Effects of pH on the growth rate, motility and photosynthesis inEuglena gracilis

Danilov, Roman A.; Ekelund, Nils

doi:10.1007/bf02818001

Cited by 31 publications

(19 citation statements)

References 25 publications

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“…Above pH 6 the growth rate shows an inhibition which looks similar to the effects of high pH values on the motility parameters of E. gracilis. Similar results were shown in a study by Danilov and Ekelund [46]; cells of E. gracilis below pH 4 and above pH 8 did not survive.…”

supporting

confidence: 78%

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Environmental monitoring using bioassays

Ekelund

Häder

2018

Bioassays

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supporting

confidence: 78%

“…Copper (Cu) is an essential microelement and has also been shown to have toxic effects at higher concentrations on different parameters in E. gracilis [26,46], The immediate response to copper was rapid when studying motility parameters with ECOTOX [26]. Already after 3 min copper showed a disturbance of the different motility parameters.…”

mentioning

confidence: 99%

Environmental monitoring using bioassays

Ekelund

Häder

2018

Bioassays

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“…The medium was modified by addition of ethanol to 30 mM as a carbon source [2], CoCl 2 (1.3 mg/l) and Na 2 MoO 4 (0.2 mg/l) in place of Co(NO 3 ) 2 and H 2 MoO 4 , respectively, and adjusted to pH 6.5 with phosphoric acid [86]. Cultures were harvested at mid- to late-log phase (after 4-5 days of growth), at an OD 600 between 0.8 and 1.0.…”

Section: Methodsmentioning

confidence: 99%

U3 snoRNA genes are multi-copy and frequently linked to U5 snRNA genes in Euglena gracilis§

Charette

Gray

2009

BMC Genomics

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BackgroundU3 snoRNA is a box C/D small nucleolar RNA (snoRNA) involved in the processing events that liberate 18S rRNA from the ribosomal RNA precursor (pre-rRNA). Although U3 snoRNA is present in all eukaryotic organisms, most investigations of it have focused on fungi (particularly yeasts), animals and plants. Relatively little is known about U3 snoRNA and its gene(s) in the phylogenetically broad assemblage of protists (mostly unicellular eukaryotes). In the euglenozoon Euglena gracilis, a distant relative of the kinetoplastid protozoa, Southern analysis had previously revealed at least 13 bands hybridizing with U3 snoRNA, suggesting the existence of multiple copies of U3 snoRNA genes.ResultsThrough screening of a λ genomic library and PCR amplification, we recovered 14 U3 snoRNA gene variants, defined by sequence heterogeneities that are mostly located in the U3 3'-stem-loop domain. We identified three different genomic arrangements of Euglena U3 snoRNA genes: i) stand-alone, ii) linked to tRNAArg genes, and iii) linked to a U5 snRNA gene. In arrangement ii), the U3 snoRNA gene is positioned upstream of two identical tRNAArg genes that are convergently transcribed relative to the U3 gene. This scenario is reminiscent of a U3 snoRNA-tRNA gene linkage previously described in trypanosomatids. We document here twelve different U3 snoRNA-U5 snRNA gene arrangements in Euglena; in each case, the U3 gene is linked to a downstream and convergently oriented U5 gene, with the intergenic region differing in length and sequence among the variants.ConclusionThe multiple U3 snoRNA-U5 snRNA gene linkages, which cluster into distinct families based on sequence similarities within the intergenic spacer, presumably arose by genome, chromosome, and/or locus duplications. We discuss possible reasons for the existence of the unusually large number of U3 snoRNA genes in the Euglena genome. Variability in the signal intensities of the multiple Southern hybridization bands raises the possibility that Euglena contains a naturally aneuploid chromosome complement.

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“…The cells were cultivated statistically on a modified Cramer-Myers medium (Danilov and Ekelund 2001) supplemented with 0.5% sodium acetate at pH 6.8 at 26 + 28C under permanent illumination.…”

Section: Reagentsmentioning

confidence: 99%

Detection of the Effective DNA Protection by Quinazolines Using a DNA‐Based Electrochemical Biosensor

2005

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A DNA biosensor can serve as a powerfull tool during simple in vitro tests of chemical toxicity. In this paper, damage to dsDNA attached to the surface of a screen-printed carbon electrode by acridine orange (AO) in 5 Â 10 23 M phosphate buffer solution and an effective DNA protection by selected quinazolines are described. Damage to DNA was detected using voltammetric signals of the dsDNA guanine moieties. Using the Co(III) complex with 1,10-phenanthroline, [Co(phen) 3 ] 3þ , as an electrochemical DNA marker, the portion of original dsDNA that survives an incubation of the biosensor in the medium of AO and quinazolines was evaluated. The quinazoline derivatives under study were found to be very effective DNA protectors, particularly at low concentration level. The results were confirmed by microbiological tests with green seaweed, Euglena gracilis, as well as by conventional electrophoretic experiments.

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Effects of pH on the growth rate, motility and photosynthesis inEuglena gracilis

Cited by 31 publications

References 25 publications

Environmental monitoring using bioassays

Environmental monitoring using bioassays

U3 snoRNA genes are multi-copy and frequently linked to U5 snRNA genes in Euglena gracilis§

Detection of the Effective DNA Protection by Quinazolines Using a DNA‐Based Electrochemical Biosensor

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