Effects of partially replacing glycerol with cholesterol-loaded cyclodextrin on protamine deficiency, in vitro capacitation and fertilization ability of frozen–thawed Yanbian Yellow cattle sperm

Lv, Yanqiu; Jin, Qingguo; Chen, Xuan; Wang, Shi; Luo, Xi; Han, Yang; Cheng, Min-Min; Qu, Xingling; Zhang, Yuyang; Jin, Yi

doi:10.1016/j.theriogenology.2021.12.008

Cited by 5 publications

(3 citation statements)

References 40 publications

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“…A detailed analysis of post thaw motilities across mammalian species comparisons was conducted but did not yield any valuable insights due to the considerable variation between studies in the protocols and media employed as well as inter-species variation in the susceptibility of the spermatozoa to cryodamage (Purdy and Graham 2004;Moce et al 2010;Oldenhof et al 2013;Ghallab et al 2017;Lee and Kim 2018;Lv et al 2022). Although multiple tests were done for each variation, motility was the only result shared by all publications (Table 4).…”

Section: Animal Studiesmentioning

confidence: 99%

“…Which species is used for each CPA is shown in Table 4. (Purdy and Graham 2004;Moce et al 2010;Oldenhof et al 2013;Ghallab et al 2017;Lee and Kim 2018;Lv et al 2022).…”

Section: Reproduction Fertility and Developmentmentioning

confidence: 99%

“…Comparison of animal-focused papers demonstrating freezing method and total motility results (Purdy and Graham 2004;. Moce et al 2010;Oldenhof et al 2013;Ghallab et al 2017;Lee and Kim 2018;Lv et al 2022).…”

mentioning

confidence: 99%

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Sperm cryopreservation: current status and future developments

Hungerford¹,

Bakos²,

Aitken³

2022

Reprod. Fertil. Dev.

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The cryopreservation of spermatozoa is an important reproductive technology for the preservation of fertility in man and animals. Since the serendipitous discovery of glycerol as an effective cryoprotectant in 1947, sperm cryopreservation has undergone many changes in terms of the freezing methods employed, the rates at which samples are frozen and thawed, and the media used to preserve sperm functionality and DNA integrity. An extensive literature survey has been conducted addressing the cryoprotectants employed for both animal and human semen and the freezing protocols utilised. The results indicate that glycerol remains the dominant cryoprotective agent, usually incorporated into a balanced salt solution containing energy substrates, buffers, osmolytes and protein in the form of human serum albumin (human) or skimmed milk (animal). Realisation that some of the damage observed in cryostored cells involves the generation of reactive oxygen species during the thawing process, has prompted many studies to assess the relative merits of incorporating antioxidants into the cryopreservation media. However, in the absence of systematic comparisons, there is currently no consensus as to which antioxidant combination might be the most effective. Utilising our fundamental understanding of cryodamage to optimise cryopreservation protocols for each species will be important in the future.

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Section: Animal Studiesmentioning

confidence: 99%

“…Which species is used for each CPA is shown in Table 4. (Purdy and Graham 2004;Moce et al 2010;Oldenhof et al 2013;Ghallab et al 2017;Lee and Kim 2018;Lv et al 2022).…”

Section: Reproduction Fertility and Developmentmentioning

confidence: 99%