Effects of nutrient supply methods and illumination with blue light emitting diodes (LEDs) on astaxanthin production by Haematococcus pluvialis

Lababpour, Abdolmajid; Hada, Keishi; Shimahara, Kazumichi; Katsuda, Tomohisa; Katoh, Shigeo

doi:10.1016/s1389-1723(05)00311-7

Cited by 44 publications

(13 citation statements)

References 7 publications

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“…For example, our results indicated that Haematococcus cells may react quickly and more sensitive to blue HL induction compared to white HL stress resulting in zeaxanthin and astaxanthin concentrations under the former than in the latter. This conclusion is similar to a previous study, in which red light emitting diodes (LEDs) operated at a relatively low light intensity were suitable for cell growth with replacement of medium, and LEDs emitting short wavelengths (380-470 nm) the morphological change of H. pluvialis and enhanced the accumulation of astaxanthin (Katsuda et al 2006;Lababpour et al 2004). Previous studies have demonstrated that the CHY involved in the astaxanthin biosynthetic pathway in H. pluvialis contains a cytochrome P450 by the use of ellipticine and that the main biosynthetic pathway is via canthaxanthin (Schoefs et al 2001).…”

Section: Discussionsupporting

confidence: 91%

Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis

Cui

Wang

et al. 2013

J Appl Phycol

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A full-length complementary DNA (cDNA) sequence of ε-ring CHY (designated Haecyp97c) was cloned from the green alga Haematococcus pluvialis by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends methods. The Haecyp97c cDNA sequence was 1,995 base pairs (bp) in length, which contained a 1,620-bp open reading frame, a 46-bp 5′-untranslated region (UTR), and a 329-bp 3′-UTR with the characteristic of the poly (A) tail. The deduced protein had a calculated molecular mass of 58.71 kDa with an estimated isoelectric point of 7.94. Multiple alignment analysis revealed that the deduced amino acid sequence of HaeCYP97C shared high identity of 72-85 % with corresponding CYP97Cs from other eukaryotes. The catalytic motifs of cytochrome P450s were detected in the amino acid sequence of HaeCYP97C. The transcriptional levels of Haecyp97c and xanthophylls accumulation under high light (HL) stress have been examined. The results revealed that Haecyp97c transcript was strongly increased after 13-28 h under HL stress. Meanwhile, the concentrations of chlorophylls, carotenes, and lutein were decreased, and zeaxanthin and astaxanthin concentrations were increased rapidly, respectively. These facts indicated that HaeCYP97C was perhaps involved in xanthophyll biosynthesis, which plays an important role in adaption to HL for H. pluvialis.

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Section: Discussionsupporting

confidence: 91%

Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis

Cui

Wang

et al. 2013

J Appl Phycol

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“…Therefore, we propose that Haematococcus cells are more sensitive to blue induction compare with white. Similar phenomena were reported red Light Emitting Diodes (LEDs) operated at a relatively low light intensity were found to be suitable for cell growth; and LEDs emitting short wavelength (380–470 nm) can induce morphological changes in H. pluvialis and enhance astaxanthin accumulation [92-94]. It is well known that strong light (blue) enhances astaxanthin accumulation, because astaxanthin is produced by H. pluvialis cells to protect cells from the strong intensity of the light [93].…”

Section: Resultsmentioning

confidence: 63%

Evolutionary origins, molecular cloning and expression of carotenoid hydroxylases in eukaryotic photosynthetic algae

Cui

Wang

et al. 2013

BMC Genomics

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BackgroundXanthophylls, oxygenated derivatives of carotenes, play critical roles in photosynthetic apparatus of cyanobacteria, algae, and higher plants. Although the xanthophylls biosynthetic pathway of algae is largely unknown, it is of particular interest because they have a very complicated evolutionary history. Carotenoid hydroxylase (CHY) is an important protein that plays essential roles in xanthophylls biosynthesis. With the availability of 18 sequenced algal genomes, we performed a comprehensive comparative analysis of chy genes and explored their distribution, structure, evolution, origins, and expression.ResultsOverall 60 putative chy genes were identified and classified into two major subfamilies (bch and cyp97) according to their domain structures. Genes in the bch subfamily were found in 10 green algae and 1 red alga, but absent in other algae. In the phylogenetic tree, bch genes of green algae and higher plants share a common ancestor and are of non-cyanobacterial origin, whereas that of red algae is of cyanobacteria. The homologs of cyp97a/c genes were widespread only in green algae, while cyp97b paralogs were seen in most of algae. Phylogenetic analysis on cyp97 genes supported the hypothesis that cyp97b is an ancient gene originated before the formation of extant algal groups. The cyp97a gene is more closely related to cyp97c in evolution than to cyp97b. The two cyp97 genes were isolated from the green alga Haematococcus pluvialis, and transcriptional expression profiles of chy genes were observed under high light stress of different wavelength.ConclusionsGreen algae received a β-xanthophylls biosynthetic pathway from host organisms. Although red algae inherited the pathway from cyanobacteria during primary endosymbiosis, it remains unclear in Chromalveolates. The α-xanthophylls biosynthetic pathway is a common feature in green algae and higher plants. The origination of cyp97a/c is most likely due to gene duplication before divergence of green algae and higher plants. Protein domain structures and expression analyses in green alga H. pluvialis indicate that various chy genes are in different manners response to light. The knowledge of evolution of chy genes in photosynthetic eukaryotes provided information of gene cloning and functional investigation of chy genes in algae in the future.

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“…Numerous studies have concluded to investigate the cultivation of H. pluvialis on new techniques and methods such as one-step production, attached cultivation, immobilized biofilm cultivation and kinetic models for astaxanthin production, while the industrial application was not desirable 36 37 38 39 . Created bioreactors, new operating system and the application of computer technique have a certain requirement for equipment and operators that restricts their application timely and widely.…”

Section: Discussionmentioning

confidence: 99%

Repeated cultivation: non-cell disruption extraction of astaxanthin for Haematococcus pluvialis

Sun

Guan

Kong

et al. 2016

Sci Rep

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The operation of cell disruption is indispensable but cost much in microalgae industry. To be simplified, two different reaction mechanisms await in the cell to respond to moderated or stressed environment. The physical and chemical changes of enzyme and turgor pressure of cell in this conversion play an important role in the enhancement of biomass and metabolites. Repeated turgor pressure (based on the structure and mechanics of cell wall) and converted enzyme system (based on photosynthesis) were used to loosen cell wall and then repeated cultivation of Haematococcus pluvialis for astaxanthin extraction was proposed. There was no significant difference of extraction yield between the broken cell (94.75 ± 3.13%) and non-broken cell (92.32 ± 3.24%) treated by the repeated cultivation. Meanwhile, fed-batch culture according to the relationship among pH and nutrient concentration was used to enhance the biomass of Haematococcus pluvialis with the dry cell weight of 1.63 ± 0.07 g/L.

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Effects of nutrient supply methods and illumination with blue light emitting diodes (LEDs) on astaxanthin production by Haematococcus pluvialis

Cited by 44 publications

References 7 publications

Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis

Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis

Evolutionary origins, molecular cloning and expression of carotenoid hydroxylases in eukaryotic photosynthetic algae

Repeated cultivation: non-cell disruption extraction of astaxanthin for Haematococcus pluvialis

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