Effects of Natural Eggshell Membrane (NEM) on Cytokine Production in Cultures of Peripheral Blood Mononuclear Cells: Increased Suppression of Tumor Necrosis Factor-<i>α</i> Levels After <i>In Vitro</i> Digestion

Benson, Kathleen F.; Ruff, Kevin J.; Jensen, Gitte S.

doi:10.1089/jmf.2011.0197

Cited by 38 publications

(33 citation statements)

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“…Of these subjects, twenty-seven (61%) were female and seventeen (39%) were male. The treated joints consisted of knee (39), hip (14), ankle (3), both either knee and hip (10), or both knee and ankle (2). Of the thirty-nine subjects with knee OA, sixteen (40.0%) had bilateral incidence.…”

Section: Resultsmentioning

confidence: 99%

“…A number of these constituents have been shown previously to be beneficial in the treatment of OA [12,13]. Eggshell membrane itself has been shown both in vitro [14] and in vivo [15] to reduce various pro-inflammatory cytokines, including interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-a), two primary mediators of inflammation. A U.S. company, ESM Technologies, LLC (Carthage, MO USA), has developed methods to efficiently and effectively separate eggshell membrane from eggshells on a commercial metric-ton scale.…”

Section: Introductionmentioning

confidence: 99%

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NEM Brand Eggshell Membrane Effective in the Treatment of Pain Associated with Knee and Hip Osteoarthritis: Results from a Six Center, Open Label German Clinical Study

Danesch¹

2014

J Arthritis

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Objective: NEM ® brand eggshell membrane is a novel dietary supplement ingredient that contains naturally occurring glycosaminoglycans and proteins essential for maintaining healthy joints. A six center, open label clinical study was conducted to evaluate the efficacy and safety of NEM ® as a treatment for pain and inflexibility associated with osteoarthritis of the knee and/or hip in a European population.Methods: Forty-four subjects received oral NEM ® 500 mg once daily for eight weeks. The primary outcome measure was to evaluate the mean effectiveness of NEM ® in relieving general pain associated with moderate osteoarthritis of the knee and/or hip at 10,30 and 60 days utilizing a 10-question abbreviated questionnaire based on the WOMAC osteoarthritis questionnaire. Results: Supplementation with NEM® produced a significant treatment response from baseline at 10 days (Q1-6 and Q9) (8.6% to 18.1% improvement) and at 30 and 60 days for all nine pain-related questions evaluated (22.4% to 35.6% improvement) and at 30 and 60 days for stiffness (Q10)(27.4% to 29.3% improvement). In a Patient's Global Assessment, greater than 59% of patients rated the efficacy of NEM ® as good or very good following 60 days of supplementation. Physicians also rated the treatment effective in subjects, with greater than 75% having moderate or significant improvement from baseline after 60 days. There were no serious adverse events reported during the study and the treatment was reported to be well tolerated. Conclusions: Supplementation with NEM® significantly reduced pain, both rapidly (10 days) and continuously (60 days) demonstrating that it is a safe and effective therapeutic option for the treatment of pain associated with osteoarthritis of the knee and/or hip. Results from previous clinical studies on NEM ® can likely be extended to the broader European population.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

NEM Brand Eggshell Membrane Effective in the Treatment of Pain Associated with Knee and Hip Osteoarthritis: Results from a Six Center, Open Label German Clinical Study

Danesch¹

2014

J Arthritis

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“…Hen egg white glycoprotein, ovotransferrin, also present in ESM, has recently been demonstrated to possess anti-inflammatory properties 15. Some studies have indicated anti-inflammatory properties of hydrolyzed and solubilized ESM 16–19. It is unknown whether processed ESM powder possesses the same properties.…”

Section: Introductionmentioning

confidence: 99%

The extracellular matrix of eggshell displays anti-inflammatory activities through NF-κB in LPS-triggered human immune cells

