Effects of methanolic extract form Fuzhuan brick-tea on hydrogen peroxide-induced oxidative stress in human intestinal epithelial adenocarcinoma Caco-2 cells

Song, Jia‐Le; Gao, Yang

doi:10.3892/mmr.2014.1884

Cited by 19 publications

(13 citation statements)

References 58 publications

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“…Pretreatment of FBTA and gallic acid lessened the photo-toxicity triggered by UVB exposure, thereby protecting keratinocyte cells against UVB irradiation. Various concentrations of FBT (50–200 μg/mL) did not exhibit cytotoxicity towards Caco-2 cells and also protected the cells against H 2 O 2 -induced oxidative stress [30]. Our results are supported by previous findings that, FBTA had dermato-protective properties against UVB irradiation.…”

Section: Discussionsupporting

confidence: 89%

Protection of UVB-Induced Photoaging by Fuzhuan-Brick Tea Aqueous Extract via MAPKs/Nrf2-Mediated Down-Regulation of MMP-1

2018

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Ultraviolet B (UVB) irradiation is viewed as the principal inducer of skin photo-aging, associated with acceleration of collagen degradation and upregulation of matrix metalloproteinases (MMPs). The ethnic groups of southern/western China use Fuzhuan brick-tea (FBT) as a beverage and as a nutritional supplement. In this study, we scrutinized the antagonistic effects of aqueous extract of Fuzhuan-brick tea (FBTA) on skin photo-aging in UVB-exposed human keratinocyte (HaCaT) cells. FBTA exhibited strong antioxidant activity and quenched UVB-induced generation of cellular reactive oxygen species (ROS) without showing any toxicity. FBTA was capable of combating oxidative stress by augmenting messenger RNA (mRNA) and protein levels of both phase I and phase II detoxifying enzymes, especially heme oxygenase 1 (HO-1), by upregulating the nuclear factor erythroid 2-related factor 2 (Nrf2)-mediated pathway in HaCaT cells via the phosphorylation of p38 and extracellular signal-regulated kinase (ERK). FBTA also downregulated the expression of matrix metalloproteinase-1 (MMP-1) while upregulating type I procollagen by modulating Nrf2 signaling in UVB-irradiated HaCaT cells. Collectively, our results show that FBTA might be useful as a functional food while being a good candidate in the development of cosmetic products and medicines for the remedy of UVB-induced skin photo-aging.

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Section: Discussionsupporting

confidence: 89%

Protection of UVB-Induced Photoaging by Fuzhuan-Brick Tea Aqueous Extract via MAPKs/Nrf2-Mediated Down-Regulation of MMP-1

2018

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“…Hydrogen peroxide (H2O2) above 150 μM significantly decreased cell viability, and 1000 μM H2O2 further reduced cell viability to about 50% (Figure 2A). Similar results that 1 mM H2O2 treated for 6 h significantly reduced the cell viability of Caco-2 were observed [15]. Additionally, treatments with 0.8 mM H2O2 for 24 h led to increased release of lactate dehydrogenase (LDH), an indicator of cell membrane injury in IPEC-J2 cells [16].…”

Section: Hydrogen-peroxide-induced Oxidative Stress In Caco-2 Cellssupporting

confidence: 76%

Targeted Isolation of Antioxidant Constituents from Plantago asiatica L. and In Vitro Activity Assay

et al. 2020

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Plantago asiatica L. is widely distributed in Eastern Asia and a commonly used drug in China, Korea, and Japan for diuretic and antiphlogistic purposes. In this experiment, the present study was performed to isolate antioxidant molecules based on the DPPH scavenging activity assay and discover the bioactive compounds which contributed to performing the function of Plantago asiatica L. Each faction was chosen for further isolation guided by DPPH scavenging activity assay. Afterwards, two potential bioactive molecules, aesculetin and apigenin, were isolated for in vitro antioxidant activity in cells. Hydrogen-peroxide-induced oxidative stress led to decreased cell viability, impaired intercellular junction, and damage to the cell membrane and DNA. Furthermore, aesculetin ameliorated decreased cell viability induced by hydrogen peroxide via upregulation of antioxidant related genes, and apigenin also protected against H2O2 mainly by improving the glutathione (GSH) antioxidant system, such as increasing the activity of glutathione peroxidase (GPX), glutathione reductase (GR), and the ration of GSH/glutathione disulfide (GSSG). Above all, these findings suggest that aesculetin and apigenin may be bioactive compounds for antioxidant function in Plantago asiatica L.

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“…Isopropanol was added to the supernatant at a 1: 1 ratio and the RNA was piloted by centrifugation (12, [25]. β-actin was used for normalization.…”

Section: Reverse Transcription Polymerase Chain Reaction (Rt-pcr) Assaymentioning

confidence: 99%

Study on soybean milk fermented by Lactobacillus plantarum YS-1 reduced the H2O2-induced oxidative damage in Caco-2 cells

Li¹,

Long²,

Pan³

et al. 2018

biomedicalresearch

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This study was conducted to investigate the free radicals scavenging activity and cytoprotective effects of Lactobacillus plantarum YS-1 Fermented Soy Milk (FSM-LPYS1) in Caco-2 human colon adenocarcinoma cells damaged by hydrogen peroxide (H 2 O 2 )-induced oxidation. FSM-LPYS1 showed a remarkable in vitro activity against 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) and hydroxyl radical (•OH). FSM-LPYS1 significantly decreased the levels of intracellular Reactive Oxygen Species (ROS), lipid peroxidation, and enhanced the activities and expression levels of Catalase (CAT), Superoxide Dismutase (SOD) and glutathione peroxidase (GSH-Px) in H 2 O 2 -treated Caco-2 cells (p<0.05). FSM-LPYS1 also reduced the protein levels of IL-8 and NF-κB in Caco-2 cells. These results clearly indicated that FSM-LPYS1 had impressively scavenged free radicals and were able to reduce the H 2 O 2 -induced oxidative damage in Caco-2 cells by reducing the intracellular ROS generation, restraining lipid peroxidation, and by raising the endogenous antioxidant enzyme activity. In addition, the FSM-LPYS1 could also reduce the H 2 O 2 -induced inflammation reaction. FSM-LPYS1 is a functional food which has antioxidant properties and it can be used as high quality soybean processing food for exploitation of health benefits.

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Effects of methanolic extract form Fuzhuan brick-tea on hydrogen peroxide-induced oxidative stress in human intestinal epithelial adenocarcinoma Caco-2 cells

Cited by 19 publications

References 58 publications

Protection of UVB-Induced Photoaging by Fuzhuan-Brick Tea Aqueous Extract via MAPKs/Nrf2-Mediated Down-Regulation of MMP-1

Protection of UVB-Induced Photoaging by Fuzhuan-Brick Tea Aqueous Extract via MAPKs/Nrf2-Mediated Down-Regulation of MMP-1

Targeted Isolation of Antioxidant Constituents from Plantago asiatica L. and In Vitro Activity Assay

Study on soybean milk fermented by Lactobacillus plantarum YS-1 reduced the H2O2-induced oxidative damage in Caco-2 cells

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