Effects of Nitrate and L-Arginine on Content of Nitric Oxide and Activities of Antioxidant Enzymes in Roots of Wheat Seedlings and Their Heat Resistance

Karpets, Yu. V.; Kolupaev, Yu. Е.; Lugovaya, A. A.; Shvidenko, N. V.; Yastreb, Т. О.

doi:10.1134/s1021443718050096

Cited by 23 publications

(10 citation statements)

References 28 publications

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“…Besides phenolic compounds and the other compounds above mentioned, bearing in mind the data reported in literature about the antioxidant potential of amino acids and their derivatives, it is possible that the free amino acids detected in rosemary infusions also had an impact on their antioxidant properties. Notwithstanding, it is important to note that not all amino acids are endowed of antioxidant properties, and, besides the mechanisms of action previously mentioned in Section 3.3 , amino acids can also act by others—namely by enhancing the activity of antioxidant enzymes or by acting as oxidative stress biomarkers [ 15 , 43 ]. Nonetheless, considering the low concentration levels of amino acids (low ng/mL) compared to the high concentration levels of phenolic compounds (high ng/mL to low μg/mL), it is evident that phenolic compounds (including flavonoids and phenolic diterpenes) were the principal responsible for the antioxidant potential of rosemary infusions.…”

Section: Resultsmentioning

confidence: 99%

Comprehensive Phenolic and Free Amino Acid Analysis of Rosemary Infusions: Influence on the Antioxidant Potential

et al. 2021

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The phenolics profile, free amino acids composition, and antioxidant potential of rosemary infusions were studied. Forty-four compounds belonging to nine different groups (hydroxybenzoic acids, hydroxycinnamic acids, flavan-3-ols, flavanones, flavones, phenolic diterpenes, hydroxybenzaldehydes, coumarins, and pyranochromanones) were identified by UHPLC-ESI-Q-TOF-MS. Of these, seven were firstly described in rosemary infusions: a rosmanol derivative, two dihydroxycoumarin hexosides, a hydroxybenzaldehyde, a dihydroxybenzoic acid hexoside, coumaric acid hexoside, and isocalolongic acid. The free amino acid profile of the beverages was also reported by the first time with seven amino acids found (asparagine, threonine, alanine, tyrosine, phenylalanine, isoleucine, and proline). Furthermore, DPPH• scavenging ability, Ferric Reducing Antioxidant Power and Oxygen Radical Absorbance Capacity, as well as total phenolics and flavonoids contents, were assessed. Overall, rosemary infusions showed to be a very good source of antioxidants. A 200 mL cup of this infusion contributes to the ingestion of ~30 mg of phenolic compounds and about 0.5–1.1 μg of free amino acids. This type of beverages may present a positive impact on the maintenance of the body antioxidant status and contribute to the prevention of oxidative stress related diseases.

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Section: Resultsmentioning

confidence: 99%

Comprehensive Phenolic and Free Amino Acid Analysis of Rosemary Infusions: Influence on the Antioxidant Potential

et al. 2021

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“…Exogenous arginine treatment delays fruit coloring, inhibits fruit ripening, and increases the activity of antioxidant enzymes in strawberry fruits (Lv et al, 2020). Arginine also increases the N content in tomato (Wang et al, 2021) and NO content in wheat seedlings (Karpets et al, 2018). Overexpression of OsARG in rice (cv.…”

Section: Introductionmentioning

confidence: 99%

Arginine Increases Tolerance to Nitrogen Deficiency in Malus hupehensis via Alterations in Photosynthetic Capacity and Amino Acids Metabolism

et al. 2022

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Arginine plays an important role in the nitrogen (N) cycle because it has the highest ratio of N to carbon among amino acids. In recent years, there has been increased research interest in improving the N use of plants, reducing the use of N fertilizer, and enhancing the tolerance of plants to N deficiency. Here, the function of arginine in the growth of apple (Malus hupehensis) under N deficiency was explored. The application of 100 μmol L–1 arginine was effective for alleviating N-deficiency stress. Exogenous arginine promoted the absorption and use of N, phosphorus (P), and potassium (K) under low N stress. The net photosynthetic rate, maximal photochemical efficiency of photosystem II, and chlorophyll content were higher in treated plants than in control plants. Exogenous arginine affected the content of many metabolites, and the content of many amino acids with important functions was significantly increased, such as glutamate and ornithine, which play an important role in the urea cycle. Half of the metabolites were annotated to specialized metabolic pathways, including the synthesis of phenolic substances, flavonoids, and other substances with antioxidant activity. Our results indicate that arginine promotes the plant photosynthetic capacity and alters amino acid metabolism and some antioxidants including phenolic substances and flavonoids to improve the tolerance of apple to N deficiency, possibly through the improvement of arginine content, and the absorption of mineral.

