Effects of salpingitis simulation on the morphology and expression of inflammatory-related genes of oviduct in laying hens

Li, L.L.; Xu, P.T.; Liu, Z.P.; Liu, C.A.; Dong, Xin; Zhang, Z.F.; Guo, Shuangshuang; Ding, Biao

doi:10.1016/j.psj.2022.102246

Cited by 4 publications

(3 citation statements)

References 23 publications

(23 reference statements)

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“…Although Salmonella contamination does not alter egg content, color, smell, and consistency, vigilance for human health remains crucial. Meanwhile, Ahmed et al (2010) and Li et al (2023) reported that the effect of the ovary and oviduct dysfunction disorders if exposed to SE for a long is oophoritis and salpingitis. In laying hens, salpingitis is characterized by a decrease in egg production, no or short peak laying time, poor quality of egg shells, and increased production of thin, soft, and sandy eggshells.…”

Section: Resultsmentioning

confidence: 99%

Microbial Count and AvBD10 Expressions in Ovaries and Oviducts of Kampung Unggul Balitbangtan (KUB)-1 Chickens Following Intravaginally CpG-ODN and S. Enteritidis

Suryohastari,

Sumarsono,

Giri-rachman

et al. 2024

J.Tropical Biodiversity Biotechnology

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Indonesia boasts diverse native chickens (Gallus gallus domesticus) known for more disease resistance in comparison to broiler chicken, and Kampung Unggul Balitbangtan (KUB)-1 is designated as Indonesia's superior breed. Salmonella Enteritidis (SE) is associated with salmonellosis, a foodborne illness that can be transmitted by transovarial, wherein colonisation in the oviduct ascends to the ovaries. However, studies mimicking transovarial salmonellosis via intravaginal treatment of chicken have been limited. Meanwhile, Cytosine-phosphate-guanine oligodeoxynucleotide (CpG-ODN) stimulation has been known to induce avian β-defensins (AvBDs). This in vivo study aimed to determine the effects of intravaginal CpG-ODN treatment and SE challenged on microbial count and AvBD10 expression regarding the potential of intravaginally CpG-ODN to enhance innate immunity as an alternative approach against transovarial Salmonellosis. A total of 39 KUB-1 chickens were divided into four groups: T1 (CpG-ODN treatment), T2 (SE treatment), T3 (CpG-ODN treatment + challenged with SE), and C (Control). Observation was carried out from day 1 to day 4 post-intravaginal (PI). We found a significant increase in ovarian microbial count (p≤0.05). Notably, ovaries and oviducts remained uncontaminated post-SE challenge. Intravaginal CpG-ODN treatment significantly upregulated AvBD10 in both ovaries (p=0.016) and oviducts (p=0.023). Therefore, KUB-1 chickens exhibit SE immunity, and intravaginal CpG-ODN administration holds promise for preventing transovarial Salmonellosis in laying hens.

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Section: Resultsmentioning

confidence: 99%

Microbial Count and AvBD10 Expressions in Ovaries and Oviducts of Kampung Unggul Balitbangtan (KUB)-1 Chickens Following Intravaginally CpG-ODN and S. Enteritidis

Suryohastari,

Sumarsono,

Giri-rachman

et al. 2024

J.Tropical Biodiversity Biotechnology

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“…A large number of CaCO 3 synthetic precursors were transported to uterine fluid through uterine gland epithelial cells to promote eggshell formation ( Li et al, 2023 ). The transcriptome analysis showed that high and low hatchability group differences of uterus in ion transport capacity.…”

Section: Discussionmentioning

confidence: 99%

“…The CO 2 in the blood is the main substance in the formation of bicarbonate, and it is catalyzed to HCO 3 − after simple diffusion through the plasma membrane into the glandular cells of the uterus ( Li et al, 2023 ). Due to the outflow of ions, additional ion transfer is required to maintain cell homeostasis.…”

Section: Discussionmentioning

confidence: 99%

High albumen height by expression of GALNT9 and thin eggshell by decreased Ca2+ transportation caused high hatchability in Huainan partridge chicken

Yang,

Zhao,

Dou

et al. 2024

Poultry Science

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Organic zinc and manganese enhance eggshell ultrastructure by influencing its calcium deposition to improve eggshell quality in laying hens

Li,

Duan,

Chen

et al. 2024

J Sci Food Agric

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BackgroundThis study aimed to explore the mechanism of organic zinc and manganese (OZM) influencing eggshell quality of laying hens. A total of 384 21‐week‐old Jingfen‐1 laying hens were randomly divided into four groups (8 replicates per group, 12 hens per replicate): control group (60 mg kg−1 Zn as zinc sulfate and 80 mg kg−1 Mn as manganese sulfate) and OZM groups (5, 10 and 15 mg kg−1 Zn as yeast zinc and 80 mg kg−1 Mn as manganese methionine).ResultsCompared with the control group, OZM_5 and OZM_10 groups significantly increased feed intake and egg weight in laying hens in 28–31 weeks (P < 0.05) while egg weight and shell weight were increased in all the OZM groups (P < 0.05). In addition, the OZM_15 group significantly decreased the mammillary knob width and mammillary layer thickness and increased the palisade layer thickness and total effective thickness (P < 0.05), and had a stronger and more stable Ca signal strength in the mammillary layer and a more uniform and dense distribution of calcium and zinc in the transversal surfaces of eggshell. Furthermore, mRNA levels of Claudin2, Zona Occludens 1, Alkaline phosphatase and Ca2+ATPase were significantly upregulated in the OZM_15 group of laying hens at week 31 (P < 0.05).ConclusionDietary supplementation of 10–15 mg kg−1 yeast zinc and 80 mg kg−1 manganese methionine can enhance eggshell ultrastructure by influencing its endometrial barrier function and calcium deposition, thereby improving eggshell quality. © 2024 Society of Chemical Industry.

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Effects of salpingitis simulation on the morphology and expression of inflammatory-related genes of oviduct in laying hens

Cited by 4 publications

References 23 publications

Microbial Count and <i>AvBD10</i> Expressions in Ovaries and Oviducts of Kampung Unggul Balitbangtan (KUB)-1 Chickens Following Intravaginally CpG-ODN and <i>S</i>. Enteritidis

Microbial Count and <i>AvBD10</i> Expressions in Ovaries and Oviducts of Kampung Unggul Balitbangtan (KUB)-1 Chickens Following Intravaginally CpG-ODN and <i>S</i>. Enteritidis

High albumen height by expression of GALNT9 and thin eggshell by decreased Ca2+ transportation caused high hatchability in Huainan partridge chicken

Organic zinc and manganese enhance eggshell ultrastructure by influencing its calcium deposition to improve eggshell quality in laying hens

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