Effects of Phytoremediation on Microbial Biomass, Composition, and Function in a Sulphide-Rich Tailing From a Metal-Contaminated Region

Abstract

Mining activities lead to serious land deterioration and large scale mine waste generation. Reclamation has been carried out on several technogenic materials to encourage the development of soils. To date no detailed studies have been conducted to assess if soil developed in reclaimed tailings can be suitable for microbial community sustainability and associated plant population. This study investigated if 1) soil metal contamination affects microbial biomass and composition in sulphide tailings and 2) phytoremediation of tailing increases microbial abundance, diversity, and function. Microbial biomass was assessed using Phospholipid fatty acid analysis (PLFA). Soil bacterial and fungal microbiota was determined by high throughput sequencing of 16S rRNA gene for bacteria and internal transcribed spacer region for fungi using the Illumina platform. Total copper, nickel, iron, and titium were higher in unreclaimed sites compared to vegetated areas but the total microbial biomass was significantly higher in reclaimed sites compared to reference areas. More importantly, the levels of microbial biomass were not impacted by metals since the bioavailable Cu, Ni, and Ti were low in all the sites. Site-specific bacterial and fungal genera were identified. Proteobacteria was the most dominant bacterial phylum while Ascomicota was the predominant fungal phylum. Interestinlgy, Acidiferrobacter, an acidophilic, thermotolerant and facultatively anaerobic was the most predominant genus in unreclaimed site that is characterized by extreme acidity (pH = 2.8). Analysis of microbial diversity revealed higher Chao 1, # of OTUs, Shannon index, and species richness in bacterial and fungal populations from reclaimed sites compared to controls. The levels of β-glucosidase (BG), cellobiohydrolase (CBH), β-N-acetylglucosaminidase (NAGase), aryl sulfatase (AS), acid phosphatase (AP), alkaline phosphatase (AlP), glycine aminopeptidase (GAP), and leucine aminopeptidase (LAP) activities were significantly higher in vegetated sites compared to reference areas. Strong positive correlation coefficients were observed between soil organic matter and total microbial biomass (r = 0.99). These two factors were positively correlated with enzymatic activities and bacterial population diversity. Overall, newly developed soils can sustain diverse microbial communities and associated vegetations.

Levels of Metals and Microbial Biomass in Cobalt Coleman Mine Tailings (Canada) Three Decades after Land Reclamation

“…Several studies have documented a strong positive correlation between Microbial Biomass Carbon (MBC) and Soil Organic Carbon (SOC) (Narendrula-Kotha and Nkongolo 2017a-b;Dimitriu and Grayston, 2010;Li et al, 2016;McKergow et al, 2021;Nkongolo et al, 2022b). Data from this study are consistent with previous findings since higher microbial biomass in sites was associated with high organic matter.…”

“…They reported no significant differences for # of OTUs, Chao 1, Simpson index, Shannon index, and species richness among the bacterial communities from reclaimed tailing areas in the City of Greater Sudbury (CGS) (Ontario). On the other hand, Nkongolo et al (2022b) highlighted a higher # of OTUs, Chao 1, Shannon index, and species richness in microbial populations from newly reclaimed tailing sites compared to reference areas.…”

“…Soil samples were analyzed following the protocol described by Buyer and Sasser (2012); Narendrula-Kotha and Nkongolo (2017a-b). A summary of this method is also reported in Nkongolo et al, (2022b). 38.353', W79° 68.457' and N47° 23.466', W79° 40.797', for Cart Lake and Nipissing tailings, respectively.…”