Effects of Neurolytic Celiac Plexus Block on Liver Regeneration in Rats with Partial Hepatectomy

Abstract

Liver regeneration is the basic physiological process after partial hepatectomy (PH), and is important for the functional rehabilitation of the liver after acute hepatic injury. This study was designed to explore the effects of neurolytic celiac plexus block (NCPB) on liver regeneration after PH. We established a model of PH in rats, assessing hepatic blood flow, liver function, and serum CRP, TNF-α, IL-1β and IL-6 concentrations of the residuary liver after PH. Additionally, histopathological studies, immunohistochemistry, and western blotting were also performed. Our results indicated that NCPB treatment after PH improved liver regeneration and survival rates, increased hepatic blood flow, reduced hepatocyte damage, decreased the secretion and release of inflammatory cytokines, increased the expression of B cell lymphoma/leukemia-2 (Bcl-2), and decreased the expression of Bcl-2 associated X protein (Bax). Additionally, Western blotting revealed that the expression of NF-κB p65 and c-Jun were decreased in liver after NCPB. In conclusion, the results of our present study indicate that NCPB treatment has a favorable effect on liver regeneration after PH. We suggest that NCPB can be utilized as an effective therapeutic method to help the functional rehabilitation of the liver after acute hepatic injury or liver cancer surgery.

Hepatoprotective effect of Fufang-Huanglu oral liquid on α- naphthylisothiocyanate-induced hepatitis jaundice in mice

“…The current study successfully established the model by using ANIT, and demonstrated that HOL could decrease liver index and degree of liver injury in the experimental model mice, indicating that HOL possesses potential hepatoprotective effects. Elevated SGPT and SGOT are two characteristic markers for hepatic injury [2,13,14]. In this study, the levels of SGPT and SGOT were sharply increased after ANIT treatment.…”

“…It is well known that the liver is a vital intraabdominal organ which performs important biological functions such as bile secretion, synthesis of plasma proteins and lipid biosynthesis [1,2]. It is known that liver injury may arise from excessive alcohol intake, as well as exposure to various toxins and environmental pollutants.…”

Neurolytic celiac plexus block enhances skeletal muscle insulin signaling and attenuates insulin resistance in GK rats

“…Rats received 0.5% lidocaine (2 ml once/day) or saline under ether anesthesia. The puncture point was determined in accordance with a previous report (18). Briefly, rats were injected bilaterally at the first lumbar transverse process, the first bony protrusion under the root of the last rib.…”

“…Serum and quadriceps muscle samples for biochemical analyses were collected, which were stored at -80˚C until use. A 100 mg sample of freshly isolated soleus muscle was used to determine skeletal muscle 2-deoxy-D-glucose (2-DG) uptake in each rat (14). Soleus muscle bundles were placed in Krebs-Ringer bicarbonate buffer (OriGene Technologies, Inc., Beijing, China) with or without 1.0 µmol/ml insulin to measure insulin-stimulated and basal glucose uptake, respectively.…”

“…Our previous study of NCPB in rats following resection of 70% of the liver indicated that NCPB attenuated IR; this may be due to an inhibition of systemic inflammatory response syndrome that typically occurs following severe trauma (14). NCPB may therefore slow or reverse NIDDM-associated dysfunction via the suppression of immune-mediated IR.…”