Effects of IL-4 and IL-13 on adenosine receptor expression and responsiveness of the human mast cell line 1

Versluis, Mieke; Postma, Dirkje S.; Timens, Wim; Hylkema, Machteld N.

doi:10.1016/j.intimp.2008.02.001

Cited by 8 publications

(7 citation statements)

References 36 publications

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“…A 2A adenosine receptors have consistently been shown to transmit anti-inflammatory signals to immune cells, including mast cells [20], [39], [40]. These observations of changes in adenosine receptor expression following IL-4 incubation differ from a previously published study by Versluis et al with HMC-1 cells incubated with IL4/IL-13 in several ways [41]. First, we used primary cultures of human mast cell rather than the HMC-1 cell line, which lacks many cardinal features of mast cells important in the pathogenesis of asthma (e.g.…”

Section: Discussioncontrasting

confidence: 58%

IL-4 Amplifies the Pro-Inflammatory Effect of Adenosine in Human Mast Cells by Changing Expression Levels of Adenosine Receptors

et al. 2011

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Adenosine inhalation produces immediate bronchoconstriction in asthmatics but not in normal subjects. The bronchospastic effect of adenosine is largely mediated through adenosine-induced mast cell activation, the mechanism of which is poorly understood due to limitations in culturing human primary mast cells. Here, we show that human umbilical cord blood -derived mast cells incubated with the Th2 cytokine IL-4 develop increased sensitivity to adenosine. Potentiation of anti-IgE- induced and calcium ionophore/PMA-induced degranulation was augmented in mast cells cultured with IL-4, and this effect was reduced or abolished by pre-treatment with A2BsiRNA and selective A2B receptor antagonists, respectively. IL-4 incubation resulted in the increased expression of A2B and reduced expression of A2A adenosine receptors on human mast cells. These results suggest that Th2 cytokines in the asthmatic lung may alter adenosine receptor expression on airway mast cells to promote increased responsiveness to adenosine.

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Section: Discussioncontrasting

confidence: 58%

IL-4 Amplifies the Pro-Inflammatory Effect of Adenosine in Human Mast Cells by Changing Expression Levels of Adenosine Receptors

et al. 2011

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“…The HMC-1 human mast cells were used as a positive control, since these cells are sensitive to IL-13 and may express subunits of the IL-13 receptor (22). A high level of IL13Rα2 transcripts was detected in ARCaP M (Figure 3A), while a reduced level was found in ARCaP E cells, agreeing with the western blotting results (Figure 1).…”

Section: Resultsmentioning

confidence: 99%

Differential expression of the α2 chain of the interleukin‐13 receptor in metastatic human prostate cancer ARCaP_M cells.

Nelson

et al. 2010

The Prostate

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Background The α2 chain of the interleukin-13 receptor (IL13Rα2) is a high affinity receptor and a candidate target for cytotoxic killing of cancer cells. Availability of a human prostate cancer cell line with high level of IL13Rα2 expression will facilitate the development of therapeutic modalities. Methods ARCaPE and ARCaPM human prostate cancer cell lines were subjected to comparative analyses of gene expression. Expression of the IL13Rα2 protein was confirmed by western blotting and immunostaining. IL13Rα2 proteins in xenograft tumors and clinical human prostate cancer specimens were detected by specific antibodies. LNCaP prostate cancer cells stably transfected with IL13Rα2 were examined for accelerated growth in athymic mice. Results We found that IL13Rα2 proteins could be detected in both the ARCaPE and ARCaPM cells, but the expression level in ARCaPM was more than 17-folds higher than in ARCaPE cells. Importantly, the ARCaP lineage represented the only human prostate cancer cell line that expresses IL13Rα2 proteins at the level detectable by western blotting. Expression of IL13Rα2 was accompanied by resistance to the anti-tumor activity of interleukin-13 (IL-13). ARCaP cells were found to be insensitive to growth inhibition upon IL-13 treatment, while overexpression of IL13Rα2 in LNCaP cells promoted intratibial tumor growth in athymic mice. Conclusions Differential IL13Rα2 expression may account for the high tumorigenic and metastatic potential of ARCaPM cells. The unique expression of IL13Rα2 makes ARCaP lineage an attractive model for evaluating the targeting efficacy of therapeutic agents based on IL13Rα2 protein expression.

