Effects of Processing and Storage onPediococcus pentosaceusSB83 in Vaginal Formulations: Lyophilized Powder and Tablets

Abstract

Vaginal probiotics have an important role in preventing the colonization of the vagina by pathogens. This study aimed to investigate different formulations with Pediococcus pentosaceus SB83 (lyophilized powder and tablets with and without retarding polymer) in order to verify its stability and antilisterial activity after manufacture and during storage. The bacteriocinogenic activity of P. pentosaceus SB83 against Listeria monocytogenes was evaluated in simulated vaginal fluid. Suspension of Pediococcus pentosaceus SB83 reduced the pathogen only after 2 h and the lyophilized bacteria after 24 h of contact, and, in the tablets, P. pentosaceus SB83 lost the antimicrobial activity. The pH of simulated vaginal fluid decreased for all the tested conditions. As lyophilized powder demonstrated better results concerning antimicrobial activity, this formulation was selected to evaluate the antilisterial activity during the 12 months of storage. During storage at room temperature, lyophilized bacteria totally inhibited the pathogen only until one month of storage. At 4°C, P. pentosaceus SB83 showed antimicrobial activity during all the time of storage investigated. Therefore, the better formulation of P. pentosaceus SB83 is the lyophilized powder stored at 4°C, which may be administered intravaginally as a washing solution.

Probiotic lactobacilli in formulas and hygiene products for the health of the urogenital tract

“…The viable bacteria are incorporated frequently as powder, being lyophilization the gold standard technique for microbial preservation and stability during the storage for different time periods. [119][120][121][122] The selection of the appropriate dosage form depends on the physicochemical features of the delivered drug or formula, the target for them and women´s acceptance. 123…”

Studies On Species Of Lactic Acid Bacteria Isolate Sr 12 From Bali Cattle Gastric With Conventional Method And Api Kit 50ch

“…Pediococcus pentosaceus bacteria have various benefits, especially as probiotics. Borges et al in his study said Pediococcus pentosaceus in the form of frozen dry powder has the ability to inhibit pathogenic bacteria such as Listeria monocytogenes [8]. These bacteria can also be used as anti-obesity drugs by reducing the Body mass Index (BMI), waist circumference, and fat in the body…”

LAB bacteriocin applications in the last decade

“…Fermenticin HV6b has also been demonstrated to inhibit pathogenic vaginal bacteria such as Gardnerella vaginalis [24,50]. Other bacteriocins, such as SB83 produced by Pediococcus pentosaceus SB83 [51], enterocin 62-6, two peptides produced by E. faecium [52] and lactocin 160 (a peptide like-bacteriocin) produced by Lactobacillus rhamnosus [53] have exhibited effective action against G. vaginalis and Prevotella bivia, Bacteroides, Peptostreptococcus and Mobiluncus spp.…”