Effects of long non-coding RNA uc.48+ on pain transmission in trigeminal neuralgia

Xiong, Wei; Tan, Mengxi; Tong, Zhoujie; Yin, Chuanqiang; He, Laichang; Liu, Lijuan; Shen, Yulin; Guan, Shu; Ge, Huixiang; Li, Guodong; Liang, Shangdong; Gao, Yun

doi:10.1016/j.brainresbull.2019.02.009

Cited by 28 publications

(31 citation statements)

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“…The P2X3 receptor, belongs to the P2X receptor family (P2X1-7), plays a crucial role in pain transmission at peripheral sensory neurons [7]. Recently, Xiong et al [8] also found that lncRNA uc.48 + siRNA significantly reversed the reduced MWT in the chronic constriction injury (CCI) of the infraorbital nerve (ION) (CCI-ION)-induced TN rats. Evidence also suggested that NONRATT021972 siRNA treatment in type 2 diabetic rats increased the MWT, TWL and the sensory nerve conduction velocity (SNCV) of rat tail nerves [9,10].…”

Section: Introductionmentioning

confidence: 99%

Effects of long non-coding RNA Gm14461 on pain transmission in trigeminal neuralgia

Yan

Zhu

et al. 2020

J Inflamm

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Background: This study aims to investigate the role of long non-coding RNA Gm14461 in regulating pain transmission in trigeminal neuralgia (TN). The mouse TN model was produced by chronic constriction injury of the infraorbital nerve (CCI-ION). The values of mechanical withdrawal threshold (MWT) were measured to assess the nociception of mice at different times after CCI-ION surgery (0, 1, 3, 5, 7, 9, 11, 13, 15 d). The primary mouse trigeminal ganglion neurons (TGNs) were isolated from C57BL/6 J mice and treated with TNF-α to mimic a TN cellular model. The expression of Gm14461, TNF-α, IL-1β, and IL-6 was examined using qRT-PCR. The protein levels of CGRP and P2X3/7 receptor were measured using western blot.Results: Gm14461 expression was increased in trigeminal ganglia (TGs) of TN mice on the operation side. Furthermore, Gm14461 knockdown in TGs increased, whereas Gm14461 overexpression decreased MWT in TN mice. Moreover, Gm14461 knockdown downregulated, whereas Gm14461 overexpression upregulated mRNA levels of TNF-α, IL-1β, and IL-6 and protein levels of CGRP and P2X3/7 receptor in TGs from TN mice. In vitro assay showed that Gm14461 was upregulated by TNF-α, IL-1β, and IL-6. Additionally, Gm14461 knockdown decreased protein levels of CGRP and P2X3/7 receptor in TNF-α-treated TGNs, whereas Gm14461 overexpression exerted the opposite effect.Conclusion: Gm14461 promoted pain transmission (reduced MWT value) in a CCI-ION-induced mouse TN model. The underlying mechanisms might involve the regulation of pro-inflammatory cytokines, CGRP and P2X3/7 receptor.

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Section: Introductionmentioning

confidence: 99%

Effects of long non-coding RNA Gm14461 on pain transmission in trigeminal neuralgia

Yan

Zhu

et al. 2020

J Inflamm

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“…The animals had free access to food and water in a room with a comfortable temperature of 20 °C to 25 °C. The International Association for the Study of Pain (IASP)'s ethical guidelines for pain research in animals were followed 36 .…”

Section: Methodsmentioning

confidence: 99%

“…These rats were randomly divided into 4 groups: sham, TN, sham + palmatine, and TN + palmatine. For the TN group, the right infraorbital nerve was exposed and loosely ligated through a previously described method, but for the sham group, the right infraorbital nerve was exposed but not ligated 36 . For the sham + palmatine group and the TN + palmatine group, 10% palmatine was administered through an intraperitoneal injection of 20 mg/kg a day for 2 weeks.…”

