Effects of Platelet-Rich Plasma on the Healing of Autologous Bone Grafted Mandibular Defects in Dogs

Gérard, David; Carlson, Eric R.; Gotcher, Jack E.; Jacobs, Mykle

doi:10.1016/j.joms.2005.11.016

Cited by 97 publications

(93 citation statements)

References 15 publications

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“…Some studies have demonstrated that PRP enhances only the early healing of autogenous bone grafts. 11,33 Mandibular defects surgically created in dogs and treated with autogenous bone grafts combined with PRP presented a greater volume of new bone than defects treated with autogenous bone grafts at 4 and 8 weeks postoperative. However, this effect was no longer significant at 12 weeks postoperative.…”

Section: Discussionmentioning

confidence: 97%

“…However, this effect was no longer significant at 12 weeks postoperative. 11 The controversies regarding the influence of PRP on wound healing should be reexamined considering many variables, including the ratio of particulate autogenous bone graft/PRP. According to Choi et al, 19 a dilution of the PRP concentrations to a level suitable for cell proliferation may be necessary for use in bone grafts to enhance bone cell growth within the PRP-treated bone graft.…”

Section: Discussionmentioning

confidence: 99%

“…1 Although some found significant increases in bone formation and maturation rates, 1,[5][6][7] others did not observe any improvement. [8][9][10][11] Such discrepancies probably relate to the lack of standardization of PRP preparation protocols. 12 The protocols and surgical techniques used in preparing and administering PRP differ widely, 13,14 and variations can greatly influence PRP's biological effects.…”

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confidence: 99%

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Influence of the ratio of particulate autogenous bone graft/platelet-rich plasma on bone healing in critical-size defects: A histologic and histometric study in rat calvaria

et al. 2009

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The purpose of this study was to analyze histomorphometrically the influence of the ratio of particulate autogenous bone (AB) graft/platelet-rich plasma (PRP) on bone healing in surgically created critical-size defects (CSD) in rat calvaria. Fifty rats were divided into five groups: Group C (control), Group AB, Group AB/PRP-50, Group AB/PRP-100, and Group AB/PRP-150. A 5-mm diameter critical-size defect was created in the calvarium of each animal. In Group C, the defect was filled by blood clot only. In Group AB, the defect was filled with 0.01 mL of AB graft. In Groups AB/PRP-50, AB/PRP-100, and AB/PRP-150, the defects were filled with 0.01 mL of AB graft combined with 50, 100, and 150 mL of PRP, respectively. All animals were euthanized at 30 days postoperative. Histomorphometry, using image analysis software, and histology analyses were performed. New Bone Area (NBA) and the remaining bone graft particles area (RPA) were calculated as a percentage of the total area of the original defect. Percentage data were transformed into arccosine for analysis. No defect completely regenerated with bone. Group AB/PRP-50 (41.78 AE 13.48%) had a significantly greater NBA than Groups C (19.29 AE 5.11%), AB (27.43 AE 10.90%) or AB/PRP-150 (19.17 AE 8.45%) (p < 0.05). No significant differences were observed between groups AB/PRP-50 and AB/ PRP-100 or among groups AB, AB/PRP-100, and AB/PRP-150 with regard to NBA (p > 0.05). Group AB/PRP-150 (31.59 AE 3.22%) had a significantly greater RPA than Groups AB (19.09 AE 5.21%), AB/PRP-50 (17.33 AE 4.43%), and AB/PRP-100 (19.72 AE 3.62%) (p < 0.001). No significant differences were observed among groups AB, AB/PRP-50, and AB/PRP-100 with regard to RPA (p > 0.05). The ratio AB graft/PRP influences bone healing in surgically created CSD in rat calvaria. ß

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Influence of the ratio of particulate autogenous bone graft/platelet-rich plasma on bone healing in critical-size defects: A histologic and histometric study in rat calvaria

et al. 2009

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“…It has the additional advantage of using the host as a donor source, which avoids bone graft donor site morbidity. Animal studies have yielded variable results (Gandhi et al, 2006;Gerard et al, 2006;Sarkar et al, 2006). Unfortunately, clinical studies have not seen the same success with the use of PRP as has been seen with other osteoinductive products such as BMPs (Carreon et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…Unfortunately, clinical studies have not seen the same success with the use of PRP as has been seen with other osteoinductive products such as BMPs (Carreon et al, 2005). This lack of success may be due to the relatively low concentration of growth factors that can be extracted in this mannerdonly about five times the concentration of plasma (Gerard et al, 2006) compared with the 10-fold (Carreon et al, 2005) increase in synthetic BMPs (Westerhuis et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

