Effects of polycyclic aromatic hydrocarbons on liver and lung cytochrome P450s in Mice

Abstract

Certain polycyclic aromatic hydrocarbons (PAHs) have been reported to induce cytochrome P450 (CYP) 1A1 and 1A2. In the present study, the effects of six well-known PAHs, such as benzo[a]pyrene, benz[a]anthracene, dibenz[a,h]anthracene, chrysene, benzo[k]fluorancene and benzo[b]fluorancene, on the activities of hepatic and pulmonary CYP enzymes were investigated in male ICR mice. When mice were treated intraperitoneally with 3, 10 and 30 mg/kg of individual PAHs for 3 consecutive days, the activities of ethoxyresorufin- and methoxyresorufin-O-dealkylases were significantly and differentially induced in both liver and lung. Moreover, other CYP isozyme-associated monooxygenase activities were also induced significantly in liver and lung with characteristic induction profiles. Our present results suggest that individual PAHs might have inductive effects on CYP isozymes, and that the characteristic inductive effects of individual PAHs on certain CYP isozymes would be developed as a marker for determining exposure to certain PAHs.

In vitro genotoxic and mutagenic potentials of combustion particles from marine fuels with different sulfur contents

“…The relatively high concentration of chrysene, abundant in ULS-HFO AR , and HS-HFO SYN particles, may contribute to the significant induction of CYP1A1/1A2, as chrysene has been shown to induce significant activation of CYP1A1 and 1A2 in mouse liver cells (Shimada and Fujii-Kuriyama, 2004). Moreover, the presence of BaP and benz[a]anthracene is attributed to the increase of EROD activity, as shown in a previous in vivo study (Kim et al, 2003). The PM from aromatic-rich fuel types showed a nonsignificant, but still PM concentration-related induction of BROD.…”

Some outcomes and a hypothetical mechanism of combined lead and benzo(a)pyrene intoxication, and its alleviation with a complex of bioprotectors

“…These transformations are controlled by so-called microsomal mixed-function oxidases. The central place in this poly-enzyme complex belongs to hemoprotein enzymes, among which cytochrome P450 plays a major role [ [16] , [17] , [18] ].…”