Section: Methodsmentioning

confidence: 99%

“…Evaluation of mechanical allodynia. Mechanical allodynia was measured as described in detail previously in our published paper 36 . The mechanical nociceptive threshold was evaluated using electronic pain meter (BME-404 NO.E5489, Institute of Biomedical Engineering, CAMS & PUMC).…”

Section: Methodsmentioning

confidence: 99%

“…Using a Bio-Rad system, the labelled proteins were then imaged with an enhanced chemiluminescence. The optical density of the target proteins band were calculated using Image-Pro Plus software after normalizing to each β-actin band 36 .…”

Section: Scientific Reports |mentioning

confidence: 99%

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Effects of palmatine on BDNF/TrkB-mediated trigeminal neuralgia

Liu

Yin

et al. 2020

Sci Rep

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Trigeminal neuralgia (TN), a sudden, needle-like pain in the distribution area of the trigeminal nerve, can seriously affect the physical and mental health of patients. In chronic pain conditions including TN, increased levels of brain-derived neurotrophic factor (BDNF) may enhance pain transmission. This study compares the effect of palmatine administration on the expression of BDNF and its receptor TrkB (tropomyosin receptor kinase B) in trigeminal ganglion cells of Sprague-Dawley rats in a shamversus TN model group. Within 14 days of surgery, the mechanical allodynia threshold of the TN group was significantly lower than that of the sham group, while the TN + palmatine group had a higher mechanical pain sensitivity threshold than the TN group (p < 0.05). Real-time quantitative PCR, immunohistochemistry, and immunofluorescence showed that BDNF and TrkB expression in the TN group was higher than that in the sham group, while palmatine treatment could reverse these changes. Western blotting showed that palmatine treatment could reduce the elevated phosphorylation of extracellular signal-regulated kinases 1/2 (ERK1/2) in TN rats. Thus, the BDNF/TrkB pathway may be involved in the pain transmission process of TN, and palmatine treatment may reduce pain transmission by inhibiting the BDNF/TrkB pathway and suppressing ERK1/2 phosphorylation.

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Long non coding RNAs involved in MAPK pathway mechanism mediates diabetic neuropathic pain

Ren

Yang

et al. 2020

Cell Biology International

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Diabetes is the largest global epidemic of the 21st century, and the cost of diabetes and its complications comprise about 12% of global health expenditure. Diabetic neuropathy is the most common complication of diabetes, affecting up to 50% of patients over the course of their disease. Among them, 30%–50% develop neuropathic pain, which has typical symptoms that originate from the toes and progress to foot ulcers and seriously influence quality of life. The pathogenesis of diabetic neuropathic pain (DNP) is complicated and incompletely understood and there is no effective treatment except supportive treatment. Long noncoding RNAs (lncRNAs), a class of noncoding RNAs exceeding 200 nucleotides in length, have been shown to play key roles in fundamental cellular processes, and are considered to be potential targets for treatment. Recent research indicates that lncRNA is involved in the pathogenesis of DNP. Certain overexpressed lncRNAs can enhance the purinergic receptor‐mediated neuropathic pain in peripheral ganglia and inflammatory cytokines are released due to receptors activated by adenosine triphosphate. In recent years, our laboratory also has been exploring the relationship and pathogenesis between lncRNAs and DNP. In this review, we focus on the recent progress in functional lncRNAs associated with DNP and investigate their roles related to respective receptors.

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Effects of long non-coding RNA uc.48+ on pain transmission in trigeminal neuralgia

Cited by 28 publications

References 31 publications

Effects of long non-coding RNA Gm14461 on pain transmission in trigeminal neuralgia

Effects of long non-coding RNA Gm14461 on pain transmission in trigeminal neuralgia

Effects of palmatine on BDNF/TrkB-mediated trigeminal neuralgia

Long non coding RNAs involved in MAPK pathway mechanism mediates diabetic neuropathic pain

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