Aim: To evaluate the shear bond strength (SBS) of 3 fissure sealants used alone and with ozone and laser pretreatment. Materials and Methods: For the SBS test, the flattened enamel surfaces of 90 healthy third molars were randomly divided into nine groups ( n = 10 for each groups), based on different sealants (BeautiSealant, Fuji Triage, and Grandio Seal) and pretreatment conditions (no pretreatment, pretreatment with ozone, and pretreatment with neodymium-doped yttrium aluminum garnet [Nd:YAG laser]). Ozone was applied for 30 s in caries superficial mode, and Nd:YAG laser was applied for 60 s. After the pretreatment procedures, sealants were placed inside a mold on the enamel surface. SBS was measured with a universal testing machine. Data were analyzed using analysis of variance. Tukey’s multiple comparison tests were applied to determine the difference between the groups (α = 0.05). Results: The lowest SBS was observed in the Fuji triage group. Nd:YAG laser and ozone did not affect the SBS of BeautiSealant. However, significant differences were determined in the Fuji triage and Grandio Seal groups after pretreatment procedures ( P < .05). Conclusion: Ozone and laser can be employed to disinfect fissures before BeautiSealant application.