Effects of immediate or delayed addition of platelet additive solution on the in vitro quality of apheresis platelets

Abstract

Adding PAS immediately or 2 hours after collection does not result in different in vitro quality of PLTs stored up to 7 days. The good recovery of clotting factors with no signs of activation indicates a good quality of CP.

Evaluation of store lesion in platelet obtained by apheresis compared to platelet derived from whole blood and its impact on the in vitro functionality

“…In the same way, Gyöngyössy et al founded that platelet concentrates resuspended in additive solution with glucose showed similar characteristics in activation (CD62P), morphology, pH glucose metabolism and swirl, when they were compared to platelets in plasma [38]. Ringwald et al [39] suggested that adding PAS immediately or 2 hours after the collection does not affect the in vitro activation and quality of stored platelets up to 7 days. Anyway, we observed significant differences in CD62P between platelets in PAS with respect to PAP only at day 5.…”

Wissenschaftliche Erläuterungen zur Stellungnahme „Bewertung von Apherese- und Pool-Thrombozytenkonzentraten“ des AK Blut vom 31.03.2015

“…Es ist möglich, auch ATK plasmareduziert in additiver Lösung herzustellen [64][65][66][67][68][69] [90]. In diesen Studien wurden fast ausschließlich ATK verwendet [83,87,95].…”

Standardization of CD62P measurement: results of an international comparative study

“…Even within recent publications, assessment of CD62P expression levels at or just past expiry (5-or 6-day stored) in platelet components produced by the same method (platelet-rich plasma) varies between 15% [9] and 47% [3]. These differences lead to difficulties in collating results from multicentre studies [10,11] and further hamper efforts to determine the clinical relevance of CD62P expression and to further develop CD62P measurement as a meaningful assessment of platelet quality. This issue was first investigated by the Biomedical Excellence for Safer Transfusion (BEST) Collaborative more than a decade ago.…”