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“…The levels of IL-4 and IL-13 in the Th2 cells and in serum are associated with asthmatic symptoms that are the most important phenotype markers in peripheral lung tissues, as well as in serum, during hypersensitivity events (3,10,12). Other expressions, such as T-bet, eotaxin, IgE, ß-hexosaminidase and histamine will also affect asthmatic symptoms in vivo.…”

Section: Resultsmentioning

confidence: 99%

“…Interleukin-4 (IL-4) and interleukin-13 (IL-13) are the main immunomodulators to induce the autoimmune responses that are excessively secreted by stimulated Th2 cells, moreover, following that the immunoglobulin E (IgE) and histamine produced by nascent mast cells increases their levels around the bronchial epithelial cells, although these events make the symptoms acute (3). Asthma, atopic dermatitis, and rhinitis that are known to go through a similar signaling process on hyper-responsive symptoms around the epithelial tissues have similar molecular events (10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

Anti-asthmatic activities in mycelial extract and culture filtrate of Cordyceps sphecocephala J201

Heo

Nam²,

Nam³

et al. 2010

Int J Mol Med

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Abstract. This study investigated the effects of mycelial extract and culture filtrate of Cordyceps sphecocephala J201 on airway hyper-responsiveness, pulmonary immune cell infiltration, and Th2 cytokine expression in animal models of asthma. After Concanavalin A (Con A) activation of mouse primary spleen cells, the IL-4 and IL-13 cytokine expression were significantly decreased in the presence of the mycelial extract and culture filtrate of Cordyceps sphecocephala J201. The asthma model was induced by sensitization to ovalbumin by intraperitoneal (i.p.) injection treatment in mice. The Cordyceps sphecocephala J201 mycelial extract was injected in order to assess the effects of anti-asthmatic activity by comparing lung cell infiltration in ovalbumin-induced asthmatic mice. The results revealed that the increased IL-4, IL-13 and IL-25 expression were controlled by the mycelial extract and culture filtrate of Cordyceps sphecocephala J201, indicating that the extracts reduced the undesirable immune responses and/or cytokine expression exhibited in asthma.

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Effects of IL-4 and IL-13 on adenosine receptor expression and responsiveness of the human mast cell line 1

Cited by 8 publications

References 36 publications

IL-4 Amplifies the Pro-Inflammatory Effect of Adenosine in Human Mast Cells by Changing Expression Levels of Adenosine Receptors

IL-4 Amplifies the Pro-Inflammatory Effect of Adenosine in Human Mast Cells by Changing Expression Levels of Adenosine Receptors

Differential expression of the α2 chain of the interleukin‐13 receptor in metastatic human prostate cancer ARCaP_M cells.

Anti-asthmatic activities in mycelial extract and culture filtrate of Cordyceps sphecocephala J201

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Effects of IL-4 and IL-13 on adenosine receptor expression and responsiveness of the human mast cell line 1

Cited by 8 publications

References 36 publications

IL-4 Amplifies the Pro-Inflammatory Effect of Adenosine in Human Mast Cells by Changing Expression Levels of Adenosine Receptors

IL-4 Amplifies the Pro-Inflammatory Effect of Adenosine in Human Mast Cells by Changing Expression Levels of Adenosine Receptors

Differential expression of the α2 chain of the interleukin‐13 receptor in metastatic human prostate cancer ARCaPM cells.

Anti-asthmatic activities in mycelial extract and culture filtrate of Cordyceps sphecocephala J201

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Differential expression of the α2 chain of the interleukin‐13 receptor in metastatic human prostate cancer ARCaP_M cells.