Effects of Selenium, Copper and Magnesium on Antioxidant Enzymes and Lipid Peroxidation in Bovine Fluorosis

Abstract

The antioxidant enzymes, lipid peroxidation and free radicals assessment were made of the effects of selenium, copper and magnesium on bovine endemic fluorosis under high fluoride, low selenium and low copper productive conditions. Thirty-two beef cattle were selected from high fluoride area, and randomly divided into four groups with eight cattle each as follows: (1) high fluoride control group (HFC); (2) supplemented group with 0.25 mg/kg selenium (HFSe); (3) supplemented group with 15 mg/kg copper (HFCu) and (4) supplemented group with 0.25 mg/kg selenium+15 mg/kg copper+1 mg/kg magnesium (HFSeCuMg) per day for 83 days. Moreover, eight beef cattle were selected from non-high fluoride area as normal control group. Blood samples were collected from cattle on 0 d, 30 d and 83 d respectively, to analyze the enzyme activities and concentration of GSH-px, CAT, SOD, MDA and free radicals. The results showed that the contents of free radicals and MDA in HFC group were significantly higher, and the whole blood GSH-px, CAT, erythrocyte SOD activities were lower than the normal control group. Free radicals, metabolic imbalance and antioxidant disorder therefore, play an important role in fluorosis. However, GSH-px, CAT and SOD activities in HFSe group and HFSeCuMg group at 30 d and 83 d were markedly higher than the same groups at the 0 d and the HFC group at the same time. Likewise, there was a corresponding reduction in the contents of free radicals and MDA. These findings indicated that supplementation with selenium, copper and magnesium elevated high fluoride bovine antioxidant enzymes, and decreased MDA and free radicals contents. But, the activities of supplementation selenium group did not increase until day 83. These results demonstrated that fluorosis was associated with lower serum Se and Cu levels than in the control, and it was therefore concluded that fluorosis is associated with decreased serum levels of these minerals. Long-term high fluoride intake under productive condition enhances oxidative stress in the blood, thereby disturbing the antioxidant defense of cattle. Increased oxidative stress could be one of the mediating factors in the pathogenesis of toxic manifestations of fluoride. It is benefical for high fluoride cattle supplemented with proper selenium, copper and magnesium to increase fluoride excretion and obtain the protective impact of the activity of oxidative enzymes, and to decrease lipid peroxidation and free radicals contents.

Effects of alpha‐lipoic acid supplementation on antioxidative ability and performance of sows and nursing piglets

“…In the present study, LA supplementation resulted in an increase in serum GSH‐Px and SOD activity, in accordance with the decrease in MDA concentration, consisting with the results reported in other animals. Maleic dialdehyde is a main marker of endogenous lipid peroxidation and the activity levels of the antioxidant enzymes GSH‐Px, CuZnSOD and CAT (Han et al., 2004). In the current study, increasing maternal dietary LA levels decreased the serum MDA levels in both of lactation sows and piglets, demonstrating that maternal supplementation of LA increases antioxidant capacity in both sows and their piglets by the increase in activities of the antioxidant enzymes.…”

Effects of pre‐slaughter stressor and feeding preventative Chinese medicinal herbs on glycolysis and oxidative stability in pigs

“…MDA is a primary marker of endogenous lipid peroxidation, and the activity levels of the antioxidant enzymes GSH‐Px, CuZnSOD and CAT represent the anti‐oxidative capacity of animals (Han et al . ). Therefore, dietary supplementation with LB could be beneficial to the stressed pig with a reduced risk of PSE, subsequently improving the profit of the swine industry.…”

“…Polysaccharides isolated from LB significantly decreases oxidative stress through attenuating lipid peroxidation and enhancing antioxidant enzymes activities (Wang et al 2002;Li et al 2007;Niu et al 2008). MDA is a primary marker of endogenous lipid peroxidation, and the activity levels of the antioxidant enzymes GSH-Px, CuZnSOD and CAT represent the anti-oxidative capacity of animals (Han et al 2004). Therefore, dietary supplementation with LB could be beneficial to the stressed pig with a reduced risk of PSE, subsequently improving the profit of the swine industry.…”

Evaluation of glutathione s‐transferase as toxicity indicator for roxarsone and arsanilic acid in Eisenia fetida

“…Our results clearly show increased values of the GST enzyme activity in response to the higher concentrations of roxarsone and arsanilic acid in the early days of treatment. Similarly, oxidative stress has been depicted by a number of researchers after treatment with arsenic-releasing compound and can result in different kinds of organ injuries of the organisms (Czaja, 2002;Tuder et al, 2003;Han et al, 2004Han et al, , 2006. Exposure to stressors can up-or down-regulate various genes that have protective functions.…”