“…The IC50 value of siderol, a well-known major constituent of the genus Sideritis, was calculated as 26.4±3.7 µM in DLD-1 cell line (Tomou et al, 2020). The hexane extract of Ferula hermonis root (IC50: 25 µg/mL) was found to have moderate sensitivity on the LoVo cell line (Abutaha et al, 2019). In a different study, cytotoxic activities of the methanol 80% extracts and the hexane, chloroform, and methanol fractions of Ferula szowitsiana, and Ferula hirtella were tested on the HT-29 line.…”

Section: Discussionmentioning

confidence: 99%

Anti-Colorectal Cancer Effects of Medicinal Plants: Euphorbia helioscopia, Ferula elaeochytris, and Sideritis albiflora

Deveci

Tel‐Çayan

Karakurt

et al. 2020

Commagene Journal of Biology

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Phytochemicals, extracts, and mixtures obtained from plants have been offered as an option for cancer treatment and prevention for modern drug discovery in recent years. For this purpose, in this study, anti-colorectal cancer effects of the hexane, acetone, methanol, and water extracts obtained by sequential extraction from Euphorbia helioscopia L., Ferula elaeochytris Korovin, and Sideritis albiflora Hub.-Mor. on DLD-1 cell line were investigated in vitro by using Alamar blue assay. Dosedependent inhibition was detected in the viability of DLD-1 cell line. In all three plants species, E. helioscopia (IC50: 140.83±0.31 µg/mL), F. elaeochytris (IC50: 67.93±0.12 µg/mL), and S. albiflora (IC50: 85.12±0.10 µg/mL) methanol extracts showed higher anti-colorectal effects on DLD-1 cell line compared to other extracts tested for the same species. In addition, the IC50 value of doxorubicin used as a standard was found as 6.10±0.55 µg/mL. With the results obtained, as the first report highlighting in vitro anti-colorectal cancer effects of the studied plant species on DLD-1 cell line, promising marks were obtained from the analysis of the extracts as anti-cancer sources for plant-derived drug applications.

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“…Each primer pair ( Table 1 ) was used separately. The following conditions were used to determine the relative gene expression: reverse transcription was performed at 45°C for 45 minutes as a starting point, initial denaturation at 95°C with 10 min hold, followed by 40 cycles of 95°C for 15 sec, and 60°C for 60 sec [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

Cytotoxic Activity, Cell Cycle Inhibition, and Apoptosis-Inducing Potential of Athyrium hohenackerianum (Lady Fern) with Its Phytochemical Profiling

Elasbali

Al‐Soud

Al‐Oanzi

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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In the present study, we investigated the cytotoxic effects of Athyrium hohenackerianum ethanolic extract (AHEE) on the proliferation of breast, lung, and colon cancer cells. The AHEE was tested for its effect on the progression of the cell cycle, followed by induction of apoptosis determination by flow cytometry. Real-time qRT-PCR was also utilized to observe the initiation of apoptosis. In addition, GC-MS was performed in order to identify the active phytochemicals present in the AHEE. A cytotoxic activity with an IC50 value of 123.90 μg/mL against HCT-116 colon cancer cells was exhibited by AHEE. Following propidium iodide staining, annexin-V/PI, and clonogenic assays, AHEE treatment results in cell arrest in the S phase, causing an increase in the early and late phases of apoptosis and displaying antiproliferative potential, respectively. The morphological alterations were further monitored using acridine orange/ethidium bromide (AO/EB) staining. When compared with the control cells, features of apoptotic cell death, including nuclear fragmentation, in the AHEE-treated cells were noticed. The apoptosis was also confirmed by detecting the increased expression of p53 and caspase-3 along with the downregulation of Bcl-2. GC-MS analysis revealed that trans-linalool oxide, loliolide, phytol, 4,8,12,16-tetramethylheptadecan-4-olide, and gamma-sitosterol were the major phytochemical constituents. Based on these findings, it can be suggested that AHEE causes cellular death via apoptosis, which should be further explored for the identification of active compounds responsible for these observed effects. Therefore, AHEE can be used in the pharmaceutical development of anticancer agents for cancer therapeutics.

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Effects of Hexane Root Extract ofFerula hermonisBoiss. on Human Breast and Colon Cancer Cells: AnIn VitroandIn VivoStudy

Cited by 18 publications

References 47 publications

Investigation of composition, antioxidant, antimicrobial and cytotoxic characteristics from Juniperus sabina and Ferula communis extracts

Investigation of composition, antioxidant, antimicrobial and cytotoxic characteristics from Juniperus sabina and Ferula communis extracts

Anti-Colorectal Cancer Effects of Medicinal Plants: Euphorbia helioscopia, Ferula elaeochytris, and Sideritis albiflora

Cytotoxic Activity, Cell Cycle Inhibition, and Apoptosis-Inducing Potential of Athyrium hohenackerianum (Lady Fern) with Its Phytochemical Profiling

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