Effects of Lactobacillus rhamnosus GG on the serotonergic pathway in a gliadin-induced enteropathy animal model

Orlando, Antonella; Clemente, Caterina; D’Attoma, Benedetta; Russo, Francesco

doi:10.1016/j.jff.2020.104077

Cited by 5 publications

(4 citation statements)

References 47 publications

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“…5-HTR4 is an important therapeutic target for treatment of chronic constipation ( Hoffman et al., 2012b ; Gwynne and Bornstein, 2019 ). After different modalities of treatment in healthy or gastrointestinal function disturbed rodent models, the secretion of 5-HT increases, and the expression of 5-HTR4 receptor is upregulated, suggesting that 5-HT and 5-HTR4 receptors may be correlated ( Orlando et al., 2020 ; Yaghoubfar et al., 2020 ; Zhu et al., 2020 ). Given the effect of EcN-5-HT on the 5-HT level in colon, we investigated the expression of 5-HTR4 gene.…”

Section: Resultsmentioning

confidence: 99%

Engineered 5-HT producing gut probiotic improves gastrointestinal motility and behavior disorder

Li¹,

Li²,

Luo³

et al. 2022

Front. Cell. Infect. Microbiol.

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Slow transit constipation is an intractable constipation with unknown aetiology and uncertain pathogenesis. The gut microbiota maintains a symbiotic relationship with the host and has an impact on host metabolism. Previous studies have reported that some gut microbes have the ability to produce 5-hydroxytryptamine (5-HT), an important neurotransmitter. However, there are scarce data exploiting the effects of gut microbiota-derived 5-HT in constipation-related disease. We genetically engineered the probiotic Escherichia coli Nissle 1917 (EcN-5-HT) for synthesizing 5-HT in situ. The ability of EcN-5-HT to secrete 5-HT in vitro and in vivo was confirmed. Then, we examined the effects of EcN-5-HT on intestinal motility in a loperamide-induced constipation mouse model. After two weeks of EcN-5-HT oral gavage, the constipation-related symptoms were relieved and gastrointestinal motility were enhanced. Meanwhile, administration of EcN-5-HT alleviated the constipation related depressive-like behaviors. We also observed improved microbiota composition during EcN-5-HT treatment. This work suggests that gut microbiota-derived 5-HT might promise a potential therapeutic strategy for constipation and related behavioral disorders.

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Section: Resultsmentioning

confidence: 99%

Engineered 5-HT producing gut probiotic improves gastrointestinal motility and behavior disorder

Li¹,

Li²,

Luo³

et al. 2022

Front. Cell. Infect. Microbiol.

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“…Therefore, transporters play a very important role in the transmission of neurotransmitters. SERT is a monoamine transporter,which can terminate 5-HT transmission through rapid presynaptic uptake ( Orlando et al, 2020 ). The fuction of SERT may be related to several different mechanisms, such as the increase of expression of SERT ( Li et al, 2019 ) and the change of SERT conformation ( Plenge et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Phenotype-Based HPLC-Q-TOF-MS/MS Coupled With Zebrafish Behavior Trajectory Analysis System for the Identification of the Antidepressant Components in Methanol Extract of Anshen Buxin Six Pills

et al. 2021

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Phenotype screening has become an important tool for the discovery of active components in traditional Chinese medicine. Anshen Buxin Six Pills (ASBX) are a traditional Mongolian medicine used for the treatment of neurosis in clinical settings. However, its antidepressant components have not been explicitly identified and studied. Here, the antidepressant effect of ASBX was evaluated in adult zebrafish. High performance liquid chromatography-mass spectrometry (HPLC-Q-TOF-MS/MS) was combined with zebrafish behavior trajectory analysis to screen and identify the antidepressant-active extract fraction and active components of ASBX. Finally, the antidepressant effect of the active ingredients were verified by the behavior, pathology, biochemical indices and protein level of adult fish. The novel tank driving test (NTDT) showed that ASBX can effectively improve the depressive effect of reserpine on zebrafish. Petroleum ether and dichloromethane extracts of ASBX were screened as antidepressant active extracts. Costunolide (COS) and dehydrocostus lactone (DHE) were screened as the active components of ASBX. COS had been shown to significantly improve the depressive behavior, nerve injury and neurotransmitter levels (5-hydroxytryptamine (5-HT) and norepinephrine (NE)) of zebrafish by inhibiting the high expression of serotonin transporter and norepinephrine transporter induced by reserpine suggesting the antidepressant effect of COS may be related to its effect on 5-HT and NE pathways. This study provided a phenotype based screening method for antidepressant components of traditional Chinese medicines, so as to realize the separation, identification and activity screening of components at the same time.

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“…and Lactobacillus spp. , have positively alleviated ulcerative colitis by regulating inflammatory agents and protecting the colonic mucosa from injury [ 60 ]. In addition, Lactobacillus fermentum CECT5716 , Lactobacillus salivarius CECT5713 , Escherichia coli Nissle 1917 , and Saccharomyces boulardii CNCMI-745 can also reduce inflammatory cytokines (TNF-α and IL-1β) and increase anti-inflammatory IL-10.…”

Section: Probiotics and Gastrointestinal Dysfunctionsmentioning

confidence: 99%

Probiotic Supplements: Their Strategies in the Therapeutic and Prophylactic of Human Life-Threatening Diseases

Youssef

Ahmed

Zongo

et al. 2021

IJMS

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Chronic diseases and viral infections have threatened human life over the ages and constitute the main reason for increasing death globally. The rising burden of these diseases extends to negatively affecting the economy and trading globally, as well as daily life, which requires inexpensive, novel, and safe therapeutics. Therefore, scientists have paid close attention to probiotics as safe remedies to combat these morbidities owing to their health benefits and biotherapeutic effects. Probiotics have been broadly adopted as functional foods, nutraceuticals, and food supplements to improve human health and prevent some morbidity. Intriguingly, recent research indicates that probiotics are a promising solution for treating and prophylactic against certain dangerous diseases. Probiotics could also be associated with their essential role in animating the immune system to fight COVID-19 infection. This comprehensive review concentrates on the newest literature on probiotics and their metabolism in treating life-threatening diseases, including immune disorders, pathogens, inflammatory and allergic diseases, cancer, cardiovascular disease, gastrointestinal dysfunctions, and COVID-19 infection. The recent information in this report will particularly furnish a platform for emerging novel probiotics-based therapeutics as cheap and safe, encouraging researchers and stakeholders to develop innovative treatments based on probiotics to prevent and treat chronic and viral diseases.

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Effects of Lactobacillus rhamnosus GG on the serotonergic pathway in a gliadin-induced enteropathy animal model

Cited by 5 publications

References 47 publications

Engineered 5-HT producing gut probiotic improves gastrointestinal motility and behavior disorder

Engineered 5-HT producing gut probiotic improves gastrointestinal motility and behavior disorder

Phenotype-Based HPLC-Q-TOF-MS/MS Coupled With Zebrafish Behavior Trajectory Analysis System for the Identification of the Antidepressant Components in Methanol Extract of Anshen Buxin Six Pills

Probiotic Supplements: Their Strategies in the Therapeutic and Prophylactic of Human Life-Threatening Diseases

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