Effects of pentoxifylline administration on histomorphological parameters of streptozotocin-induced diabetic rat testes

Abstract

The effect of pentoxifylline (PTX) administration on histomorphological parameters of streptozotocin (STZ)-induced type 1 diabetes mellitus (DM) in male rat testes were evaluated. We randomly divided 40 male rats into the following four groups: group 1: control or normal glycemic (NG) rats; group 2 or NG rats that received only normal saline (NS), (NG+NS); group 3 or diabetic rats which were not treated by PTX (DM+vehicle solution (NS)); and group 4 which comprised diabetic rats treated with 50 mg/kg of PTX (DM+PTX). Type 1 DM was induced by intraperitoneal injection of STZ (55 mg/kg). Rats were held for 30 days after which the experimental group received PTX twice daily (25 mg/kg) or NS. After 14 days of treatment by PTX or NS, the left testes from all rats were extracted and prpared for histological study. Apoptotic cells, blood vessel density, and spermatogenesis were evaluated. Data were analyzed by ANOVA test. PTX-treated-diabetic rats showed a significant decrease in number of apoptotic cells and decrease in blood vessel density compared to the DM+NS rats. A significant increase in spermatogenesis was observed in the PTX-treated diabetic group, compared to the DM+NS groups. It was concluded that PTX administration to STZ-induced type 1 DM rats affected apoptotic cell number positively. Moreover, blood vessel density significantly decreased and improvements were observed in spermatogenesis.

Therapeutic effect of pentoxifylline on reproductive parameters in diabetic male mice

“…In other words, we quantitatively determined that PTX exerts its therapeutic effect on seminiferous tubule architecture. Furthermore, present study followed the fate of produced cells of testis, spermatozoa, evaluated sugar and testosterone blood serum levels in diabetic mice, rather than that study (Piryaei et al ., ), confirming the efficacy of PTX for both reliving the dangerous effect of diabetes on male reproductive system and decreasing the high blood glucose of diabetic mice. The study also showed that PTX inhibits cell death in testis of diabetic mice as the same as Piryaei and colleagues have shown (Piryaei et al ., ) and the antioxidant properties of PTX may explain this status as follows.…”

“…Recently, Piryaei et al . () showed that PTX improves spermatogenesis of diabetic rats by evaluating Johnson score. To achieve more reliable data, we examined a quantitative method, evaluation of seminiferous diameter, to show the effects of PTX on histopathological changes due to diabetes.…”

Effects of <i>Vernonia cinerea</i> on reproductive performance in streptozotocin-induced diabetic rats

“…The prevalence of DM has increased rapidly. The World Health Organization (WHO) has estimated that the number of patients with DM is anticipated to progress to 366 million by 2030 [5, 26, 41]. DM can result in many systemic complications, such as complication in the heart, kidneys, peripheral nerves and reproductive system [5, 9].…”

Two months sodium nitrate supplementation alleviates testicular injury in streptozotocin‐induced diabetic male rats

“…In line with our findings, Piryaei et al . showed through evaluating Johnsen's score that diabetes impaired spermatogenesis of diabetic rats (Piryaei, Najar, & Bayat, ). Sodium nitrite or insulin treatment resulted in an increased Johnsen's score toward that of controls.…”