Effects of pulsed electromagnetic fields on histomorphometry and osteocalcin in disuse osteoporosis rats

Li, Baolin; Bi, Jianjun; Li, Wei; Huang, Sainan; Zhang, Shuangli; Zhao, Jiangang; Meng, Qinggang; Fei, Jianfeng

doi:10.3233/thc-171301

Cited by 14 publications

(12 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, this assumption requires further investigation. Examples are the studies of Cai et al [82], Li et al [83], or Jing et al [84], which investigated the effect of ELF-PEMF on architecture and mechanical properties of bone in diabetic animals, known to have skeletal deficiencies, or the studies of Lei et al [74,85], Li et al [86], Zhou et al [87,88], or Androjna et al [89], which investigated the effect of ELF-PEMF on bone quality in osteoporotic animals.…”

Section: Elf-pemf Effects On Bone Cell Functionmentioning

confidence: 99%

Translational Insights into Extremely Low Frequency Pulsed Electromagnetic Fields (ELF-PEMFs) for Bone Regeneration after Trauma and Orthopedic Surgery

Ehnert

Schröter

Aspera-Werz

et al. 2019

JCM

View full text Add to dashboard Cite

The finding that alterations in electrical potential play an important role in the mechanical stimulation of the bone provoked hype that noninvasive extremely low frequency pulsed electromagnetic fields (ELF-PEMF) can be used to support healing of bone and osteochondral defects. This resulted in the development of many ELF-PEMF devices for clinical use. Due to the resulting diversity of the ELF-PEMF characteristics regarding treatment regimen, and reported results, exposure to ELF-PEMFs is generally not among the guidelines to treat bone and osteochondral defects. Notwithstanding, here we show that there is strong evidence for ELF-PEMF treatment. We give a short, confined overview of in vitro studies investigating effects of ELF-PEMF treatment on bone cells, highlighting likely mechanisms. Subsequently, we summarize prospective and blinded studies, investigating the effect of ELF-PEMF treatment on acute bone fractures and bone fracture non-unions, osteotomies, spinal fusion, osteoporosis, and osteoarthritis. Although these studies favor the use of ELF-PEMF treatment, they likewise demonstrate the need for more defined and better controlled/monitored treatment modalities. However, to establish indication-oriented treatment regimen, profound knowledge of the underlying mechanisms in the sense of cellular pathways/events triggered is required, highlighting the need for more systematic studies to unravel optimal treatment conditions.

show abstract

Section: Elf-pemf Effects On Bone Cell Functionmentioning

confidence: 99%

Translational Insights into Extremely Low Frequency Pulsed Electromagnetic Fields (ELF-PEMFs) for Bone Regeneration after Trauma and Orthopedic Surgery

Ehnert

Schröter

Aspera-Werz

et al. 2019

JCM

View full text Add to dashboard Cite

show abstract

“…Electromagnetic fields (EMFs) of extremely low frequency have been considered to be a promising therapy for a wide range of bone diseases, such as fresh and nonunion fractures, osteoarthritis, and osteoporosis (Funk, Monsees, & Özkucur, ). Accumulating evidence has shown that EMFs promote osteogenic differentiation and mineralization of bone marrow stromal cells (BMSCs) and osteoblasts, inhibit osteoclast formation and its bone resorption activity, and prevent bone loss in animal models of disuse and ovariectomy‐induced osteoporosis (Barnaba et al, ; Chang, Chang, Tsai, & Shih, ; Chang, Chang, Wu, & Shih, ; Jing et al, , ; He, Selvamurugan, Warshaw, & Partridge, ; Hong, Kang, Yi, Kim, & Cho, ; Li et al, ; Ongaro et al, ; Schwartz et al, ; Tong et al, ; Xie et al, ; Yan et al, ; J. Zhang et al, ; Zhou et al, ). Clinical investigations further confirmed that EMFs increased bone mineral density (BMD) and prevented bone loss in the patients with osteoporosis (Garland, Adkins, Matsuno, & Stewart, ; Tabrah, Ross, Hoffmeier, & Gilbert, ).…”

Section: Introductionmentioning

confidence: 99%

Pulsed electromagnetic fields promote bone formation by activating the sAC–cAMP–PKA–CREB signaling pathway

