Effects of phosphonoformic acid and 1‐hydroxyethylidene‐1, 1‐bisphosphonate (HEBP) on distribution of tetracycline in the developing rat molar

Abstract

Effects of phosphonoformic acid and 1-hydroxyethylidene-1,1-bisphosphonate(HEBP)on , . distribution of tetracycline in the developing rat molar Fouda N, Kttt I A. Hammarstrom L: Effects of phosphonoformic acid and l-hydroxyethylidene-l,l-bisphosphonate (HEBP) on distribittion of tetracycline in the developing rat tnolar. Scand J Dent Res 1992; 100; 257-65. Tetracycline was used as a marker to study the effect of phosphonoformic acid and l-hydroxyethylidene-l,l-bisphosphonate (HEBP) on the mineralization of the developing dental hard tissues. Groups of young rats were given a single subcutaneous injection of tetracycline and at the same time were injected with a single dose of either phosphonoformic acid or HEBP (10 mg P/kg b.w.). Alternatively, rats were injected with the tetracycline after different time intervals from the phosphonate injection. Rats were sacrificed at intervals ranging from 1 to 4 days. Frozen-sections were obtained at the level of the maxillary first molar and prepared for light and fluorescence microscopy. The results of the present study indicate that the distribution pattern of tetracycline in the developing dental hard fissues is greatly affected by the pathologic changes induced by phosphonoformic acid and HEBP. Both drugs caused similar changes in the pattern of tetracycline uptake in the developing enamel. There seems to be a direct relation between the presence of developmental defects of enamel and the degree of discoloration induced by tetracycline. Aberrations in the mineralization of dentin were caused only by HEBP. As demonstrated by this study, HEBP is capable of inducing a provisional inhibitory effect on dentin mineralization.

Disturbances of cementum formation induced by single injection of 1‐hydroxyethylidene‐1,1‐bisphosphonate (HEBP) in rats: light and scanning electron microscopic studies

“…It has long been known that mineralization of the mantle dentin is a requirement for the differentiation of ameloblasts (5,6,10,18). This has been confirmed more recently in studies where HEBP was injected (9,(15)(16)(17). The mechanisms by which mineralization of the mantle dentin induces differentiation and phenotypic expression of adjacent cells remain to be defined.…”

Systemically alendronate was incorporated into dental tissues but did not cause morphological or mechanical changes in rats teeth

“…For instance, the effects of etidronate (HEBP), a nonaminobisphosphonate, on collagen fibrillogenesis, matrix synthesis, and mineralization of dentin have been reported (Beertsen et al,1985; Larsson,1974; Ogawa et al,1989; Sakai et al,1999; Takano et al,1998). HEBP has also been shown to affect enamel mineralization (Espírito Santo et al,2010; Fouda et al,1989, 1991, 1992; Weile et al,1990, 1993), ameloblast morphology (Fejerskov et al,1990; Josephsen et al,1990), and ameloblast functions, including effects on protein synthesis and secretion (Fuangtharnthip et al,2000; Yamada et al,2000), with development of hypoplasia and formation of subameloblastic cysts (Fejerskov et al,1990; Fouda et al,1989, 1991, 1992; Simmelink,1987). …”

Efeito do uso sistêmico de alendronato sódico no tecido ósseo e nas estruturas dentárias mineralizadas: estudo químico, mecânico e morfológico, em modelo murino

“…A administração de injeção única de etidronato em ratos induziu uma banda hipomineralizada na camada de esmalte (Ogawa et al, 1989;Josephsen et al, 1990;Fouda et al, 1989;1992). Segundo Fouda et al (1991), o exame microrradiográfico de molares de ratos revelou uma faixa de hipomineralização na matriz do esmalte, correspondente à superfície externa em desenvolvimento, no momento da injeção do etidronato.…”

“…Adicionalmente, foi demonstrado que o tratamento com alguns BFs não aminados, após administração de doses únicas ou múltiplas, interferiu na amelogênese, causando distúrbios nos ameloblastos e, consequentemente, no desenvolvimento do esmalte, incluindo hipoplasias e formação de cistos subameloblásticos (Simmelink, 1987;Fouda et al, 1989;1992;Fejerskov et al, 1990). Segundo Fouda et al (1989) (Fouda et al, 1989;.…”

“…Estudos microscópicos em ratos demonstraram que o tratamento com alguns BFs, como o etidronato, interfere na amelogênese, ocasionando distúrbios nos ameloblastos e, consequentemente, no desenvolvimento do esmalte, incluindo hipomineralização e formação de cistos subameloblásticos (Simmelink, 1987;Fouda et al, 1989;1992;Fejerskov et al, 1990). …”