Effects of light emitting diode irradiation on neural differentiation of human umbilical cord-derived mesenchymal cells

Dehghani-Soltani, Samereh; Shojaee, Mohammad; Jalalkamali, Mahshid; Babaee, Abdolreza; Nematollahi-Mahani, Seyed Noureddin

doi:10.1038/s41598-017-10655-w

Cited by 16 publications

(7 citation statements)

References 49 publications

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“…For assessing astrocytic reactivity, we used mouse monoclonal anti-GFAP (Glial Fibrillary Acidic Protein) antibody (Dako A/S Denmark, 1:400) ( Dehghani-Soltani, Shojaee, Jalalkamali, Babaee, & Nematollahi-Mahani, 2017 ). For this purpose, after deparaffinization in a microwave oven with 65°C and rinsing of sections in xylene (5 min), they washed with PBS and were put in the 10 mM citrate buffer for 1 h (temperature of 90°C and pH 6).…”

Section: Methodsmentioning

confidence: 99%

Hippocampal Astrocyte Response to Melatonin Following Neural Damage Induction in Rats

Babaee

Eftekhar-Vaghefi

Asadi-Shekaari

et al. 2021

BCN

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Introduction: Brain injury induces an almost immediate response from glial cells, especially astrocytes. Activation of astrocytes leads to the production of inflammatory cytokines and reactive oxygen species that may result in secondary neuronal damage. Melatonin is an anti-inflammatory and antioxidant agent, and it has been reported to exert neuroprotection through the prevention of neuronal death in several models of central nervous system injury. This study aimed to investigate the effect of melatonin on astrocyte activation induced by Traumatic Brain Injury (TBI) in rat hippocampus and dentate gyrus. Methods: Animals were randomly divided into 5 groups; Sham group, TBI group, vehicle group, and melatonin‐treated TBI groups (TBI+Mel5, TBI+Mel20). Immunohistochemical method (GFAP marker) and TUNEL assay were used to evaluate astrocyte reactivity and neuronal death, respectively. Results: The results demonstrated that the astrocyte number was reduced significantly in melatonin‐treated groups compared to the vehicle group. Additionally, based on TUNEL results, melatonin administration noticeably reduced the number of apoptotic neurons in the rat hippocampus and dentate gyrus. Conclusion: In general, our findings suggest that melatonin treatment after brain injury reduces astrocyte reactivity as well as neuronal cell apoptosis in rat hippocampus and dentate gyrus.

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Section: Methodsmentioning

confidence: 99%

Hippocampal Astrocyte Response to Melatonin Following Neural Damage Induction in Rats

Babaee

Eftekhar-Vaghefi

Asadi-Shekaari

et al. 2021

BCN

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“…Expression of late and early chondrogenic and osteogenic specific markers were surveyed by immuno-histochemistry (IHC). Antibodies against alkaline phosphatase (ALP) from Abcam company (Cat# ab65834) and osteocalcin (OCN) from Millipore company (Cat# ab10911), as osteogenesis markers, and collagen type 2 (COLL2) from Abcam company (Cat# ab34712) and SRY-box transcription factor 9 (SOX9) from Abcam company (Cat# ab3697), as chondrogenic markers, were purchased from Abcam and Millipore companies [ 22 , 33 ]. In IHC cell’s nuclei were stained by hematoxyline.…”