Wang

Shi

et al. 2018

Journal Cellular Physiology

View full text Add to dashboard Cite

The application of pulsed electromagnetic fields (PEMFs) in the prevention and treatment of osteoporosis has long been an area of interest. However, the clinical application of PEMFs remains limited because of the poor understanding of the PEMF action mechanism. Here, we report that PEMFs promote bone formation by activating soluble adenylyl cyclase (sAC), cyclic adenosine monophosphate (cAMP), protein kinase A (PKA), and cAMP response element-binding protein (CREB) signaling pathways. First, it was found that 50 Hz 0.6 millitesla (mT) PEMFs promoted osteogenic differentiation of rat calvarial osteoblasts (ROBs), and that PEMFs activated cAMP-PKA-CREB signaling by increasing intracellular cAMP levels, facilitating phosphorylation of PKA and CREB, and inducing nuclear translocation of phosphorylated (p)-CREB. Blocking the signaling by adenylate cyclase (AC) and PKA inhibitors both abolished the osteogenic effect of PEMFs. Second, expression of sAC isoform was found to be increased significantly by PEMF treatment. Blocking sAC using sAC-specific inhibitor KH7 dramatically inhibited the osteogenic differentiation of ROBs. Finally, the peak bone mass of growing rats was significantly increased after 2 months of PEMF treatment with 90 min/day. The serum cAMP content, p-PKA, and p-CREB as well as the sAC protein expression levels were all increased significantly in femurs of treated rats. The current study indicated that PEMFs promote bone formation in vitro and in vivo by activating sAC-cAMP-PKA-CREB signaling pathway of osteoblasts directly or indirectly.

show abstract

“…The intensity of PEMF used in 20 studies ranged from 0.1 to 3.82 mT, with three studies using the intensity of 80 µA/cm 2 , 30 mW/cm 2 , and 1-2 mV/cm, respectively [Chang and Chang, 2003;Lirani-Galvão et al, 2006;Behari, 2008], and PEMF intensity used by one article was 1 T [Lin et al, 2020]. Eighteen articles provided details of the time of PEMF initiation, varying from the day after OVX to 12 weeks after the operation; six articles did not provide any information on the time of PEMF initiation [Jing et al, 2013;Zhou et al, 2013;Li et al, 2017aLi et al, , 2018Lei et al, 2018].…”

Section: Characteristics Of Animals and Groupsmentioning

confidence: 99%

Efficacy of Pulsed Electromagnetic Fields on Experimental Osteopenia in Rodents: A Systematic Review

Wang

Xie

Zhu

et al. 2021

Bioelectromagnetics

View full text Add to dashboard Cite

Osteoporosis leads to increased bone fragility and risk of fractures. Different strategies have been employed to reduce bone loss, including the use of a pulsed electromagnetic field (PEMF). Although many experimental studies have demonstrated the effect of PEMF on reduction of bone loss, the outcomes studied are varied and insufficient, and the quality of evidence is unknown. Therefore, the aim of this review was to assess the preclinical evidence on the effect of PEMF on bone loss. The existing challenges were also evaluated, and suggestions were provided to strengthen the quality of evidence in future studies. All original articles concerning the effect of PEMF on osteoporosis in animal models were included. Twenty-four studies met the inclusion criteria, 23 of which suggested that PEMF was effective in reducing bone loss, while one study failed to demonstrate any benefit. Risk of bias analysis suggested that information on key measures to reduce bias was frequently not reported. Animal models for osteoporosis, PEMF intervention regimens, outcomes, and specific bone detection sites seemed to influence the efficacy of PEMF in osteoporosis. Our results indicate the potential benefits of PEMF selection in animal models of osteoporosis. However, due to the heterogeneity of the parameters and the quality of the included literature, comprehensive studies using standardized protocols are warranted to confirm the results.

show abstract

Effects of pulsed electromagnetic fields on histomorphometry and osteocalcin in disuse osteoporosis rats

Cited by 14 publications

References 15 publications

Translational Insights into Extremely Low Frequency Pulsed Electromagnetic Fields (ELF-PEMFs) for Bone Regeneration after Trauma and Orthopedic Surgery

Translational Insights into Extremely Low Frequency Pulsed Electromagnetic Fields (ELF-PEMFs) for Bone Regeneration after Trauma and Orthopedic Surgery

Pulsed electromagnetic fields promote bone formation by activating the sAC–cAMP–PKA–CREB signaling pathway

Efficacy of Pulsed Electromagnetic Fields on Experimental Osteopenia in Rodents: A Systematic Review

Contact Info

Product

Resources

About