Effects of single nucleotide polymorphisms in the 5′-flanking region of heat shock protein 70.2 gene on semen quality in boars

Huang, San-Yuan; Chen, M.Y.; Lin, Eric S.; Tsou, H. L.; Kuo, Y. H.; Ju, C. C.; Lee, W.C.

doi:10.1016/s0378-4320(01)00202-0

Cited by 51 publications

(28 citation statements)

References 28 publications

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“…For example, Zhang et al (2002) detected polymorphisms in regulatory and coding regions of the Hsp70 gene associated with different heat tolerance capability in broiler chickens. Huang et al (2002) detected SNPs in the 5′ flanking region of the Hsp70.2 gene in boars. These SNPs have been associated with the quality of semen production during the summer.…”

Section: Discussionmentioning

confidence: 96%

Cellular thermotolerance is associated with heat shock protein 70.1 genetic polymorphisms in Holstein lactating cows

Basiricò

Morera

Primi

et al. 2011

Cell Stress and Chaperones

127

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Heat shock proteins (Hsp) are known to protect cells from several stressors. Nucleotide changes in the flanking regions [5′-and 3′-untranslated region (UTR)] of Hsp gene might affect inducibility, degree of expression, or stability of Hsp70 mRNA. The present study aimed to investigate the association between inducible Hsp70.1 single nucleotide polymorphisms (SNPs) and heat shock (HS) response of peripheral blood mononuclear cells (PBMC) in dairy cows. Four hundred forty-six Italian Holstein cows were genotyped for four Hsp70

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Section: Discussionmentioning

confidence: 96%

Cellular thermotolerance is associated with heat shock protein 70.1 genetic polymorphisms in Holstein lactating cows

Basiricò

Morera

Primi

et al. 2011

Cell Stress and Chaperones

127

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“…There was an effect of time for TACD and TNB (P < 0.01) and a tendency for FR (P ¼ 0.10). Pruneda et al 2005] or the quantification of only the PCD [Malmgren and Larsson 1984;Borg et al 1993;Andersson et al 1998;Huang et al 2002]. Within the present study, the means for the PCD (6.19 AE 0.76%), DCD (4.60 AE 0.38%), DMR (2.69 AE 0.48%) and TACD (13.49 AE 0.96%; data not shown) were similar to the results of some studies [Waberski et al 1994;Huang et al 2000;Gadea et al 2004;Sancho et al 2004] but not others, that showed higher levels of attached CDs [Petrunkina et al 2001;Kuster et al 2004b].…”

Section: Discussionmentioning

confidence: 99%

High Resolution Light Microscopic Evaluation of Boar Semen Quality Sperm Cytoplasmic Droplet Retention in Relationship with Boar Fertility Parameters

Lovercamp

Safranski

Fischer

et al. 2007

Archives of Andrology

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The purpose of this study was to investigate the relationship between fertility and quantitative measures of boar semen quality, including various patterns of sperm cytoplasmic droplet (CD) retention, as determined by high power differential interference contrast (DIC) microscopy. A total of 116 ejaculates were collected from a nucleus herd of 18 Large White boars over an eight month period. Semen quality parameters were analyzed for each ejaculate by calculating the percentage of normal spermatozoa, spermatozoa possessing a CD in the proximal, distal, or distal midpiece reflex position, total spermatozoa with an attached cytoplasmic droplet, spermatozoa with non-CD related aberrations and total spermatozoa with abnormalities. Of the 116 ejaculates received, 71 ejaculates from 13 boars had corresponding fertility data from single-sire inseminations of multiparous sows. The fertility data included farrowing rate (FR) and total number born (TNB). The monthly FR encompassed one month before and one month after the date of semen collection. Detection of differences for fertility and semen quality parameters was performed by separating the boars into either an above-average or below-average group based on the mean FR (74.01 AE 1.43%) or TNB (12.34 AE 0.17) for the study. For FR, the boars in the below-average group had a significantly lower percentage of normal spermatozoa and significantly higher percentage of spermatozoa possessing distal CDs, total attached CDs and total abnormalities compared to the boars in the above-average group. Conversely, for TNB there were no significant differences between the above-and below-average groups for the semen quality parameters. These data suggest that the attached CD may negatively affect FR, but not TNB. The detection of relationships between the boar fertility parameters and the retention of the sperm CD after ejaculation, document the advantage of high power DIC microscopy in conventional semen evaluation.

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“…Zhang et al (2002) detected, by PCR-SSCP and sequencing, polymorphism in regulatory and coding regions of the hsp70 gene in chickens with different heat tolerance capability. Huang et al (2002) detected single nucleotide polymorphisms (SNPs) in the 5' flanking region of the hsp70-2 gene in boars. These SNPs have been associated with the quality of semen production during the summer and can, also, be used as markers for semen quality selection in these animals.…”

Section: Discussionmentioning

confidence: 99%

Polymorphism analysis of the hsp70 stress gene in Broiler chickens (Gallus gallus) of different breeds

Mazzi

Ferro

et al. 2003

Genet. Mol. Biol.

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The promoter region and the beginning of the coding region of the hsp70 stress gene were analysed in broiler chickens of a commercial breed (Hubbard-Pettersen), a breed selected for weight gain (PP1) and a non-selected breed (naked-neck Label Rouge). The naked neck gene (Naked neck, Na), which reduces feathering in birds and is thus related to heat resistance, was present in both PP1 and Label Rouge breeds. Genomic DNA was restricted with PstI and Southern blotting analysis of the samples revealed the absence of polymorphic sites for that enzyme in the promoter region and beginning of the coding region of the hsp70 gene of studied birds. PCR-SSCP analysis of these regions, however, indicated the presence of polymorphisms in the beginning of the coding region and the sequencing of the PCR products confirmed and identified two polymorphic sites in this region: a transition A → G in position +258 and a transversion C → G in position +276. Both mutations were considered to be silent, since they did not modify the aminoacid sequence of the protein Hsp70. The promoter region of the hsp70 gene was identical in all studied birds, indicating that the regulation pattern of this gene must be the same in all birds at the promoter region. Three different alleles (hsp70-1, hsp70-2 and hsp70-3) were identified for the hsp70 gene from the observed mutations. The allele hsp70-3 was detected in only two breeds, Hubbard-Pettersen and PP1, but at a low frequency (0,016 and 0,006, respectively).

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Effects of single nucleotide polymorphisms in the 5′-flanking region of heat shock protein 70.2 gene on semen quality in boars

Cited by 51 publications

References 28 publications

Cellular thermotolerance is associated with heat shock protein 70.1 genetic polymorphisms in Holstein lactating cows

Cellular thermotolerance is associated with heat shock protein 70.1 genetic polymorphisms in Holstein lactating cows

High Resolution Light Microscopic Evaluation of Boar Semen Quality Sperm Cytoplasmic Droplet Retention in Relationship with Boar Fertility Parameters

Polymorphism analysis of the hsp70 stress gene in Broiler chickens (Gallus gallus) of different breeds

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