Effects of Hierarchical Micro/Nano-Textured Titanium Surface Features on Osteoblast-Specific Gene Expression

Meng, Weiyan; Zhou, Yanmin; Zhang, Yanjing; Cai, Qing; Yang, Li‐Ming; Wang, Baixiang

doi:10.1097/01.id.0000434273.22605.78

Cited by 19 publications

(19 citation statements)

References 31 publications

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“…Nine studies found that specific surfaces induced osteoblast differentiation, expression of osteogenic genes or repression of the miRNAs that downregulate osteogenic genes [46][47][48]50,52,53,[58][59][60], while six articles did not indicate a better surface [49,51,[54][55][56][57]. Two articles documented similar trends in gene expression regardless of implant surface and found that the effect of time influenced gene expression more than the surfaces [49,54].…”

Section: Comparative Analysismentioning

confidence: 89%

“…Publication types of the included articles are expressed in Figure 2. Fifteen of 17 articles were in vitro or in vivo studies [46][47][48][49][50][51][52][53][54][55][56][57][58][59][60]. One was a randomized clinical trial with immune-histochemical analysis [61], and the other was a narrative review [62].…”

Section: Resultsmentioning

confidence: 99%

“…Ionized surfaces induced higher levels of Runx-2, Smad5, and osteocalcin [47]. Electrolytic etched surfaces were associated with higher levels of alkaline phosphatase, osteocalcin, Runx-2, osteopontin, and collagen type Iα1 [48]. Sandblasted and acid-etched (SLA) and modSLA surfaces downregulated miRNAs influencing osteoprogenitor cells [51].…”

Section: Implant Surfacesmentioning

confidence: 99%

“…Seven articles did not provide information on miRNA production [46,[48][49][50]54,59,60]. No articles reported information on chromatin alteration due to DNA methylation or histone modification.…”

Section: In Vivo and In Vitro Studiesmentioning

confidence: 99%

“…Regarding in vitro studies, four studies used human osteoblast like cells (MG-63) for cell culture [48,53,56,57] while the other four used human alveolar stem cells from human donors [51,52,55,59]. Human mesenchymal stem cells [60], dental pulp stem cells [47], rat bone marrow cells [58], and marrow stromal cells obtained from the iliac crest [46] were used in one study each.…”

Section: Cell Populationmentioning

confidence: 99%

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The Role of Epigenetics on Dental Implant Therapy: A Systematic Review

Gianfilippo¹,

Gianfilippo²,

Prato

2017

Epigenomes

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Cell phenotype is influenced by the linear sequence of bases and by epigenetic changes. Despite the huge number of implants placed every year, epigenetic mechanisms controlling peri-implant processes remain unexplored. The purpose of this systematic qualitative review was to investigate the available articles dealing with the relationships between DNA methylations, histone modifications, or micro-RNA (miRNA) production and implant therapy. A large variety of different surfaces were evaluated based on their osteogenic stimulation of osteoblasts. Dental implant treatments like potassium hydroxide (KOH) alkali-etching, electrolytic etching, ionization, functionalization with miRNAs or anti-miRNAs, or osteogenic peptides enhanced osteoblast differentiation and gene activation by regulating miRNA production. Zirconia and anatase coating inhibited the activation of osteogenic genes. Epigenetic changes on peri-implant cells induced by smoking still remain unclear. Due to the heterogeneity of methodologies, a meta-analysis was not possible. Even if it is impossible to define which implant surface was best to genetically stimulate osteogenesis, there is evidence that implant surface features can upregulate or downregulate genes related to osseointegration.

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Section: Comparative Analysismentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Section: Implant Surfacesmentioning

confidence: 99%

Section: In Vivo and In Vitro Studiesmentioning

confidence: 99%

Section: Cell Populationmentioning

confidence: 99%

See 3 more Smart Citations

The Role of Epigenetics on Dental Implant Therapy: A Systematic Review

Gianfilippo¹,

Gianfilippo²,

Prato

2017

Epigenomes

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Early bone response to machined, sandblasting acid etching (SLA) and novel surface‐functionalization (SLAffinity) titanium implants: characterization, biomechanical analysis and histological evaluation in pigs

Chiang

Hsu

Peng

et al. 2015

J Biomedical Materials Res

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The purpose of the present study was to examine early tissue response and osseointegration in the animal model. The surface morphologies of SLAffinity were characterized using scanning electron microscopy and atomic force microscopy. The microstructures were examined by X-ray diffraction, and hardness was measured by nanoindentation. Moreover, the safety and toxicity properties were evaluated using computer-aided programs and cell cytotoxicity assays. In the animal model, implants were installed in the mandibular canine-premolar area of 12 miniature pigs. Each pig received three implants: machine, sandblasted, large grit, acid-etched, and SLAffinity-treated implants. The results showed that surface treatment did affect bone-to-implant contact (BIC) significantly. At 3 weeks, the SLAffinity-treated implants were found to present significantly higher BIC values than the untreated implants. The SLAffinity treatments enhanced osseointegration significantly, especially at early stages of bone tissue healing. As described above, the results of the present study demonstrate that the SLAffinity treatment is a reliable surface modification method.

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Effects of the micro–nano surface topography of titanium alloy on the biological responses of osteoblast

Yin

Zhang

Cai

et al. 2016

J Biomedical Materials Res

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In clinical applications, osseointegration is essential for the long-term stability of dental implants. Inspired by the hierarchical structure of natural bone, we applied the electrochemical etching (EC) technique to form a micro-nano structure on a titanium alloy (Ti6Al4V) substrate, called EC surface. Sand blasting and acid etching (SLA) and machined (M) methods were employed to generate micro and smooth textures, respectively, as the control groups. The surface topographies of the three substrates were characterized using scanning electron microscopy (SEM). Then, human osteoblast-like cells (MG63) were cultured on substrates, and adhesion, proliferation, morphology, alkaline phosphatase activity (ALP), and gene expression levels of Runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), osteopontin (OPN), and type I collagen (COLIA 1) were analyzed. MG63 cells cultured on the EC Ti alloy substrates displayed better cell adhesion, significant proliferation, and a higher production level of ALP, gene expressions of RUNX2, OCN, OPN and COLIA 1 (p < 0.01 or p < 0.05) compared with those of SLA and M substrates. These results indicate that the micro-nano structure fabricated by electrochemical etching method is beneficial for the biological functions of MG63 cells and may be a promising candidate in dental implants. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 757-769, 2017.

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Effects of Hierarchical Micro/Nano-Textured Titanium Surface Features on Osteoblast-Specific Gene Expression

Cited by 19 publications

References 31 publications

The Role of Epigenetics on Dental Implant Therapy: A Systematic Review

The Role of Epigenetics on Dental Implant Therapy: A Systematic Review

Early bone response to machined, sandblasting acid etching (SLA) and novel surface‐functionalization (SLAffinity) titanium implants: characterization, biomechanical analysis and histological evaluation in pigs

Effects of the micro–nano surface topography of titanium alloy on the biological responses of osteoblast

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