Effects of including cationic proteins from cod milt in the feed to Atlantic cod (Gadus morhua) fry during a challenge trial with Vibrio anguillarum

Stimulation of type I IFN activity in Atlantic salmon (Salmo salar L.) leukocytes: Synergistic effects of cationic proteins and CpG ODN

“…Simultaneously, levels of Mx transcripts were significantly elevated. Cod milt histones have earlier been shown to induce non-specific immune mechanisms in fish [22,23]. Cationic liposomes used as a delivery system for pDNA increased cytokine production in a murine cell line alone [47], and injection of pArg alone potentiated specific and lasting immunization of mouse against a peptide antigen [48].…”

“…A chromatin fraction was prepared from the milt as described by Pedersen et al [23], and cationic proteins were extracted from this fraction by addition of sulphuric acid to 0.2 M, on ice [24]. The remaining sediment was re-extracted by five volumes of 0.2 M H 2 SO 4 on ice.…”

The use of immunostimulants in fish larval aquaculture

“…However, the majority of studies concern delivery of immunostimulant in the diet [56,103,109] and only Kennedy et al [108] have opted for an environmental approach to the delivery of immunostimulants.…”

Viral and bacterial diseases of Atlantic cod Gadus morhua, their prophylaxis and treatment: a review

“…In Atlantic cod fry, oral administration of immunostimulants (fish protein hydrolysate or cationic proteins from cod milt) or probiotics (Carnobacterium divergens isolated from the intestines of Atlantic salmon or Atlantic cod) has resulted in some reduction of mortality after bath challenge with Listonella anguillarum (Gildberg & Mikkelsen 1997, Pedersen et al 2004). Furthermore, cod larvae bath treated with bacterial lipopolysaccharide (LPS) or a potentially probiotic bacterium (Vibrio sp., isolated from cod) displayed significantly improved survival, but no protection was observed after experimental infection with Aeromonas salmonicida ssp.…”