Effects of Matricaria chamomilla Extract on Growth and Maturation of Isolated Mouse Ovarian Follicles in a Three-dimensional Culture System

Abstract

Background:The aim of this study was to design and assess the effects of hydroalcoholic extract of Matricaria chamomilla (MC) on preantral follicle culture of mouse ovaries in a three-dimensional culture system.Methods:Isolated preantral follicles were randomly divided into three main groups: the control group containing 10% fetal bovine serum without MC extract (G1), the first experimental group supplemented with 25 μg/ml hydroalcoholic extract of chamomile (G2), and the second experimental group supplemented with 50 μg/ml hydroalcoholic extract of chamomile (G3).Results:After 12 days of culture, the survival rate (P < 0.05), antrum formation (P < 0.01), metaphase two oocytes (P < 0.01), and the expression of PCNA (P < 0.05) and FSHR (P < 0.05) genes significantly decreased in G3 as compared with G1. On the other hand, at the last day of culture (day 12), the mean diameter of follicles cultured in the medium which was supplemented with 50 μg/ml hydroalcoholic extract of chamomile significantly decreased as compared with the G1 (P < 0.05). In addition, the levels of progesterone and dehydroepiandrosterone hormones significantly increased in the medium of G3 relative to G1 (P < 0.01), while in the medium of G1, the level of 17β-estradiol was significantly higher than that of other groups (P < 0.01). Reactive oxygen species levels of metaphase II oocytes were significantly decreased in G2 as compared with G1 (P < 0.01).Conclusion:Adding chamomile extract to culture media appeared to decrease follicular function and development.

The ameliorative effect of Galega officinalis Extract on Histological Damages, Oxidative Stress Induced by Torsion-Detorsion in Adult Rats’ Ovarian

“…The reproductive life of women or even thereafter can be affected by oxidative stress (5,6,15,16). Oxidative stress has been considered as an inconstancy between the generation of ROS and antioxidant defense capability of the body 122 (4)(5)(6)(7)9,14,15,17). The function of ROS has a similarity to the double-edged sword; one of their activities involves in body physiological processes as key signal molecules, but ROS have the main role in pathological damage in the reproductive system (15,16,18).…”

“…The function of ROS has a similarity to the double-edged sword; one of their activities involves in body physiological processes as key signal molecules, but ROS have the main role in pathological damage in the reproductive system (15,16,18). For instance, in many physiological processes such as oocyte maturation, fertilization, development of embryo, as well as pregnancy, ROS are effective (15)(16)(17).…”

Protective effects of the hydroalcoholic extract of Fumaria parviflora on testicular injury induced by torsion/detorsion in adult rats

“…On the other hand, IR injury involves alteration to microvascular blood flow, generation of reactive oxygen species (ROS), lipid peroxidation, neutrophil recruitment, pro‐inflammatory cytokines, apoptosis and the release of massive intracellular Ca 2+ that can lead to infertility (Karaguzel et al., ). ROS consist of radical oxygen species, such as hydroxyl radical (), superoxide anion () and nonradical oxygen species, such as hydrogen peroxide (H 2 O 2 ) and singlet oxygen ( 1 O 2 ) (Shoorei et al., ; Volpe, Villar‐Delfino, Anjos, & Nogueira‐Machado, ). The increased ROS generation leads to oxidative stress, activates apoptosis, as well as increased DNA damage (Meštrović et al., ; Roshangar, Rad, & Afsordeh, ; Roushangar & Rad, ; Volpe et al., ).…”

“…Also, some protocols are intended for the inhibition of oxidative stress; for example, using zinc aspartate, curcumin and dexamethasone is useful to reduce IR injury and increase the activity of antioxidant enzyme systems (Mogilner et al., ; Özkan, Boran, Kilinc, Garipardic, & Kurutaş, ). It has been claimed that plant‐derived antioxidants, such as flavonoids and polyphenols, are cost‐effective and accessible, have fewer complications and biological impacts than chemical drugs, can be protective therapeutic agents against ROS and therefore may be useful for male or female infertility (Alizadeh et al., ; Nasrabadi, Aboutalebi, & Naseri, ; Shoorei et al., ). Fumaria parviflora Lam.…”

The effect of clomiphene citrate and human chorionic gonadotropin on the expression of CatSper1, CatSper2, LHCGR, and SF1 genes, as well as the structural changes in testicular tissue of adult rats

“…Placental HCG is an alternative molecule for pituitary LH to control the synthesis of progesterone at the beginning of pregnancy from the implantation of the embryo to the sixth week of gestation. The syncytiotrophoblast cells can produce progesterone, independent of the stimulation by HCG, from the sixth week of pregnancy until delivery (Ascoli, Fanelli, & Segaloff, 2002;Cole, 1997;Keay, Vatish, Karteris, Hillhouse, & Randeva, 2004;Shoorei et al, 2018). However, there are many conflicting studies about the role of HCG.…”