Effects of simulated acid rain on pollen physiology and ultrastructure in the apple

Bellani, Lorenza; Rinallo, C.; Muccifora, Simonetta; Gori, P.

doi:10.1016/s0269-7491(96)00127-3

Cited by 13 publications

(6 citation statements)

References 25 publications

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“…Acid rain induces changes in the cellular biochemistry, physiology and ultrastructure of plants. For example, acid rain lead to decrease in photosynthetic rate, loss of nutrient from leaves, alterations of water balance and enzyme activities, and changes of pollen physiology and ultrastructure of chloroplasts and mitochondria (Bellani et al 1997;Gabara et al 2003;Van Huylenbroeck et al 2000). In this work, SiAR stress-induced changes in the proteome of P. massoniana needles were analyzed after treatment with SiAR for 8 months.…”

Section: Discussionmentioning

confidence: 99%

“…Acid rain also induces changes in the cellular biochemistry and physiology of the whole plant. Biological effects of acid rain on plants are numerous and complex, and include visible symptoms of injury (chlorosis and necrosis) and invisible effects such as reduced photosynthesis, nutrient loss from leaves, altered water balance, variation of several enzyme activities, changes of pollen physiology and ultrastructure (Bellani et al 1997;Van Huylenbroeck et al 2000). It was reported that the loss of forest ecological benefits caused by acid rain exceeded 16 billion Yuan (2.4 billion dollars) per year only in 11 provinces of south China (Feng 2000).…”

Section: Introductionmentioning

confidence: 99%

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Long-term effects of simulated acid rain stress on a staple forest plant, Pinus massoniana Lamb: a proteomic analysis

Wang

Liu

Niu

et al. 2012

Trees

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At present, acid rain has become one of the top ten global environmental issues. Acid rain causes slower growth, injury, or decline of forests. Some dramatic effects on forests have been observed in south China since the late 1970s and the situation is deteriorating. We carried out a comparative proteomic analysis on Pinus massoniana Lamb, a staple tree species widely distributed in middle and south China to gain a better understanding of tree response to acid rain at molecular level. Two-year-old P. massoniana saplings were treated with simulated AR (SiAR) or control solution, respectively, for 8 months. The changes in total protein profile of P. massoniana leaves were studied using two-dimensional differential gel electrophoresis (2D-DIGE). Among the total protein spots reproducibly detected on each gel, 65 spots representing 28 proteins were identified to be differentially regulated. These proteins were annotated in various biological functions, such as photosynthesis and energy metabolism, secondary metabolism, protein stability, amino acid and nitrogen metabolism and defense. Down-regulation of four key enzymes in the Calvin cycle identified that biomass loss by SiAR was mainly due to the inhibition of carbon fixation. Primary energy metabolisms involved in sucrose biosynthesis, glycolytic pathway and Krebs cycle, etc., were also disturbed after SiAR treatment. Specifically, most of up-regulated proteins were related to secondary metabolism, protein stability and defense, suggesting that in response to SiAR stress, plants started a variety of metabolic pathways to prevent cells from damage. Different from the herbaceous plants suffering SiAR, it revealed that secondary metabolites in P. massoniana play pivotal roles against SiAR. Protemoic techniques were demonstrated a reliable and robust tool to expand our understanding of differentially expressed proteins associated with acid rain stress on P. massoniana. Functional analysis of these proteins further revealed biochemical and physiological basis of the plant in response to acid rain and would provide strategies for breeding new acid rain tolerant tree species. To our knowledge, it is the first proteome report on the forest plant suffering long-term acid rain stress.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Long-term effects of simulated acid rain stress on a staple forest plant, Pinus massoniana Lamb: a proteomic analysis

Wang

Liu

Niu

et al. 2012

Trees

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“…Transects were also used, for instance, along a specific road [ 43 ] or according to the altitude [ 44 , 45 ]. In different continents, some authors investigated forest zones [ 46 – 51 ], sometimes under specific sophisticated ecological protocols [ 52 ] including the simulation of acid rain on selected trees from experimental orchards before the collect phase of pollen material [ 53 ]. Among other forest studies, a comparison of polluted versus nonpolluted populations was proposed.…”

