Effects of medium chain triglycerides on hepatic fatty acid oxidation in clofibrate-fed newborn piglets

Zhao, Jinan; Pike, Brandon; Huang, Jin; Zhang, Feng; Odle, Jack; Lin, Xi

doi:10.1016/j.aninu.2022.12.001

Cited by 4 publications

(3 citation statements)

References 36 publications

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“…An interaction was detected between clofibrate and dietary treatment on palmitic acid oxidation in intestinal mucosa in this study. Administration of clofibrate increased the 14 C accumulation in CO 2 , ASP and total (CO 2 + ASP) in pigs receiving diets containing Succ, TC5 and TMPA, and the increases were consistent with the results previously observed in liver and kidney of neonatal pigs receiving dietary clofibrate [ 19 , 21 , 56 , 57 , 58 ] and by oral gavage [ 19 , 21 ]. This result suggests that the response of fatty acid oxidation in intestinal mucosa to PPARα activation is similar to other tissues in neonatal pigs.…”

Section: Discussionsupporting

confidence: 89%

“…Fatty acid oxidation was measured using the whole fresh homogenate from the intestinal mucosa processing as previously described [ 57 ]. Assays were performed using [1- 14 C]-palmitic acid (0.5 mM, 0.28 kBq/µmol) as a substrate with and without L659699 (1.6 µM), an inhibitor of HMGCS, iodoacetamide (50 µM), an inhibitor of AACD and carnitine (1 mM).…”

Section: Methodsmentioning

confidence: 99%

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Intestinal Carnitine Status and Fatty Acid Oxidation in Response to Clofibrate and Medium-Chain Triglyceride Supplementation in Newborn Pigs

Pike

Zhao

Hicks

et al. 2023

IJMS

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To investigate the role of peroxisome proliferator-activated receptor alpha (PPARα) in carnitine status and intestinal fatty acid oxidation in neonates, a total of 72 suckled newborn piglets were assigned into 8 dietary treatments following a 2 (±0.35% clofibrate) × 4 (diets with: succinate+glycerol (Succ), tri-valerate (TC5), tri-hexanoate (TC6), or tri-2-methylpentanoate (TMPA)) factorial design. All pigs received experimental milk diets with isocaloric energy for 5 days. Carnitine statuses were evaluated, and fatty acid oxidation was measured in vitro using [1-14C]-palmitic acid (1 mM) as a substrate in absence or presence of L659699 (1.6 µM), iodoacetamide (50 µM), and carnitine (1 mM). Clofibrate increased concentrations of free (41%) and/or acyl-carnitine (44% and 15%) in liver and plasma but had no effects in the intestine. The effects on carnitine status were associated with the expression of genes involved in carnitine biosynthesis, absorption, and transportation. TC5 and TMPA stimulated the increased fatty acid oxidation rate induced by clofibrate, while TC6 had no effect on the increased fatty acid oxidation induced by clofibrate (p > 0.05). These results suggest that dietary clofibrate improved carnitine status and increased fatty acid oxidation. Propionyl-CoA, generated from TC5 and TMPA, could stimulate the increased fatty acid oxidation rate induced by clofibrate as anaplerotic carbon sources.

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Section: Discussionsupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Intestinal Carnitine Status and Fatty Acid Oxidation in Response to Clofibrate and Medium-Chain Triglyceride Supplementation in Newborn Pigs

Pike

Zhao

Hicks

et al. 2023

IJMS

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“…C20:5 is a ligand for peroxisome proliferator activator receptor (PPAR) ( Grygiel-Górniak, 2014 ). Milk is a rich source of lipids, and growing evidence supports PPAR mediated metabolic pathways must be initiated shortly after birth so neonates can catabolize fats ( Hashimoto and Ogawa, 2018 ; Zhao et al, 2023 ; Shim et al, 2018 ; Yu et al, 1998 ). Expression of PPARα and PPARAγ was found significantly higher in peripheral blood mononuclear cells of breastfed versus formula-fed infants ( Cheshmeh et al, 2020 , 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Low colostrum intake results in potential accumulation of peroxisome lipid substrates in vaginal tissue of 3-week-old gilts

et al. 2023

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Low colostrum intake relates to poorer health and infertility in swine. We previously connected vaginal lipid profiles at weaning to fertility of sows. We hypothesized vaginal lipidome varied with colostrum intake. Our objective was to determine whether indicators of colostrum intake, immunocrit (IM) and weight gain 24 h postnatal (PN), related to vaginal lipids at d21 PN. Gilts (n=60) were weighed and blood sampled to measure IM. On d21 PN vaginal swabs were taken and lipids measured using multiple reaction monitoring. Abundance of multiple lipids differed (P<0.05) between gilts categorized as high versus low IM and high versus low 24 h gain. The abundance of multiple lipids correlated with IM and 24 h gain. Phosphatidylcholine PC(36:3), PC(36:2), and arachidonic acid (C20:4) positively (P<0.05) correlated with IM. The ether lipid PCo(38:6) and multiple cholesteryl esters negatively (P<0.05) correlated with IM. ROC analysis indicated arachidonic acid and docosanoic acid (C22:0) may serve as excellent biomarkers that distinguish between high and low IM. Similar to gilts found to be infertile, lipid profiles of low colostrum intake animals had greater abundance of very long chain fatty acids, lipids with high levels of unsaturation, and cholesteryl esters, which are metabolized in peroxisomes indicating their potential dysfunction.

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Intermittent fasting associated with coconut oil (Cocos nucifera L.) alters gut-liver axis parameters in diet-induced obese rats

de Souza,

Cavalcante,

dos Santos Lima

et al. 2024

Nutrition

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Effects of medium chain triglycerides on hepatic fatty acid oxidation in clofibrate-fed newborn piglets

Cited by 4 publications

References 36 publications

Intestinal Carnitine Status and Fatty Acid Oxidation in Response to Clofibrate and Medium-Chain Triglyceride Supplementation in Newborn Pigs

Intestinal Carnitine Status and Fatty Acid Oxidation in Response to Clofibrate and Medium-Chain Triglyceride Supplementation in Newborn Pigs

Low colostrum intake results in potential accumulation of peroxisome lipid substrates in vaginal tissue of 3-week-old gilts

Intermittent fasting associated with coconut oil (Cocos nucifera L.) alters gut-liver axis parameters in diet-induced obese rats

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