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“…Besides the phytohormones, AAs have also various prominent functions in plant stress response. Arginine, as one of the most functional diverse AAs in living cells, has been reported to modify plant stress tolerance through the manipulation of antioxidant mechanisms (Karpets et al, 2018). However, the roles of arginine in alleviating HT stress-induced ROS damage in G. lemaneiformis are not yet known.…”

Section: Introductionmentioning

confidence: 99%

“…Arginine is a precursor for the biosynthesis of nitric oxide (NO), polyamines (PAs), which are related to stress resistance (Liu et al, 2006). Exogenous or pre-treated with arginine has been reported its positive effect via modification of those argininederived stress-related substances under abiotic stress, such as heat, salinity, water deficit, and heavy metal, respectively, in rice, wheat, sugarcane, and Hyoscyamus niger (Kakkar et al, 2000;Nasibi et al, 2013;Karpets et al, 2018;Silveira et al, 2021). The application of arginine can also play a vital role in enhancing the defense resistance system against diseases in tomato fruits via its effects on NO biosynthesis and defensive enzyme activity (Zheng et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…The application of arginine can also play a vital role in enhancing the defense resistance system against diseases in tomato fruits via its effects on NO biosynthesis and defensive enzyme activity (Zheng et al, 2011). In terms of temperature stress, exogenously applied arginine could alleviate the adverse effects of fluctuating temperature stress on maize growth and development (Matysiak et al, 2020) and enhance the seedling resistance to the damaging heating in collaboration with the NO-signaling system (Karpets et al, 2018). However, it remains unclear whether the unique roles of arginine in G. lemaneiformis are attributable to stressrelated substances under HT conditions.…”

Section: Introductionmentioning

confidence: 99%

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Physiological and Transcriptome Analysis of Exogenous L-Arginine in the Alleviation of High-Temperature Stress in Gracilariopsis lemaneiformis

Zhang

Liu

et al. 2021

Front. Mar. Sci.

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Gracilariopsis lemaneiformis (G. lemaneiformis) is an important marine red macroalgae with high economic and ecological value all over the world. To date, global warming is a key issue that has a great impact on all living organisms, such as macroalgae. L-arginine (Arg) is a precursor of nitric oxide (NO) and polyamines (PAs), which can induce stress defense responses in land plants. However, its role in inducing algae resistance at high temperature (HT) is unclear. In this study, G. lemaneiformis thalli were treated with different concentrations of Arg to investigate its effect and the mechanism on the tolerance of G. lemaneiformis against HT stress. It turned out that exogenous Arg significantly alleviated the HT-induced oxidative damage as indicated by a markedly decrease in malondialdehyde (MDA) content. Notably, Arg remarkably improved the relative growth rate (RGR) and phycobiliprotein (PBP) contents of G. lemaneiformis at HT. Moreover, Arg significantly elevated the activities of antioxidant enzymes, such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), to efficiently scavenge reactive oxygen species (ROS). In addition, it also promoted the accumulation of free amino acids (AAs) as compared to those in the control treatment (CK) group under HT conditions. To investigate the mechanism of G. lemaneiformis to Arg, a transcriptome analysis was performed and revealed 1,414 and 3,825 differentially expressed genes (DEGs) in Arg-treated groups as compared to CK groups at 24 and 48 h of HT stress, respectively. Results showed that Arg significantly upregulated the expression of genes encoding antioxidant enzymes, heat shock proteins, and triggered transcription factors (TFs) signaling during HT stress. Moreover, Arg enhanced the DEGs involved in arginine and proline (Pro) metabolism, AAs biosynthesis, glycolysis, tricarboxylic acid (TCA) cycle, and oxidative phosphorylation. These results may help in understanding the role of Arg in G. lemaneiformis resistance to HT and provide a practical viewpoint for obtaining heat-tolerant G. lemaneiformis to further promote the development of the cultivated seaweed industry in the future.

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Effects of Nitrate and L-Arginine on Content of Nitric Oxide and Activities of Antioxidant Enzymes in Roots of Wheat Seedlings and Their Heat Resistance

Cited by 23 publications

References 28 publications

Comprehensive Phenolic and Free Amino Acid Analysis of Rosemary Infusions: Influence on the Antioxidant Potential

Comprehensive Phenolic and Free Amino Acid Analysis of Rosemary Infusions: Influence on the Antioxidant Potential

Arginine Increases Tolerance to Nitrogen Deficiency in Malus hupehensis via Alterations in Photosynthetic Capacity and Amino Acids Metabolism

Physiological and Transcriptome Analysis of Exogenous L-Arginine in the Alleviation of High-Temperature Stress in Gracilariopsis lemaneiformis

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