Effects of Macleaya cordata extract on small intestinal morphology and gastrointestinal microbiota diversity of weaned pigs

Li, Yuan; Xu, Fangming; Tong, Xiao Yan; Chen, Ruonan; Shen, Chunyan; Liang, Tingting; Chu, Qingpo; Zhou, Bo

doi:10.1016/j.livsci.2020.104040

Cited by 13 publications

(12 citation statements)

References 39 publications

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“… 99 , 100 Li et al found that MCE intake significantly increased the relative abundance of Oscillospira in the jejunum of weaned pigs. 43 Cranberry pomace (CBP) is rich in polyphenols, complex carbohydrates, fiber and nutritional minerals. 101 Continuous addition of CBP during the rearing of broiler chickens eventually significantly increased the abundance of Oscillospira in their gut.…”

Section: Factors Affecting the Abundance Of Oscillospiramentioning

confidence: 99%

Oscillospira - a candidate for the next-generation probiotics

et al. 2021

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Oscillospira is a class of organism that often appears in high-throughput sequencing data but has not been purely cultured and is widely present in the animal and human intestines. There is a strong association between variation in Oscillospira abundance and obesity, leanness, and human health. In addition, a growing body of studies has shown that Oscillospira is also implicated in other diseases, such as gallstones and chronic constipation, and has shown some correlation with the positive or negative changes in its course. Sequencing data combined with metabolic profiling indicate that Oscillospira is likely to be a genus capable of producing short-chain fatty acids (SCFAs) such as butyrate, which is an important reference indicator for screening “next-generation probiotics “. Considering the positive effects of Oscillospira in some specific diseases, such as obesity-related metabolic diseases, it has already been characterized as one of the next-generation probiotic candidates and therefore has great potential for development and application in the future food, health care, and biopharmaceutical products.

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Section: Factors Affecting the Abundance Of Oscillospiramentioning

confidence: 99%

Oscillospira - a candidate for the next-generation probiotics

et al. 2021

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“…Intestinal morphology signi cantly changes, including villous atrophy and crypt hyperplasia, which will result in diarrhea and growth retardation in pigs [42]. An increasing villus height/crypt depth ratio is one of the most important indexes of intestinal morphology in evaluating the improvement of intestinal function and enhancement of absorption capacity [43]. A recent study found that dietary supplemented with MCR at 2000 mg/kg improved the ratio of villus height to crypt depth in the jejunum, and increased the villus height and crypt depth in the ileum of weaned piglets.…”

Section: Discussionmentioning

confidence: 99%

Dietary Moutan Cortex Radicis Alters Serum Antioxidant Capacity, Intestinal Immunity and Colonic Microbiota in Weaned Piglets

Bai

Liu

Wang

et al. 2020

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Background: Moutan cortex radicis (MCR), as a common traditional Chinese medicine, has been widely used as antipyretic, antiseptic and anti-inflammatory agent in China. However, few studies have evaluated the positive effects of MCR, as a new feed additives, on alleviating weaning stress and improving intestinal health and microbiom in pigs. This study aimed to investigate the effect of dietary MCR supplementation on serum antioxidant capacity, intestinal morphology, anti-inflammatory mechanism, and microbiota in weaned piglets.Results: Supplemental 2000 mg/kg and 4000 mg/kg MCR increased (P < 0.05) the final body weight, ADG and ADFI of weaned piglets, and 2000 mg/kg MCR diet significantly decreased (P < 0.05) the F/G ratio and increased (P < 0.05) serum catalase activity compared with CON group. Also, the villus height and crypt depth in the ileum and the concentrations of total SCFA, acetic acid, butyric acid and valeric acid in the colonic contents were higher (P < 0.05) in the 2000 mg/kg and 4000 mg/kg MCR diets than CON group. Dietary MCR supplementation at 4000 mg/kg MCR significantly increased (P < 0.05) total antioxidative capability and the crypt depth in the jejunum but decreased (P < 0.05) the mRNA expression levels of Interferon γ, tumor necrosis factor-α, interleukin-1β, inhibiting kappa B kinase β (IKKβ), inhibiting nuclear factor kappa-B (IκBα) and nuclear factor kappa-B (NF-κB) in the jejunum and ileum. Supplemental 8000 mg/kg MCR had the higher total antioxidative capability and catalase activity in the serum but decreased (P < 0.05) the villus height and crypt depth in the jejunum compared with the CON group. MCR addition reduced (P < 0.05) serum malondialdehyde content, and tended to increase the mRNA expression of zonula occludens-1 in the ileum (P = 0.066) compared to the CON group. Microbiota sequencing identified the microbial richness indices (Chao1, ACE, and observed species), the relative abundances of Firmicutes and Lactobacillus were increased (P < 0.05), and the relative abundances of Bacteroides, Parabacteroides, unidentified_Lachnospiraceae and Enterococcus were reduced (P < 0.05) by MCR supplemented. Microbial metabolic phenotypes analysis also showed that the richness of aerobic bacteria and facultative anaerobic bacteria, oxidative stress tolerance, and biofilm forming were significantly increased (P < 0.05), and the richness of anaerobic bacteria and pathogenic potential of gut microbiota were reduced (P < 0.05) by MCR treatment. Conclusions: In antibiotic-free diets, MCR supplementation improved growth performance and serum antioxidant capacity, alleviated intestinal inflammatory by inhibiting IKKβ/IκBα/NF-κB signaling pathway and affecting intestinal microbiota in weaned piglets.