Section: Methodsmentioning

confidence: 99%

Effects of maternal consumption of morphine on rat skeletal system development

Saeidinezhad

Razban

Safizadeh

et al. 2021

BMC Musculoskelet Disord

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Background Opioid abuse is among the most ubiquitous issues world-wide, and when it happens in mothers, it puts them at risk of diseases that can be transferred to the next generation. Previous studies have indicated that morphine addiction during pregnancy could inhibit development in rat embryos and infants. The present study focused on the effects of maternal consumption of morphine on rat skeletal system development and also investigate the molecular pathway of chondrogenesis and osteogenesis of infants from control and addicted rat groups. Methods Thirty-two female rats were randomly assigned to four groups. The groups consisted of one- and seven-day-old female infants which were born of morphine-dependent mothers and a control group for each of them. Experimental groups received oral morphine at the final dose of 0.4 mg/ml/day. Withdrawal signs were confirmation of morphine dependency. Female rats were crossed with male rats and coupling time was recorded. Fixed bones of all groups were processed and then stained by hematoxyline-eosin method. Thickness and cell number of proximal and distal growth plate of bones were measured. The cartilage and bone cells were stained by alcian blue/alizarin red method. Additionally, the gene expression of alkaline phosphatase, osteocalcin, and COLL2 and SOX9 gene expression were studied immuno-histochemically. Results Unfavorable effects of morphine on histological measurements were observed in one-day and seven-day infants, with more effects on seven-day infants. The thickness and cell number of the proximal and distal growth plate of morphine-dependent rat offsprings were reduced significantly. Furthermore, morphine reduced growth of primary and secondary ossification centers, and thus, longitudinal bone growth was reduced. Moreover, a decrease in the alkaline phosphatase, osteocalcin, COLL2 and SOX9 gene expression, and the number of stained cells was observed. More adverse effects of morphine in seven-day infants compared to one-day infants which showed the time dependent of morphine to the time length of administration. Conclusion Histochemistry and immunohistochemistry findings on cartilage and bone matrix formation, as well as protein expression of chondrogenic and osteogenic markers suggest that morphine dependence in pregnant mothers may impair intra-cartilaginous osteogenesis in post-natal rats.

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“…A handmade LED device with green light emission was used in the LED exposure groups (LED, MEL+LED, SC+LED and LED+SC+MEL). Twenty-four hr later the medium was refreshed and the samples were once irradiated for 10 minutes at radiation energy of 1.5 J/cm 2 , and 530 nm wavelength 31 . All the exposure procedures were carried out inside a 37ºC CO 2 incubator.…”

Section: Led Irradiationmentioning

confidence: 99%

Three-Dimensional Scaffold, Light Emitting Diode (LED) And Melatonin Effects on Cell Proliferation and Testosterone Secretion in TM3 Leydig Cells

Alavi

Shojaei²,

Haghpanah

et al. 2021

Preprint

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Different effects of fibrin scaffold, green LED and melatonin on cell proliferation and differentiation have received extensive attentions. Testosterone, the most important sex hormone is secreted by Leydig cells to regulate spermatogenesis and many other phenomena in the body organs. The aim of the present study was to evaluate the proliferation of Leydig cells and their testosterone secretion under the influence of three-dimensional scaffold culture, green light irradiation and melatonin. The experimental groups, were TM3 cells incorporated in 3D scaffold culture, exposed to green light and also exposed to melatonin individually, in pairs and three factors together. Cell proliferation and testosterone secretion were measured after 72 hr. Cell cycle genes including PCNA, CYCLIND1, CDC2 and CDKN1B and, testosterone related genes; GATA4 and RORα were also examined. In general, three-dimensional scaffold increased Leydig cells proliferation, testosterone secretion and the expression of the related genes. On the other hand, melatonin decreased cell proliferation and testosterone synthesis. Green light did not significantly change the results but slightly decreased cell proliferation and testosterone synthesis. Combination of green light with melatonin significantly reduced the outcome while, light with scaffold increased the results. Application of light + scaffold + melatonin covered the decreasing effect of melatonin.

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Effects of light emitting diode irradiation on neural differentiation of human umbilical cord-derived mesenchymal cells

Cited by 16 publications

References 49 publications

Hippocampal Astrocyte Response to Melatonin Following Neural Damage Induction in Rats

Hippocampal Astrocyte Response to Melatonin Following Neural Damage Induction in Rats

Effects of maternal consumption of morphine on rat skeletal system development

Three-Dimensional Scaffold, Light Emitting Diode (LED) And Melatonin Effects on Cell Proliferation and Testosterone Secretion in TM3 Leydig Cells

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