Effects of potential probiotic Bacillus subtilis T13 on growth, immunity and disease resistance against Vibrio splendidus infection in juvenile sea cucumber Apostichopus japonicus

Zhao, Yancui; Zhang, Wenbing; Xu, Wei; Mai, Kangsen; Zhang, Yanjiao; Liufu, Zhiguo

doi:10.1016/j.fsi.2012.01.027

Cited by 174 publications

(101 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…If significant differences were found (P o0.05), Duncan's multiple range tests were used to rank the groups [65]. All statistical analyses were performed using the SPSS 13.0 for Window software (SPSS, Chicago, IL, USA) [66]. 50 ) on the activities of caspase-8, caspase-9 and caspase-3 were respectively estimated to be 0.60, 0.61 and 0.35 mM that are the minimum values among amino acids examined so far (see Table 1 in Ref.…”

Section: Protein Measurements and Statistical Analysismentioning

confidence: 99%

The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes

Jiang

Liu

et al. 2016

Free Radical Biology and Medicine

View full text Add to dashboard Cite

a b s t r a c tThe present study explored the apoptosis pathways in hydroxyl radicals ( • OH)-induced carp erythrocytes. Carp erythrocytes were treated with the caspase inhibitors in physiological carp saline (PCS) or Ca 2 þ -free PCS in the presence of 40 μM FeSO 4 /20 μM H 2 O 2 . The results showed that the generation of reactive oxygen species (ROS), the release of cytochrome c and DNA fragmentation were caspase-dependent, and Ca 2 þ was involved in calpain activation and phosphatidylserine (PS) exposure in • OH-induced carp erythrocytes. Moreover, the results suggested that caspases were involved in PS exposure, and Ca 2 þ was involved in DNA fragmentation in • OH-induced fish erythrocytes. These results demonstrated that there might be two apoptosis pathways in fish erythrocytes, one is the caspase and cytochrome c-dependent apoptosis that is similar to that in mammal nucleated cells, the other is the Ca 2 þ -involved apoptosis that was similar to that in mammal non-nucleated erythrocytes. So, fish erythrocytes may be used as a model for studying oxidative stress and apoptosis in mammal cells. Furthermore, the present study investigated the effects of glutamine (Gln)'s metabolites [alanine (Ala), citrulline (Cit), proline (Pro) and their combination (Ala 10 Pro 4 Cit 1 )] on the pathways of apoptosis in fish erythrocytes. The results displayed that Ala, Cit, Pro and Ala 10 Pro 4 Cit 1 effectively suppressed ROS generation, cytochrome c release, activation of caspase-3, caspase-8 and caspase-9 at the physiological concentrations, prevented Ca 2 þ influx, calpain activation, PS exposure, DNA fragmentation and the degradation of the cytoskeleton and oxidation of membrane and hemoglobin (Hb) and increased activity of anti-hydroxyl radical (AHR) in • OH-induced carp erythrocytes. Ala 10 Pro 4 Cit 1 produced a synergistic effect of inhibited oxidative stress and apoptosis in fish erythrocytes. These results demonstrated that Ala, Cit, Pro and their combination can protect mammal erythrocytes and nucleated cells against oxidative stress and apoptosis. The studies supported the use of Gln, Ala, Cit and Pro as oxidative stress and apoptosis inhibitors in mammal cells and the hypothesis that the inhibited effects of Gln on oxidative stress and apoptosis are at least partly dependent on that of its metabolites in mammalian.

show abstract

Section: Protein Measurements and Statistical Analysismentioning

confidence: 99%

The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes

Jiang

Liu

et al. 2016

Free Radical Biology and Medicine

View full text Add to dashboard Cite

show abstract

“…The IgM contents were assayed using a IgM contents kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) following the manufacturer's protocols. The ACP activity and C3, C4 content were assayed according to Wu et al [60], Zhao et al [61] and Yin et al [62], respectively. The reactive oxygen species (ROS) content of the head kidney, spleen and skin was measured using 2 0 7 0 -dichlorodihydrofluorescein diacetate which was oxidized to fluorescent dichlorofluorescein (DCF) by ROS.…”

Section: Biochemical Analysismentioning

confidence: 99%

Dietary low or excess levels of lipids reduced growth performance, and impaired immune function and structure of head kidney, spleen and skin in young grass carp (Ctenopharyngodon idella) under the infection of Aeromonas hydrophila

Jiang

et al. 2016

Fish & Shellfish Immunology

View full text Add to dashboard Cite

“…The ACP activities were evaluated following the protocol described by Zhao et al [43] using tissue homogenates instead of cells. The complement contents were assayed using a complement 3 (C3) or a complement 4 (C4) kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) following the manufacturer's protocols.…”

Section: Biochemical Analysismentioning

confidence: 99%

Dietary myo-inositol modulates immunity through antioxidant activity and the Nrf2 and E2F4/cyclin signalling factors in the head kidney and spleen following infection of juvenile fish with Aeromonas hydrophila

Jiang

Liu

et al. 2016

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Effects of potential probiotic Bacillus subtilis T13 on growth, immunity and disease resistance against Vibrio splendidus infection in juvenile sea cucumber Apostichopus japonicus

Cited by 174 publications

References 42 publications

The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes

The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes

Dietary low or excess levels of lipids reduced growth performance, and impaired immune function and structure of head kidney, spleen and skin in young grass carp (Ctenopharyngodon idella) under the infection of Aeromonas hydrophila

Dietary myo-inositol modulates immunity through antioxidant activity and the Nrf2 and E2F4/cyclin signalling factors in the head kidney and spleen following infection of juvenile fish with Aeromonas hydrophila

Contact Info

Product

Resources

About