Effects of Ovariectomy on Rat Mandibular Cortical Bone: A Study Using Raman Spectroscopy and Multivariate Analysis

Fu, Xiaoming; Chen, Jiang; Wu, Dong; Du, Zhibin; Lei, Qun; Cai, Zhiyu; Schultze‐Mosgau, Stefan

doi:10.1021/ac300046x

Cited by 12 publications

(17 citation statements)

References 40 publications

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“…Fu et al 49 demonstrated how the linear discriminants of PCA separate compositional changes in the rat mandible after ovariectomy. PCA has also been used to chart developmental changes in differentiating mesenchymal stem cells 50 as well as RS signatures of cartilaginous zones during fetal femur development.…”

Section: Discussionmentioning

confidence: 99%

Polarization in Raman spectroscopy helps explain bone brittleness in genetic mouse models

et al. 2014

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Abstract. Raman spectroscopy (RS) has been extensively used to characterize bone composition. However, the link between bone biomechanics and RS measures is not well established. Here, we leveraged the sensitivity of RS polarization to organization, thereby assessing whether RS can explain differences in bone toughness in genetic mouse models for which traditional RS peak ratios are not informative. In the selected mutant miceactivating transcription factor 4 (ATF4) or matrix metalloproteinase 9 (MMP9) knock-outs-toughness is reduced but differences in bone strength do not exist between knock-out and corresponding wild-type controls. To incorporate differences in the RS of bone occurring at peak shoulders, a multivariate approach was used. Full spectrum principal components analysis of two paired, orthogonal bone orientations (relative to laser polarization) improved genotype classification and correlation to bone toughness when compared to traditional peak ratios. When applied to femurs from wild-type mice at 8 and 20 weeks of age, the principal components of orthogonal bone orientations improved age classification but not the explanation of the maturation-related increase in strength. Overall, increasing polarization information by collecting spectra from two bone orientations improves the ability of multivariate RS to explain variance in bone toughness, likely due to polarization sensitivity to organizational changes in both mineral and collagen.

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Section: Discussionmentioning

confidence: 99%

Polarization in Raman spectroscopy helps explain bone brittleness in genetic mouse models

et al. 2014

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“…It was found that higher viscoelastic energy dissipation occurs in tissue regions with a lower mineral-to-matrix ratio (Pathak et al, 2011). The mineral-to-matrix ratios of rat mandibles and lumbar vertebrae were measured to be 20 and 7.9, respectively (Fu et al, 2012; Otomo et al, 2004). In the current study, the slopes of viscosity-to-modulus curves for rat mandibles and lumbar vertebrae were found to be 1221.307 and 557.625, respectively (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…It was indicated that the mineral-to-matrix ratio was lower in newly-formed bone tissue regions than in pre-existing old bone tissue regions when mouse femurs were observed under Raman spectroscopy (Pathak et al, 2011). The mineral-to-matrix ratio of bone tissue also varies depending on anatomical site (Fu et al, 2012; Otomo et al, 2004). Pathak et al (2011) found a larger viscoelastic response in a region of tissue with a smaller mineral-to-matrix ratio using nanoindentation-based dynamic mechanical analysis.…”

Section: Introductionmentioning

confidence: 99%

Effect of estrogen deficiency on regional variation of a viscoelastic tissue property of bone

Huja

Navalgund

et al. 2013

Journal of Biomechanics

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Estrogen deficiency changes the regional distribution of tissue mineral density leading to alteration of the mechanical properties of bone at the tissue level. Direct measurement of the regional variation of elastic modulus and viscosity, which is the capacity to resist time-dependent viscoelastic deformation, will aid in our understanding of how estrogen deficiency alters bone quality. It was observed that, compared to bone from other anatomical sites, the jaw bone is less sensitive to estrogen deficiency. Thus, the objective of this study was to examine the effect of estrogen deficiency on 1) the regional variations of tissue modulus and viscosity of bone using nanoindentation, and 2) the modulus-viscosity relationships in jaw and vertebral bones for comparison between different anatomical sites. Mandibular and vertebral bone specimens of sham surgery and ovariectomized (OVX) rat groups were subject to nanoindentation in hydration. Indentation modulus and viscosity were measured at relatively new (less mineralized) tissue regions and at the corresponding pre-existing old (more mineralized) tissue regions of mandibular and vertebral bones. In the mandibular bones, significant regional variations of indentation modulus and viscosity were observed (p<0.039) and OVX increased the indentation viscosity. While significant positive correlations were found between indentation modulus and viscosity (p<0.001), the correlation slopes for the mandibular and vertebral bones were significant different (p<0.001). The current results indicated that changes in viscoelastic property and its regional variation should be examined to obtain a better understanding of estrogen deficiency-dependent alteration of bone quality.

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“…Recent research has confirmed that Ovx leads to a significant reduction of bone mass in femurs and tibia, which are formed by endochondral ossification, but not in maxilla, which is formed by intramembranous ossification [ 12 ]. Most studies have tended to focus on the bone loss in long bone and mandibular alveolar bone after Ovx, using three-dimensional (3D) micro-computed tomography (µCT) [ 8 ], dual-energy X-ray absorptiometry (DEXA) [ 13 ], and Raman spectroscopy [ 14 ]; however, the effects of Ovx on the bone remodelling in maxilla and tibia remain unclear.…”

Section: Introductionmentioning

confidence: 99%

Multi-Elemental Profiling of Tibial and Maxillary Trabecular Bone in Ovariectomised Rats

Han

Zhou

et al. 2016

IJMS

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Atomic minerals are the smallest components of bone and the content of Ca, being the most abundant mineral in bone, correlates strongly with the risk of osteoporosis. Postmenopausal women have a far greater risk of suffering from OP due to low Ca concentrations in their bones and this is associated with low bone mass and higher bone fracture rates. However, bone strength is determined not only by Ca level, but also a number of metallic and non-metallic elements in bone. Thus, in this study, the difference of metallic and non-metallic elements in ovariectomy-induced osteoporosis tibial and maxillary trabecular bone was investigated in comparison with sham operated normal bone by laser ablation inductively-coupled plasma mass spectrometry using a rat model. The results demonstrated that the average concentrations of 25Mg, 28Si, 39K, 47Ti, 56Fe, 59Co, 77Se, 88Sr, 137Ba, and 208Pb were generally higher in tibia than those in maxilla. Compared with the sham group, Ovariectomy induced more significant changes of these elements in tibia than maxilla, indicating tibial trabecular bones are more sensitive to changes of circulating estrogen. In addition, the concentrations of 28Si, 77Se, 208Pb, and Ca/P ratios were higher in tibia and maxilla in ovariectomised rats than those in normal bone at all time-points. The present study indicates that ovariectomy could significantly impact the element distribution and concentrations between tibia and maxilla.

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Effects of Ovariectomy on Rat Mandibular Cortical Bone: A Study Using Raman Spectroscopy and Multivariate Analysis

Cited by 12 publications

References 40 publications

Polarization in Raman spectroscopy helps explain bone brittleness in genetic mouse models

Polarization in Raman spectroscopy helps explain bone brittleness in genetic mouse models

Effect of estrogen deficiency on regional variation of a viscoelastic tissue property of bone

Multi-Elemental Profiling of Tibial and Maxillary Trabecular Bone in Ovariectomised Rats

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