Effects of hydrostatic pressure, agitation and CO₂ stress on Phytophthora nicotianae zoospore survival

Abstract

Exposure to CO(2) killed P. nicotianae zoospores in water. Neither pressure nor agitation had an effect on zoospore viability or infectivity. Based on results of this study, the authors designed a recycling CO(2) water treatment system that is currently under evaluation.

Simultaneous quantification of sporangia and zoospores in a biotrophic oomycete with an automatic particle analyzer: Disentangling dispersal and infection potentials

“…), the production (Eye et al, 1978;Wilkinson et al, 2001;Dalio et al, 2014), mobility (Pezet et al, 2004), viability (Galiana et al, 2005;Ahonsi et al, 2010) and germination rate (Matheron and Porchas, 2000;Galiana et al, 2005) of zoospores have been studied. Zoospore production has been reported as a tool to standardize inoculum suspensions before infection experiments (Stein and Kirk, 2003) and to evaluate the fitness of isolates (Porter et al, 2007).…”

Benefits of photo-Fenton at low concentrations for solar disinfection of distilled water. A case study: Phytophthora capsici

“…Therefore, the experiments were done with encysted zoospores as no difference in terms of viability between swimming and encysted zoospores were observed before (data not shown). Moreover, similar results for both types of zoospores have been reported in the literature [28]. Malt agar was used for P. capsici colonies counting.…”

Zoospore Production Biology of Pythiaceous Plant Pathogens

“…Each beaker was gently stirred for an even distribution of zoospores in suspension, and a 0.5-ml aliquot was taken and placed into a 2-ml microcentrifuge tube. The tubes were vortexed (Fisher Scientific Touch Mixer Model 231) to encyst the spores (Ahonsi et al 2010). Ten microlitres was taken and placed into chambers of a haemocytometer (Hausser Scientific, Horsham, PA, USA) where zoospores were counted with the aid of a light microscope (Nikon Eclipse E200; Nikon, Inc., Melville, NY, USA).…”