The role of bone marrow aspirate cells in the management of atrophic mandibular fractures by mini-invasive surgical approach: Single-institution experience

Mannelli

Arcuri

Conti

et al. 2017

Journal of Cranio-Maxillofacial Surgery

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Elderly patients management Bone marrow aspirate cells Min-invasive surgical approach a b s t r a c t Purpose: The treatment of fractures involves addressing the biology of fracture repair and the mechanical stability of fracture fixation. Traditionally it has included the addition of bone graft to enhance healing. New advances in the understanding of the cellular and molecular mechanisms of fracture repair have led to the use of growth factors to accelerate bone healing. This study aimed to assess the advantages of autologous stem cell use for atrophic mandibular fracture treatment in comparison to standard technique. Materials and methods: A total of 35 patients (14 male and 21 female) were treated for fractures of atrophic mandibles between January 2011 and December 2014. Surgical technique provided miniinvasive open reduction with or without immediate homologous bone graft, while selected patients received autologous bone marrow aspirate cell grafts in addition to the standard treatment. Demographic data and details of treatment and outcomes were recorded. Results: Patients were categorized according to the use of autologous stem cells, leaving 17 patients treated with standard technique and autologous stem cells (Group A) and 18 treated with standard osteosynthesis only (Group B). Of the 35 patients, 26 had bilateral fractures; most patients had significant medical co-morbidities. Immediate bone graft was used in 37.1% of patients (7 in group A and 6 in group B). Complications occurred in 5 patients (14.3%). Two patients in group B (11.1%) showed non-union of the fracture. One patient in group A (5.8%) and one patient in group B (5.5%) showed wound dehiscence and were treated conservatively; one patient in group B had a local infection (5.5%), one out of 35 (2.8%), that was managed by prolonged antibiotic treatment. Conclusions: Despite the advanced age and medical co-morbidities of the vast majority of patients, miniinvasive open approach with autologous bone graft ensures a fast and excellent recovery. Moreover, the management of atrophic mandibular fractures by bone marrow aspirate cells is a safe and useful procedure which has a lower complication rate when compared to standard technique.

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Osteogenic properties of starch poly(ε‐caprolactone) (SPCL) fiber meshes loaded with osteoblast‐like cells in a rat critical‐sized cranial defect

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Gardel

Correlo

et al. 2013

J Biomedical Materials Res

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Osteoblast-like cells together with a suitable scaffold can aid to the regeneration of bone defects. A suitable scaffold could be starch poly(ε-caprolactone) (SPCL) fiber meshes, which have shown a high potential to support bone formation in previous in vitro and in noncritical sized in vivo studies. The aim of this study was to assess the effect of these scaffolds alone or combined with osteoblast-like cells in the regeneration of a critical-sized cranial defect in male Fisher rats. Empty defects and defects filled with cell-free scaffolds were used as controls groups. Samples were analyzed by microcomputed tomography (micro-CT) and histological analyses. Histological analyses revealed that all study groups showed new bone formation from the defect edges toward the interior of the defects. In addition, bone was formed in the center of the scaffolds, especially in the groups containing preloaded osteoblast-like cells. Micro-CT reconstructions showed that bone formation increased over time and was enhanced with the inclusion of preloaded osteoblast-like cells compared with SPCL scaffolds alone. According to these results, the preloaded osteoblast-like cells contributed to the bone regeneration process in a critical-sized bone defect. Furthermore, SPCL fiber meshes proved to be an osteoconductive material to use for bone regeneration purposes.

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Effects of Platelet-Rich Plasma on the Healing of Autologous Bone Grafted Mandibular Defects in Dogs

Cited by 97 publications

References 15 publications

Influence of the ratio of particulate autogenous bone graft/platelet-rich plasma on bone healing in critical-size defects: A histologic and histometric study in rat calvaria

Influence of the ratio of particulate autogenous bone graft/platelet-rich plasma on bone healing in critical-size defects: A histologic and histometric study in rat calvaria

The role of bone marrow aspirate cells in the management of atrophic mandibular fractures by mini-invasive surgical approach: Single-institution experience

Osteogenic properties of starch poly(ε‐caprolactone) (SPCL) fiber meshes loaded with osteoblast‐like cells in a rat critical‐sized cranial defect

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