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“…Intestinal morphology significantly changes, including villous atrophy and crypt hyperplasia, which will result in diarrhea and growth retardation in pigs (43). An increasing VH/CD ratio is one of the most important indices of intestinal morphology in evaluating the improvement of intestinal function and enhancement of absorption capacity (44). A recent study found that the dietary supplemented with MCR at 2,000 mg/kg improved the ratio of VH to CD in the jejunum and increased the VH and CD in the ileum of weaned piglets.…”

Section: Mcr Has Potential In Antioxidant and Anti-inflammation Therapy In Weaned Pigletsmentioning

confidence: 99%

Dietary Moutan Cortex Radicis Improves Serum Antioxidant Capacity and Intestinal Immunity and Alters Colonic Microbiota in Weaned Piglets

et al. 2021

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Background:Moutan cortex radicis (MCR), as a common traditional Chinese medicine, has been widely used as an antipyretic, antiseptic, and anti-inflammatory agent in China.Objectives: This study aimed to investigate the effects of dietary MCR supplementation on the antioxidant capacity and intestinal health of the pigs and to explore whether MCR exerts positive effects on intestinal health via regulating nuclear factor kappa-B (NF-κB) signaling pathway and intestinal microbiota.Methods: MCR powder was identified by LC-MS analysis. Selected 32 weaned piglets (21 d of age, 6.37 ± 0.10 kg average BW) were assigned (8 pens/diet, 1 pig/pen) to 4 groups and fed with a corn-soybean basal diet supplemented with 0, 2,000, 4,000, and 8,000 mg/kg MCR for 21 d. After the piglets were sacrificed, antioxidant indices, histomorphology examination, and inflammatory signaling pathway expression were assessed. The 16s RNA sequencing was used to analyze the effects of MCR on the intestinal microbiota structure of piglets.Results: Supplemental 4,000 mg/kg MCR significantly increased (P < 0.05) the average daily weight gain (ADG), average daily feed intake (ADFI), total antioxidative capability, colonic short-chain fatty acids (SCFA) concentrations, and the crypt depth in the jejunum but decreased (P < 0.05) the mRNA expression levels of interferon γ, tumor necrosis factor-α, interleukin-1β, inhibiting kappa-B kinase β (IKKβ), inhibiting nuclear factor kappa-B (IκBα), and NF-κB in the jejunum and ileum. Microbiota sequencing identified that MCR supplementation significantly increased the microbial richness indices (Chao1, ACE, and observed species, P < 0.05) and the relative abundances of Firmicutes and Lactobacillus (P < 0.05), decreased the relative abundances of Bacteroides, Parabacteroides, unidentified_Lachnospiraceae, and Enterococcus (P < 0.05) and had no significant effects on the diversity indices (Shannon and Simpson, P > 0.05). Microbial metabolic phenotypes analysis also showed that the richness of aerobic bacteria and facultative anaerobic bacteria, oxidative stress tolerance, and biofilm forming were significantly increased (P < 0.05), and the richness of anaerobic bacteria and pathogenic potential of gut microbiota were reduced (P < 0.05) by MCR treatment. Regression analysis showed that the optimal MCR supplemental level for growth performance, serum antioxidant capacity, and intestinal health of weaned piglets was 3,420 ~ 4,237 mg/kg.Conclusions: MCR supplementation improved growth performance and serum antioxidant capacity, and alleviated intestinal inflammation by inhibiting the IKKβ/IκBα/NF-κB signaling pathway and affecting intestinal microbiota in weaned piglets.

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Effects of Macleaya cordata extract on small intestinal morphology and gastrointestinal microbiota diversity of weaned pigs

Cited by 13 publications

References 39 publications

Oscillospira - a candidate for the next-generation probiotics

Oscillospira - a candidate for the next-generation probiotics

Dietary Moutan Cortex Radicis Alters Serum Antioxidant Capacity, Intestinal Immunity and Colonic Microbiota in Weaned Piglets

Dietary Moutan Cortex Radicis Improves Serum Antioxidant Capacity and Intestinal Immunity and Alters Colonic Microbiota in Weaned Piglets

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