Effects of probiotic administration on immune responses of children and adolescents with type 1 diabetes to a quadrivalent inactivated influenza vaccine

Bianchini, Sonia; Orabona, Ciriana; Camilloni, Barbara; Berioli, Maria Giulia; Argentiero, Alberto; Matino, Davide; Alunno, Anna; Albini, Elisa; Vacca, Carmine; Pallotta, Maria Teresa; Mancini, Giulia; Tascini, Giorgia; Toni, Giada; Mondanelli, Giada; Silvestri, Ettore; Grohmann, Ursula; Esposito, Susanna

doi:10.1080/21645515.2019.1633877

Cited by 27 publications

(44 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These patients represent a subcohort of a larger cohort recruited at the Pediatric Clinic of the Perugia University (Italy), as described elsewhere. 2 Patients affected by concomitant chronic diseases other than T1D, infections or subjected to immunosuppressive therapy were excluded from the study. Patients have been randomly assigned to two experimental groups on the basis of a computer-generated list.…”

Section: Materials and Methods Subjectsmentioning

confidence: 99%

“…Source conditions were optimized as follows: capillary temperature 250°C, heater temperature 270°C, S-lens RF level 50, sheath gas flow rate 40 and auxiliary gas flow rate 15 (arbitrary units). The acquisition was achieved in full MS/dd-MS 2 mode. The m/z range was from 100 to 900 with a resolving power equal to 70 000 FWHM ( m/z 200).…”

Section: Methodsmentioning

confidence: 99%

“…Mass accuracy tolerance was lower than 5 ppm. An inclusion list for dd-MS 2 experiments was used including the m/z precursor and the expected retention time (±1 minute) of each metabolite. The precursor ion was filtered by the quadrupole with a mass isolation window of ±0.9 Da.…”

Section: Methodsmentioning

confidence: 99%

“…Kyn was detected at 360 nm by an UV detector. For assessment of cytokine production, T1D PBMCs were activated in vitro with 1 μg/mL phytohemagglutinin (PHA; Sigma-Aldrich, MO, USA) for 48 hours, as described, 2 before harvesting culture supernatants. A 15-plex immunoassay (Bio-Plex Pro Human Th17 cytokine panel, Cat N. 171aa001m; Bio-Rad, CA, USA) and a MAGPIX system (Luminex Corporation) were used for the detection of IFN-γ, IL-17F, IL-31, IL-6, and sCD40L.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Effect of Probiotic Administration on Serum Tryptophan Metabolites in Pediatric Type 1 Diabetes Patients

Mondanelli

Orecchini

Volpi

et al. 2020

Int J�Tryptophan�Res

Self Cite

View full text Add to dashboard Cite

Type 1 diabetes (T1D) is characterized by anomalous functioning of the immuno regulatory, tryptophan-catabolic enzyme indoleamine 2,3 dioxygenase 1 (IDO1). In T1D, the levels of kynurenine—the first byproduct of tryptophan degradation via IDO1—are significantly lower than in nondiabetic controls, such that defective immune regulation by IDO1 has been recognized as potentially contributing to autoimmunity in T1D. Because tryptophan catabolism—and the production of immune regulatory catabolites—also occurs via the gut microbiota, we measured serum levels of tryptophan, and metabolites thereof, in pediatric, diabetic patients after a 3-month oral course of Lactobacillus rhamnosus GG. Daily administration of the probiotic significantly affected circulating levels of tryptophan as well as the qualitative pattern of metabolite formation in the diabetic patients, while it decreased inflammatory cytokine production by the patients. This study suggests for the first time that a probiotic treatment may affect systemic tryptophan metabolism and restrain proinflammatory profile in pediatric T1D.

show abstract

Section: Materials and Methods Subjectsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Probiotic Administration on Serum Tryptophan Metabolites in Pediatric Type 1 Diabetes Patients

Mondanelli

Orecchini

Volpi

et al. 2020

Int J�Tryptophan�Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…Bei einer weiteren Interventionsstudie wurde überprüft, ob es bei einer dreimonatigen Supplementation mit Lactobacillus rhamnosus GG bei an Typ-1-Diabetes Erkrankten zu einer verstärkten Immunfunktion infolge einer Influenzaimpfung kommt. Es konnte keine Verbesserung der humoralen Immunantwort festgestellt werden, allerdings wurde durch die Supplementation eine antiinflammatorische Wirkung induziert [134].…”

Section: Ddg-praxisempfehlungunclassified

Empfehlungen zur Ernährung von Personen mit Typ-1-Diabetes mellitus

Rubin

Bosy‐Westphal

Kabisch

et al. 2020

Diabetologie und Stoffwechsel

View full text Add to dashboard Cite

Probiotics, prebiotics, and synbiotics in type 1 diabetes mellitus: A systematic review and meta‐analysis of clinical trials

Moravejolahkami

Shakibaei

McGrattan

et al. 2023

Diabetes Metabolism Res

View full text Add to dashboard Cite

Dysbiosis or imbalance of microbes in the gut has been associated with susceptibility and progression of type 1 diabetes mellitus (T1DM). The present systematic review and meta‐analysis examined the effects of probiotics, prebiotics, and synbiotics on fasting blood glucose (FBG), haemoglobin A1c (HbA1c), C‐peptide, and insulin requirements in T1DM patients. A systematic search for trials published up to October 2022 was conducted in PubMed, EMBASE, Scopus, Google Scholar, ScienceDirect, Web of Science, and the Central Cochrane Library. Random effect models were used to synthesise quantitative data by STATA14. After the evaluation of 258 identified entries, five randomised controlled trials (n = 356; mean age = 11.7 years old) were included. The pooled effect size showed that FBG decreased following probiotic supplementation (weighted mean difference = −31.24 mg/dL; 95% confidence interval = −45.65, −16.83; p < 0.001), however, there was no significant improvement in serum HbA1c, C‐peptide, and insulin requirements. Probiotic supplementation could be a complementary therapeutic strategy in T1DM. The evidence is limited; therefore, it is crucial to conduct more trials.

show abstract

Effects of probiotic administration on immune responses of children and adolescents with type 1 diabetes to a quadrivalent inactivated influenza vaccine

Cited by 27 publications

References 37 publications

Effect of Probiotic Administration on Serum Tryptophan Metabolites in Pediatric Type 1 Diabetes Patients

Effect of Probiotic Administration on Serum Tryptophan Metabolites in Pediatric Type 1 Diabetes Patients

Empfehlungen zur Ernährung von Personen mit Typ-1-Diabetes mellitus

Probiotics, prebiotics, and synbiotics in type 1 diabetes mellitus: A systematic review and meta‐analysis of clinical trials

Contact Info

Product

Resources

About