Effects of Selenium on MAC-T Cells in Bovine Mastitis: Transcriptome Analysis of Exosomal mRNA Interactions

Jing, Hongyuan; Chen, Yu; Liang, Wan; Chen, Miaoyu; Qiu, Changwei; Guo, Mengyao

doi:10.1007/s12011-020-02439-7

Cited by 10 publications

(16 citation statements)

References 44 publications

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“…In vitro studies based on bovine neutrophils showed that Se supplementation enhances chemotactic migration, phagocytosis and serum superoxide dismutase (SOD) activity [ 41 ], and intracellular antibacterial activity against S. aureus [ 42 ]. More recent studies have suggested [ 43 ] that selenium may play a crucial role in immune and inflammatory regulation by influencing the differential expression of exosomal mRNAs of key genes in bovine mastitis. They demonstrated that selenium affects mRNA expression in MAC-T-cell-derived exosomes related to immunity and inflammatory pathways.…”

Section: Seleniummentioning

confidence: 99%

“…Selenium significantly inhibits the expression of NLRP3, ASC, Caspase-1, Caspase-1 p20 and Pro-IL-1β. Dietary selenium can attenuate Staphylococcus aureus mastitis by the inhibition of the NLRP3 inflammasome [ 43 ]. In addition, a beneficial effect on mastitis treatment was observed when affected cows were injected with a multimineral preparation containing Se [ 41 , 51 ].…”

Section: Seleniummentioning

confidence: 99%

See 1 more Smart Citation

The Association between Selected Dietary Minerals and Mastitis in Dairy Cows—A Review

Libera

Konieczny

Witkowska

et al. 2021

Animals

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The aim of this paper is to describe the association between selected dietary minerals and mastitis in dairy cows. Minerals are a group of nutrients with a proven effect on production and reproductive performance. They also strongly affect immune system function. In particular their deficiencies may result in immunosuppression, which is a predisposing factor for udder inflammation occurrence. The role of selected dietary minerals (including calcium, phosphorus, magnesium, selenium, copper and zinc) has been reviewed. Generally, minerals form structural parts of the body; as cofactors of various enzymes they are involved in nerve signaling, muscle contraction and proper keratosis. Their deficiencies lead to reduced activity of immune cells or malfunction of teat innate defense mechanisms, which in turn promote the development of mastitis. Special attention was also paid to minerals applied as nanoparticles, which in the future may turn out to be an effective tool against animal diseases, including mastitis. To conclude, minerals are an important group of nutrients, which should be taken into account on dairy farms when aiming to achieve high udder health status.

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Section: Seleniummentioning

confidence: 99%

Section: Seleniummentioning

confidence: 99%

The Association between Selected Dietary Minerals and Mastitis in Dairy Cows—A Review

Libera

Konieczny

Witkowska

et al. 2021

Animals

View full text Add to dashboard Cite

show abstract

“…68 Exosomal selenoprotein mRNAs,tRNA Sec enter cancer cells and may be involved in regulating selenoprotein synthesis. 67,69 Proteins in which Se is incorporated nonspecifically, Sec and selenomethionine, can replace their respective sulfur counterparts, cysteine, and methionine. The physiological consequences of this nonspecific incorporation remain unclear.…”

Section: Selenoenzyme (Trxr1 or Gpx4)-specific Target Biomarkersmentioning

confidence: 99%

“…Diverse microRNA and mRNA transfer signals between tumor cells and the TME via direct contact with different types of extracellular vesicles. Exosomes have been discovered as the key factors in facilitating cell‐to‐cell communication between tumor cells and the microenvironment, which takes part in signaling pathways in the TME (Figure 2), and have the potential capabilities to modulate the TME and contribute to tumor metastasis, immune response, 66,67 and multidrug resistance 68 . Exosomal selenoprotein mRNAs,tRNA Sec enter cancer cells and may be involved in regulating selenoprotein synthesis 67,69 …”

Section: Selenoenzyme (Trxr1 or Gpx4)‐specific Target Biomarkersmentioning

confidence: 99%

The emerging role of selenium metabolic pathways in cancer: New therapeutic targets for cancer

Kalishwaralal

Keerthana

Mohan

et al. 2021

J of Cellular Biochemistry

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Selenium (Se) is incorporated into the body via the selenocysteine (Sec) biosynthesis pathway, which is critical in the synthesis of selenoproteins, such as glutathione peroxidases and thioredoxin reductases. Selenoproteins, which play a key role in several biological processes, including ferroptosis, drug resistance, endoplasmic reticulum stress, and epigenetic processes, are guided by Se uptake. In this review, we critically analyze the molecular mechanisms of Se metabolism and its potential as a therapeutic target for cancer. Sec insertion sequence binding protein 2 (SECISBP2), which is a positive regulator for the expression of selenoproteins, would be a novel prognostic predictor and an alternate target for cancer. We highlight strategies that attempt to develop a novel Se metabolism-based approach to uncover a new metabolic drug target for cancer therapy. Moreover, we expect extensive clinical use of SECISBP2 as a specific biomarker in cancer therapy in the near future. Of note, scientists face additional challenges in conducting successful research, including investigations on anticancer peptides to target SECISBP2 intracellular protein.

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“…Several studies have used MAC-T cells to describe LPSinduced mastitis and in therapeutic studies for mastitis (Geng et al, 2020;Jing et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

High-dose lipopolysaccharide induced autophagic cell death in bovine mammary alveolar cells

Park¹,

Yeo²,

Cho³

et al. 2022

J Anim Reprod Biotechnol

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Bovine mammary epithelial (MAC-T) cells are commonly used to study mammary gland development and mastitis. Lipopolysaccharide is a major bacterial cell membrane component that can induce inflammation. Autophagy is an important regulatory mechanism participating in the elimination of invading pathogens. In this study, we evaluated the mechanism underlying bacterial mastitis and mammary cell death following lipopolysaccharide treatment. After 24 h of 50 μg/mL lipopolysaccharide treatment, a significant decrease in the proliferation rate of MAC-T cells was observed. However, no changes were observed upon treatment of MAC-T cells with 10 μg/mL of lipopolysaccharide for up to 48 h. Thus, upon lipopolysaccharide treatment, MAC-T cells exhibit dose-dependent effects of growth inhibition at 10 μg/mL and death at 50 μg/mL. Treatment of MAC-T cells with 50 μg/mL lipopolysaccharide also induced the expression of autophagy-related genes ATG3, ATG5, ATG10, ATG12, MAP1LC3B, GABARAP-L2, and BECN1. The autophagy-related LC3A/B protein was also expressed in a dose-dependent manner upon lipopolysaccharide treatment. Based on these results, we suggest that a high dose of bacterial infection induces mammary epithelial cell death related to autophagy signals.

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Effects of Selenium on MAC-T Cells in Bovine Mastitis: Transcriptome Analysis of Exosomal mRNA Interactions

Cited by 10 publications

References 44 publications

The Association between Selected Dietary Minerals and Mastitis in Dairy Cows—A Review

The Association between Selected Dietary Minerals and Mastitis in Dairy Cows—A Review

The emerging role of selenium metabolic pathways in cancer: New therapeutic targets for cancer

High-dose lipopolysaccharide induced autophagic cell death in bovine mammary alveolar cells

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