Section: Effects Of Pollutants On Pollen Grains and Pollen Contentmentioning

confidence: 99%

A Review of the Effects of Major Atmospheric Pollutants on Pollen Grains, Pollen Content, and Allergenicity

Sénéchal

Visez

Charpin

et al. 2015

The Scientific World Journal

171

111

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This review summarizes the available data related to the effects of air pollution on pollen grains from different plant species. Several studies carried out either on in situ harvested pollen or on pollen exposed in different places more or less polluted are presented and discussed. The different experimental procedures used to monitor the impact of pollution on pollen grains and on various produced external or internal subparticles are listed. Physicochemical and biological effects of artificial pollution (gaseous and particulate) on pollen from different plants, in different laboratory conditions, are considered. The effects of polluted pollen grains, subparticles, and derived aeroallergens in animal models, in in vitro cell culture, on healthy human and allergic patients are described. Combined effects of atmospheric pollutants and pollen grains-derived biological material on allergic population are specifically discussed. Within the notion of “polluen,” some methodological biases are underlined and research tracks in this field are proposed.

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“…While declines in biomass are ascribed to a decrease in photosynthetic capacity [8], the interference with reproduction is likely caused by suppression of growth of certain reproductive structures [3]. For instance, pollen viability and germination have been found to decline with a decrease in pH of simulated acid rain in apple [9] and litchi [10].…”

Section: Introductionmentioning

confidence: 99%

Growth and Leaf Chlorophyll Content of Eucalyptus grandis W. Hill ex Maiden are Adversely Affected by Simulated Acid Rain

Buma

Ambebe

Fogwe

2020

JALSI

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The study assessed the effects of different pH values of simulated acid rain on growth and leaf chlorophyll content of Eucalyptus grandis. The treatments comprised of two simulated acid rain solutions of pH 4.0 and 3.5, and unacidified water at pH 6.5. Place and Duration of Study was the National Forestry Development Agency, Humid Savannah Zone, Bamenda I Sub-Division, North West Region, Cameroon, between June and September 2019, respectively. The experiment was conducted under field conditions. Thirty 3-month-old seedlings were exposed to each pH level at 7-day intervals using a spray bottle. While two sets of thirty seedlings were administered sulphuric acid calibrated tap water with pH values of 4.0 and 3.5, the third set that constituted the control was sprayed with normal tap water. Data were collected on morphology, biomass, and leaf chlorophyll content at the end of the study and subjected to analysis of variance and Scheffé’s test. Results: The pH 3.5 treatment resulted in significantly lower responses of height (12.95 cm), number of leaves (15.28), leaf area (23.96 cm2) and total biomass (0.61 g) than the other two pH levels that did not differ for any of the traits. Average values between pH 4.0 and control were 20.39 cm, 22.00, 45.66 cm2 and 1.77 g. Stem diameter and root collar diameter declined from 2.87 mm and 4.14 mm at the control to 2.54 mm and 3.78 mm at pH 3.5, respectively. Leaves at pH 3.5 showed signs of necrosis, drying and curling. Leaf chlorophyll content was significantly greater in the control (45.30 SPAD units) than in the pH 4.0 (40.01 SPAD units) and pH 3.5 (39.82 SPAD units) treatments that displayed similar responses. Conclusion: The study reveals that simulated acid rain at pH 3.5 can have a harmful effect on chlorophyll content and growth of Eucalyptus grandis.

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Effects of simulated acid rain on pollen physiology and ultrastructure in the apple

Cited by 13 publications

References 25 publications

Long-term effects of simulated acid rain stress on a staple forest plant, Pinus massoniana Lamb: a proteomic analysis

Long-term effects of simulated acid rain stress on a staple forest plant, Pinus massoniana Lamb: a proteomic analysis

A Review of the Effects of Major Atmospheric Pollutants on Pollen Grains, Pollen Content, and Allergenicity

Growth and Leaf Chlorophyll Content of Eucalyptus grandis W. Hill ex Maiden are Adversely Affected by Simulated Acid Rain

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