This research aimed to verify the influence of the photoperiod on the performance and survival piabanha-of-Pardo (Brycon vonoi) larvae. Piabanha-of-Pardo larvae 24 hours post-hatch, with body weight of 2.44 ± 0.02mg, and total length of 7.82 ± 0.46mm, were subjected to five different photoperiods: 0L:24D, 6L:18D, 12L:12D, 18L:6D and 24L:0D (871.12 ± 92.65 lux). Daily, the water temperature, dissolved oxygen (DO), percentage of oxygen saturation, pH, and water conductivity were monitored, and presented similar water quality values among tanks with distinct photoperiods, and within the range considered appropriate for the genus Brycon larvae. Piabanha-of-Pardo larvae have better productive performance and survival rate at longer photoperiods, over 6 hours of light and below 12 hours of light per day. The 9L:15D photoperiod, specified by the derived equation, is indicated for the piabanha larviculture, but researches with the species and the photoperiod need to be better studied.