Vuong

Rønning

Suso³

et al. 2017

JIR

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Avian eggshell membrane (ESM) is a natural biomaterial that has been used as an alternative natural bandage on burned and cut skin injuries for >400 years in Asian countries, and is available in large quantities from egg industries. Our aim was to characterize ESM that was separated and processed from egg waste, and to study whether this material possesses anti-inflammatory properties, making it suitable as an ingredient in industrial production of low cost wound healing products. Our results show that the processed ESM particles retain a fibrous structure similar to that observed for the native membrane, and contain collagen, and carbohydrate components such as hyaluronic acid and sulfated glycosaminoglycans, as well as N-glycans, mostly with uncharged structures. Furthermore, both processed ESM powder and the ESM-derived carbohydrate fraction had immunomodulation properties in monocytes and macrophage-like cells. Under inflammatory conditions induced by lipopolysaccharide, the ESM powder and the isolated carbohydrate fraction reduced the activity of the transcription factor nuclear factor-κB. The expression of the immune regulating receptors toll-like receptor 4 and ICAM-1, as well as the cell surface glycoprotein CD44, all important during inflammation response, were down-regulated by these fractions. Interestingly, our experiments show that the two fractions regulated cytokine secretion differently: ESM depressed inflammation by increased secretion of the anti-inflammatory cytokine IL-10 while the carbohydrate fraction reduced secretions of the pro inflammatory cytokines IL-1β and IL-6. Also, the phosphorylation of p65 and p50 subunits of nuclear factor-κB, as well as nuclear localization, differed between processed ESM powder and carbohydrate fraction, suggesting different down-stream regulation during inflammation. In conclusion, processed ESM powder and its soluble carbohydrate components possess anti-inflammatory properties, demonstrating the potential of ESM as a novel biological wound dressing for treatment of chronic inflammatory wounds.

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“…In addition, EMS has been also used in medical industries as a possible new effective and safe therapeutic option for the treatment of pain and inflexibility associated with joint and connective tissue (JCT) disorders (Ruff et al 2009). ESM has found applications in ophthalmology as a cheap and versatile eye model which could be used during vitreous surgery training (Benson et al 2012). Moreover; ESM was found to represent an interesting biomaterial for electrochemical application such as a separator in supercapacitors (Li et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Synthesis of nanostructured pirochromite magnesium chromate with egg shell membrane template

Ramachandran

Hamed

2016

Appl Nanosci

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In this work, we report on the use of fresh and boiled egg shell membrane as bio-templates in the coprecipitation of pirochromite. The structure evolution, microstructure and optical properties of the ESM templated MgCr 2 O 4 nanomaterial were studied by X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, Energy dispersive X-ray spectroscopy, elemental mapping and UV-Vis-NIR spectrophotometry. The annealing of the untemplated and templated co-precipitated MgCr 2 O 4 powders at 1000°C for 4 h produced single phased nanostructured material with spinel cubic crystal structure. The FTIR results showed slight variations in the band positions m 1 and m 2 which are attributed to the change in the microstructure due to the introduction of ESM in the preparation of MgCr 2 O 4 . The morphologies and average crystallite size of the annealed MgCr 2 O 4 nanocrystalline powders depended on the type of the template. 3D hierarchical flake-like and mesh like structures were observed. The annealed MgCr 2 O 4 nanocrystalline powders have shown excitonic absorptions in the visible range 300-500 nm due to the transitions that took place from the O-2p level to the Cr-3d level. The optical band gap energies were found to be 3.68-3.71 eV for the direct band gap and 3.30-3.37 eV for the indirect band gap. This could make these MgCr 2 O 4 nanocrystalline powders possible photocatalysts in the visible range 300-500 nm.

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Effects of Natural Eggshell Membrane (NEM) on Cytokine Production in Cultures of Peripheral Blood Mononuclear Cells: Increased Suppression of Tumor Necrosis Factor-α Levels After In Vitro Digestion

Cited by 38 publications

References 20 publications

NEM Brand Eggshell Membrane Effective in the Treatment of Pain Associated with Knee and Hip Osteoarthritis: Results from a Six Center, Open Label German Clinical Study

NEM Brand Eggshell Membrane Effective in the Treatment of Pain Associated with Knee and Hip Osteoarthritis: Results from a Six Center, Open Label German Clinical Study

The extracellular matrix of eggshell displays anti-inflammatory activities through NF-κB in LPS-triggered human immune cells

Synthesis of nanostructured pirochromite magnesium chromate with egg shell membrane template

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Effects of Natural Eggshell Membrane (NEM) on Cytokine Production in Cultures of Peripheral Blood Mononuclear Cells: Increased Suppression of Tumor Necrosis Factor-α Levels After In Vitro Digestion

Cited by 38 publications

References 20 publications

NEM Brand Eggshell Membrane Effective in the Treatment of Pain Associated with Knee and Hip Osteoarthritis: Results from a Six Center, Open Label German Clinical Study

NEM Brand Eggshell Membrane Effective in the Treatment of Pain Associated with Knee and Hip Osteoarthritis: Results from a Six Center, Open Label German Clinical Study

The extracellular matrix of eggshell displays anti-inflammatory activities through NF-&kappa;B in LPS-triggered human immune cells

Synthesis of nanostructured pirochromite magnesium chromate with egg shell membrane template

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Product

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The extracellular matrix of eggshell displays anti-inflammatory activities through NF-κB in LPS-triggered human immune cells